Chenopodium ficifolium flowers under long days without upregulation of FLOWERING LOCUS T (FT) homologs

Štorchová, Helena; Hubáčková, Helena; Abeyawardana, Oushadee A. J.; Walterová, Jana; Vondráková, Zuzana; Eliášová, Kateřina; Mandák, Bohumil

doi:10.1007/s00425-019-03285-1

Cited by 15 publications

(26 citation statements)

References 47 publications

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“…Interestingly, ACT has been validated as a suitable reference for diurnal expression studies in Chenopodium ficifolium [39]. In our study, ACT showed to be the best reference according to NormFinder, but this result was not supported by geNorm nor NormFinder methods.…”

Section: Plos Onecontrasting

confidence: 61%

Validation of suitable genes for normalization of diurnal gene expression studies in Chenopodium quinoa

et al. 2021

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Quinoa depicts high nutritional quality and abiotic stress resistance, attracting strong interest in the last years. To unravel the function of candidate genes for agronomically relevant traits, studying their transcriptional activities by RT-qPCR is an important experimental approach. The accuracy of such experiments strongly depends on precise data normalization. To date, validation of potential candidate genes for normalization of diurnal expression studies has not been performed in C. quinoa. We selected eight candidate genes based on transcriptome data and literature survey, including conventionally used reference genes. We used three statistical algorithms (BestKeeper, geNorm and NormFinder) to test their stability and added further validation by a simulation-based strategy. We demonstrated that using different reference genes, including those top ranked by stability, causes significant differences among the resulting diurnal expression patterns. Our results show that isocitrate dehydrogenase enzyme (IDH-A) and polypyrimidine tract-binding protein (PTB) are suitable genes to normalize diurnal expression data of two different quinoa accessions. Moreover, we validated our reference genes by normalizing two known diurnally regulated genes, BTC1 and BBX19. The validated reference genes obtained in this study will improve the accuracy of RT-qPCR data normalization and facilitate gene expression studies in quinoa.

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Section: Plos Onecontrasting

confidence: 61%

Validation of suitable genes for normalization of diurnal gene expression studies in Chenopodium quinoa

et al. 2021

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“…Quinoa, as a polyploid species with a short evolutionary history, carries multiple copies of FT homologs. Therefore, we focused on genes that are highly related to proven FT orthologues from the Amaranthaceae family (Drabešov a et al, 2014;Pin et al, 2010;Štorchov a et al, 2019). We selected five FT homologs that had already been found in the reference genome sequence (Jarvis et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, in C . ficifolium under LDs, the expression of FT homologs was negligible, although plants flowered regularly (Štorchová et al, 2019). It is tempting to speculate that other regulatory pathways control flowering in quinoa under LDs.…”

Section: Discussionmentioning

confidence: 99%

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Haplotype variations of major flowering time genes in quinoa unveil their role in the adaptation to different environmental conditions

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Maldonado-Taipe

et al. 2021

Plant Cell & Environment

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Response to photoperiod is of major importance in crop production. It defines the adaptation of plants to local environments. Quinoa is a short-day plant which had been domesticated in the Andeans regions. We wanted to understand the adaptation to long-day conditions by studying orthologues of two major flowering time regulators of Arabidopsis, FLOWERING LOCUS T (FT) and CONSTANS (CO) in quinoa accessions with contrasting photoperiod response. By searching the quinoa reference genome sequence, we identified 24 FT and six CO homologs. CqFT genes displayed remarkably different expression patterns between long-and short-day conditions, whereas the influence of the photoperiod on CqCOL expressions was moderate. Cultivation of 276 quinoa accessions under short-and long-day conditions revealed great differences in photoperiod sensitivity. After sequencing their genomes, we identified large sequence variations in 12 flowering time genes. We found nonrandom distribution of haplotypes across accessions from different geographical origins, highlighting the role of CqFT and CqCOL genes in the adaptation to different day-length conditions. We identified five haplotypes causing early flowering under long days. This study provides assets for quinoa breeding because superior haplotypes can be assembled in a predictive breeding approach to produce well-adapted early flowering lines under long-day photoperiods.

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“…The accession C. ficifolium 459 was originally collected in Central Asia [2] . The plants were cultivated in the Institute of Experimental Botany greenhouse and propagated by self-pollination.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

The transcriptomic (RNA-Sequencing) datasets collected in the course of floral induction in Chenopodium ficifolium 459

et al. 2022

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Chenopodium ficifolium flowers under long days without upregulation of FLOWERING LOCUS T (FT) homologs

Cited by 15 publications

References 47 publications

Validation of suitable genes for normalization of diurnal gene expression studies in Chenopodium quinoa

Validation of suitable genes for normalization of diurnal gene expression studies in Chenopodium quinoa

Haplotype variations of major flowering time genes in quinoa unveil their role in the adaptation to different environmental conditions

The transcriptomic (RNA-Sequencing) datasets collected in the course of floral induction in Chenopodium ficifolium 459

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