Chemotaxonomy of Triterpenoid Pattern of HPLC of Ganoderma lucidum and Ganoderma tsugae

Abstract

Three strains of Ganoderma tsugae (CCRC36065, CCRC37034, CCRC37038) and three strains of Ganoderma lucidum (CCRC36021, CCRC37029, CCRC37033) were cultivated. Their triterpenoid patterns of the fruit body were analyzed by reverse phase HPLC using a gradient elution of acetonitrile/2% acetic acid (1/4 and 1/2). The triterpenoid patterns of G. tsugae and G. lucidum are different. But similar 2 and 3 dimensional patterns are obtained among three strains of G. tsugae. Different patterns are found among different strains of G. lucidum. Ganoderic acid A(1), B(2), C(3) and D(4) were isolated from the ethanol extract of G. tsugae.

The anti‐invasive effect of lucidenic acids isolated from a new Ganoderma lucidum strain

“…The G. lucidum strain (YK-02), which was preserved in Mycelium Center of Biotechnology R & D Institute (Shnang Hor Group, Tainan Hsien, Taiwan), was grown to fruiting body according to the report of Chen et al [10]. It has been identified by Dr. Shen-Hwa Wu (National Museum of Natural Science, Taiwan), using morphology characterization and DNA profiles.…”

“…Crude triterpenoids of G. lucidum (YK-02) were separated according to the method of Chen et al [10]. Briefly, G. lucidum (YK-02) powder was extracted with 50% ethanol for 1 h. The mixture was filtered, and the filtrate was evaporated to dryness with a rotary evaporator.…”

From 2000years of Ganoderma lucidum to recent developments in nutraceuticals

“…In a study reported in 1999, Chen et al (1999) were able to use an analysis of triterpenoids to differentiate between G. tsugae and G. lucidum. However, although the three G. tsugae strains tested presented with similar spectral profiles, the three G. lucidum strains tested had profiles that were both very different from those generated from G. tsugae strains, and from each other.…”

The hepatoprotective activity against ethanol-induced cytotoxicity by aqueous extract of Antrodia cinnamomea

“…Triterpenoid content was analyzed (Chang et al, 2006;Chen et al, 1999); 10 g of freeze-dried mycelia was immersed in 150 mL ethanol and incubated in a 65 8C water bath for 1 h. The hot solvent extract was filtered with Whatman No. 5 filter paper to collect the solvent.…”