Chemotaxonomy of Quaternary benthic foraminifera using amino acids

Haugen, John‐Erik; Sejrup, Hans Petter; Vogt, N.B.

doi:10.2113/gsjfr.19.1.38

Cited by 18 publications

(13 citation statements)

References 2 publications

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“…During the demineralization with cold 6.5 M acetic acid, the calcite test powder dissolved and exposed the organic layer(s) that were previously protected by the mineral (King & Hare, 1972a;Weiner & Erez, 1984;Haugen et al, 1989;Robbins & Healy-Williams, 1991;Stathoplos & Tuross, 1994). During the demineralization with cold 6.5 M acetic acid, the calcite test powder dissolved and exposed the organic layer(s) that were previously protected by the mineral (King & Hare, 1972a;Weiner & Erez, 1984;Haugen et al, 1989;Robbins & Healy-Williams, 1991;Stathoplos & Tuross, 1994).…”

Section: Manmentioning

confidence: 99%

“…Our current knowledge of the macromolecular components (e.g., proteins, sugars, and lipids) in the organic matrix of foraminiferal shells is very poor (Hedley, 1963;Banner et al, 1973;Weiner & Erez, 1984). Previous research has focused mainly on protein components in terms of amino acid composition (King & Hare, 1972a;Weiner & Erez, 1984;Haugen et al, 1989;Robbins & Donachy, 1991;Robbins & Healy-Williams, 1991;Stathoplos & Tuross, 1994). Very few studies have tried to identify the amino acid sequences of matrix shell proteins in foraminifera (Robbins et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, very few taxa and species have been examined. For example, most studies investigated fossil planktonic foraminifera (King & Hare, 1972a;Haugen et al, 1989;Robbins & Brew, 1990;Robbins & Donachy, 1991;Robbins & Healy-Williams, 1991;Stathoplos & Tuross, 1994). Prior to this study, the only living tropical benthic foraminifer to be analyzed in this way was Heterostegina depressa d'Orbigny, 1826 (Weiner & Erez, 1984).…”

Section: Introductionmentioning

confidence: 99%

“…(B) Detail of A) describing the building of a new chamber. Previous research has focused mainly on protein components in terms of amino acid composition (King & Hare, 1972a;Weiner & Erez, 1984;Haugen et al, 1989;Robbins & Donachy, 1991;Robbins & Healy-Williams, 1991;Stathoplos & Tuross, 1994). In this space, an organic matrix organized in a thin layer, called the primary organic membrane (POM), may act as template or mold for the nucleation and the precipitation of CaCO 3 on both its sides, resulting in inner and outer layers of calcite.…”

mentioning

confidence: 99%

“…In this space, an organic matrix organized in a thin layer, called the primary organic membrane (POM), may act as template or mold for the nucleation and the precipitation of CaCO 3 on both its sides, resulting in inner and outer layers of calcite. For example, most studies investigated fossil planktonic foraminifera (King & Hare, 1972a;Haugen et al, 1989;Robbins & Brew, 1990;Robbins & Donachy, 1991;Robbins & Healy-Williams, 1991;Stathoplos & Tuross, 1994). These modified seawater vacuoles are exocytosed into the delimited biomineralization space where CaCO 3 is precipitated over the existing shell.…”

mentioning

confidence: 99%

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Biomineralization ofSchlumbergerella floresiana, a significant carbonate‐producing benthic foraminifer

et al. 2014

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Most foraminifera that produce a shell are efficient biomineralizers. We analyzed the calcitic shell of the large tropical benthic foraminifer Schlumbergerella floresiana. We found a suite of macromolecules containing many charged and polar amino acids and glycine that are also abundant in biomineralization proteins of other phyla. As neither genomic nor transcriptomic data are available for foraminiferal biomineralization yet, de novo-generated sequences, obtained from organic matrices submitted to ms blast database search, led to the characterization of 156 peptides. Very few homologous proteins were matched in the proteomic database, implying that the peptides are derived from unknown proteins present in the foraminiferal organic matrices. The amino acid distribution of these peptides was queried against the uniprot database and the mollusk uniprot database for comparison. The mollusks compose a well-studied phylum that yield a large variety of biomineralization proteins. These results showed that proteins extracted from S. floresiana shells contained sequences enriched with glycine, alanine, and proline, making a set of residues that provided a signature unique to foraminifera. Three of the de novo peptides exhibited sequence similarities to peptides found in proteins such as pre-collagen-P and a group of P-type ATPases including a calcium-transporting ATPase. Surprisingly, the peptide that was most similar to the collagen-like protein was a glycine-rich peptide reported from the test and spine proteome of sea urchin. The molecules, identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry analyses, included acid-soluble N-glycoproteins with its sugar moieties represented by high-mannose-type glycans and carbohydrates. Describing the nature of the proteins, and associated molecules in the skeletal structure of living foraminifera, can elucidate the biomineralization mechanisms of these major carbonate producers in marine ecosystems. As fossil foraminifera provide important paleoenvironmental and paleoclimatic information, a better understanding of biomineralization in these organisms will have far-reaching impacts.

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