Cheongyeolsaseuptang inhibits production of TNF-α, IL-6 and IL-8 as well as NF-κB activation in human mast cells

Kim, Sujin; Lee, Eon-Jeong; Song, Yoon Kang; Jeong, Hyun‐Ja; Lee, Kang‐Min; Kim, Hyung‐Ryong; Chae, Han-Jung; Shin, Tae‐Yong; Kim, Yun-Kyung; Hong, Seung‐Heon

doi:10.1016/j.jep.2004.10.018

Cited by 9 publications

(6 citation statements)

References 30 publications

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“…Mast cells are major immune cells involved in the release of histamine, and it induces cytokines, such as IL-6, IL-8, and TNF-α in mast cells. [ 17 18 19 20 ] In the present study, peimine reduced cytokines including IL-6, IL-8, and TNF-α expressions in PMACI-stimulated HMC-1 significantly. These cytokines play an important role in triggering the mast cell-mediated allergic inflammatory response.…”

Section: Discussionsupporting

confidence: 52%

Peimine inhibits the production of proinflammatory cytokines through regulation of the phosphorylation of NF-κB and MAPKs in HMC-1 Cells

et al. 2017

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Background:Peimine is a major biologically active component of Fritillaria ussuriensis. Peimine was investigated in chronic inflammation response, but it has not been studied in mast cell-related immediate allergic reaction. The present study aimed to evaluate anti-allergic effect of peimine in human mast cell (HMC-1).Materials and Methods:The effect of peimine on cell viability was measured by MTS assay in HMC-1. Histamine release was investigated in rat peritoneal mast cells (RPMCs). Interleukin (IL)-6, IL-8, and tumor necrosis factor-α (TNF-α) expressions were measured by ELISA assay and reverse transcription-polymerase chain reaction. Mitogen-activated protein kinases (MAPKs) and nuclear factor-kappaB (NF-κB) were examined by Western blot. Passive cutaneous anaphylaxis (PCA) reactions were evaluated using Sprague-Dawley (SD) rats.Results:Peimine inhibited the production of pro-inflammatory cytokines, such as IL-6, IL-8, and TNF-α. Moreover, peimine reduced MAPKs phosphorylation and the nuclear NF-κB expression in PMACI-induced HMC-1. Peimine decreased PCA reactions in rats as well.Conclusion:Our study proved that peimine might be suitable for the treatment of mast cell-derived allergic inflammatory reactions.SUMMARY Peimine inhibited the production of pro-inflammatory cytokines, such as IL-6, IL-8, and TNF-αPeimine reduced MAPKs phosphorylation and the nuclear NF-κB expression in PMACI-induced HMC-1Peimine decreased PCA reactions in ratsPeimine has anti-allergic effect through regulation of pro-inflammatory mechanism on mast cell. Abbreviations used: HMC-1: Human mast cell, MTS: 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, RPMCs: Rat peritoneal mast cells. IL-6: Interleukin 6, IL-8: Interleukin 8, TNF-α: Tumor necrosis factor-α, MAPKs: Mitogen-activated protein kinases; NF-κB: Nuclear factor-kappaB, PCA: Passive cutaneous anaphylaxis reactions, SD: Sprague-Dawley.

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Section: Discussionsupporting

confidence: 52%

Peimine inhibits the production of proinflammatory cytokines through regulation of the phosphorylation of NF-κB and MAPKs in HMC-1 Cells

et al. 2017

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“…In addition, the inhibition of TNF-α, IL-6, IL-8 and NF-κB activation has been shown as an indicator of anti-inflammatory events in mast cells (21,24). Although several traditional herbal medicines have been shown to have inhibitory effects on allergy or inflammation using mast cells (25,26), there is little information with regard to the effects of HCT on mast cell-derived allergic inflammation or the molecular mechanisms involved.…”

Section: Discussionmentioning

confidence: 99%

Houttuynia cordata Thunb inhibits the production of pro-inflammatory cytokines through inhibition of the NFκB signaling pathway in HMC-1 human mast cells

Lee

Seo

Gyungjun

et al. 2013

Molecular Medicine Reports

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Houttuynia cordata Thunb (HCT) is widely used in oriental medicine as a remedy for inflammation. However, at present there is no explanation for the mechanism by which HCT affects the production of inflammatory cytokines. The current study aimed to determine the effect of an essence extracted from HCT on mast cell-mediated inflammatory responses. Inflammatory cytokine production induced by phorbol myristate acetate (PMA) plus a calcium ionophore, A23187, was measured in the human mast cell line, HMC-1, incubated with various concentrations of HCT. TNF-α, IL-6 and IL-8 secreted protein levels were measured using an ELISA assay. TNF-α, IL-6 and IL-8 mRNA levels were measured using RT-PCR analysis. Nuclear and cytoplasmic proteins were examined by western blot analysis. The NF-κB promoter activity was examined by luciferase assay. It was observed that HCT inhibited PMA plus A23187-induced TNF-α and IL-6 secretion and reduced the mRNA levels of TNF-α, IL-6 and IL-8. It was also noted that HCT suppressed the induction of NF-κB activity, inhibited nuclear translocation of NF-κB and blocked the phosphorylation of IκBα in stimulated HMC-1 cells. It was concluded that HCT is an inhibitor of NF-κB and cytokines blocking mast cell-mediated inflammatory responses. These results indicate that HCT may be used for the treatment of mast cell-derived allergic inflammatory diseases.

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“…For example, activation of NF-B by TNF-␣ is required for transcription of the gene encoding endothelial cell adhesion molecules (Collins et al, 1995). Cheongyeolsaseuptang, a traditional Korean medicine, is reported to inhibit the production of TNF-␣, IL-6, and IL-8 and to suppress NF-B activation in mast cells (Kim et al, 2005). CML-1, a Korean folk medicine was found to reduce the adhesive capacities of E-selectin, ICAM-1 and VCAM-1 by inhibiting the IB/NF-B signaling pathway at the level of IB kinase (Mo et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Xia-Bai-San inhibits lipopolysaccharide-induced activation of intercellular adhesion molecule-1 and nuclear factor-kappa B in human lung cells

Lee

Yeh

Kao

et al. 2009

Journal of Ethnopharmacology

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Cheongyeolsaseuptang inhibits production of TNF-α, IL-6 and IL-8 as well as NF-κB activation in human mast cells

Cited by 9 publications

References 30 publications

Peimine inhibits the production of proinflammatory cytokines through regulation of the phosphorylation of NF-κB and MAPKs in HMC-1 Cells

Peimine inhibits the production of proinflammatory cytokines through regulation of the phosphorylation of NF-κB and MAPKs in HMC-1 Cells

Houttuynia cordata Thunb inhibits the production of pro-inflammatory cytokines through inhibition of the NFκB signaling pathway in HMC-1 human mast cells

Xia-Bai-San inhibits lipopolysaccharide-induced activation of intercellular adhesion molecule-1 and nuclear factor-kappa B in human lung cells

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