Chemotherapeutic Effects on Mammalian Tumor Cells. I. Modification of Leukemia L1210 Growth Kinetics and Karyotype With an Alkylating Agent

Johnson, Ralph E.; Zelen, Marvin; Kemp, N. H.

doi:10.1093/jnci/34.2.277

Cited by 25 publications

(5 citation statements)

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“…It seems likely therefore that treatment of a cell population with an alkylating agent may induce a broader range of sensitivities to the drug than was present in the original population. Similar results were obtained in cell culture (Fox and Nias, 1968) and from L1210 in vivo (Johnson et al, 1965) with other alkylating agents.…”

Section: Sulphydryl Group Assayssupporting

confidence: 83%

The sensitivity of a yoshida sarcoma to methylene dimethane sulphonate

Fox

1969

Intl Journal of Cancer

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Section: Sulphydryl Group Assayssupporting

confidence: 83%

The sensitivity of a yoshida sarcoma to methylene dimethane sulphonate

Fox

1969

Intl Journal of Cancer

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“…Further, chemotherapy may result in prolongation of the TG, either as a result of selective killing of more rapidly dividing cells or of slowing the growth of surviving blasts that are (sublethally) damaged (55). Such an effect has previously been demonstrated in L1210 mouse leukemia (70) and in the free ascitic cancer cells in a patient with ovarian carcinoma (15).…”

Section: Generation Timementioning

confidence: 99%

“…Killmann and co-workers (9) estimated that the total number of dividing blasts in one patient with acute leukemia was 2.5 x 1011; the patient's weight was not given, but if it is assumed to have been 70 kg, this would be about 3.5 X 109 blasts per kg. In our patients the values were 1.6 and 2.6 x 109 per kg.…”

Section: Total Number Of Dividing Blastsmentioning

confidence: 99%

Studies of Cellular Proliferation in Human Leukemia. I. Estimation of Growth Rates of Leukemic and Normal Hematopoietic Cells in Two Adults with Acute Leukemia Given Single Injections of Tritiated Thymidine *

Clarkson¹,

Ohkita²,

K³

et al. 1967

J. Clin. Invest.

162

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Summary. Two adults with rapidly progressive acute myeloblastic and myelomonoblastic leukemia were given single injections of tritiated thymidine, and measurements were made of the growth rates of their leukemic and normal hematopoietic cells by radioautographic methods. Although almost all leukemic blasts in both marrow and blood were metabolically active as shown by their ability to incorporate tritiated uridine and leucine in Vitro, only 5.6% and 6.1% ol the blasts in the marrow and even fewer in the blood incorporated tritiated thymidine. The mitotic indexes of the marrow blasts were 0.66% and 0.52%; no circulating blasts were dividing. The mean generation times of the actively proliferating blasts were estimated to be 49 and 83 hours. This cannot be equated with the doubling time of the total leukemic population as there is evidence that many blasts fail to continue dividing and die. The mean durations of the phases of the blasts' mitotic cycles were as follows: DNA synthesis (S) = 22 and 19 hours, premitosis (G2) = 3 hours, mitosis (M) = 0.47 and 0.62 hour (minimal estimates), and postmitosis (G1) = 24 and 61 hours. In both patients the maximal mean transit time of the blasts in the blood was 36 hours, and the minimal numbers of actively dividing blasts present were 1.6 and 2.6 X 109 per kg of body weight.Estimates were also made of the rates of proliferation and maturation of the residual normal erythrocytic and granulocytic cells in these two patients. Although total production was markedly diminished because of reduction in the number of normal elements, the relatively few remaining normal cells appeared to be dividing and maturing at rates that are about the same or only slightly slower than those found in normal subjects.We conclude that the main reason leukemic blasts displace normal hematopoietic precursors in acute leukemia is that the blasts largely fail to differentiate. Many die but many others persist in the marrow and elsewhere as primitive cells and continue to proliferate. As the blasts accumulate, they

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“…M. Kollmorgen potential chemotherapeutic value in man (Leiter and Schneiderman, 1959;Skipper et al, 1967;Skipper, 1967;Johnson et al, 1965;Leiter et al, 1964). M. Kollmorgen potential chemotherapeutic value in man (Leiter and Schneiderman, 1959;Skipper et al, 1967;Skipper, 1967;Johnson et al, 1965;Leiter et al, 1964).…”

Section: Bacillus C Allmette-gu~rinmentioning

confidence: 99%

Histologic evaluation of L1210 leukemia in treated and untreated mice

Glass

Glassell

Killion

et al. 1977

Cancer Immunol Immunother

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BDFI mice bearing L1210 leukemia were treated with chemotherapy or in combination with neuraminidase-treated cells and BCG. Histological evaluation was done on these mice at various intervals after therapy in order to determine the rate and extent of metastatic involvement in various tissues and organs. Results were compared to tumor-bearing mice which were not treated. In all animals, tissues were classed as having minimal involvement, moderate involvement or maximal involvement based on a scale of 0 through 4. Results indicated that: (a) mice which were long term survivors did not completely reject t~eir tumor for weeks after treatment with chemotherapy and immunotherapy; (b) complete tumor rejection did not indicate a restoration of normal tissue integrity; and (e) failure of ehemotherapy-immunotherapy had no consistent pathology, but was probably due to tumor distribution rather then tumor burden per se.

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Chemotherapeutic Effects on Mammalian Tumor Cells. I. Modification of Leukemia L1210 Growth Kinetics and Karyotype With an Alkylating Agent

Cited by 25 publications

References 0 publications

The sensitivity of a yoshida sarcoma to methylene dimethane sulphonate

The sensitivity of a yoshida sarcoma to methylene dimethane sulphonate

Studies of Cellular Proliferation in Human Leukemia. I. Estimation of Growth Rates of Leukemic and Normal Hematopoietic Cells in Two Adults with Acute Leukemia Given Single Injections of Tritiated Thymidine *

Histologic evaluation of L1210 leukemia in treated and untreated mice

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