Chemotaxonomy of bacteria by comprehensive GC and GC‐MS in electron impact and chemical ionisation mode

David, Frank; Tienpont, Bart; Sandra, Pat

doi:10.1002/jssc.200800215

Cited by 48 publications

(41 citation statements)

References 16 publications

(12 reference statements)

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“…S. malto philia has a complex fatty acid profile and different OH-acids detected in the sample. Similiar results were reported by David et al (2008). The predominance of terminally methylbranched iso and anteiso fatty acids having 12 to 17 carbons is a characteristic observed in all Bacillus species.…”

supporting

confidence: 72%

Isolation and Characterization of α-Endosulfan Degrading Bacteria from the Microflora of Cockroaches

Özdal

Ozdal

Alguri

2016

Polish Journal of Microbiology

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A b s t r a c tExtensive applications of organochlorine pesticides like endosulfan have led to the contamination of soil and environments. Five different bacteria were isolated from cockroaches living in pesticide contaminated environments. According to morphological, physiological, biochemical properties, and total cellular fatty acid profile by Fatty Acid Methyl Esters (FAMEs), the isolates were identified as Pseudomonas aeruginosa G1, Stenotrophomonas maltophilia G2, Bacillus atrophaeus G3, Citrobacter amolonaticus G4 and Acinetobacter lwoffii G5. This is the first study on the bacterial flora of Blatta orientalis evaluated for the biodegradation of α-endosulfan. After 10 days of incubation, the biodegradation yields obtained from P. aeruginosa G1, S. maltophilia G2, B. atrophaeus G3, C. amolonaticus G4 and A. lwoffii G5 were 88.5% , 85.5%, 64.4%, 56.7% and 80.2%, respectively. As a result, these bacterial strains may be utilized for biodegradation of endosulfan polluted soil and environments. 64developed resistance to pesticides. If this is true, we can isolate new and effective α-endosulfan degrading bacteria from cockroaches' microflora and these isolates can be used for the biological treatment of waters and soils polluted with endosulfan and other insecticides. Experimental Material and MethodsChemicals. α-endosulfan and reagents were purchased from Sigma-Aldrich (St Louis, MO, USA). All solvents used were of the highest analytical grade and were employed without further purification. The stock α-endosulfan solution was prepared in acetone and used for all the experiments. The chemical structure and the most important physical parameters of endosulfan are summarized in Fig. 1 O, 24 (Siddique et al., 2003). After 7 days, 5 ml of each culture was re-inoculated into new α-endosulfan-NSM medium and further incubated at 30°C for 7 days. This subculture was repeated under the same culture conditions, and then an aliquot (0.2 ml) from each culture was applied to solid α-endosulfan-NSM for isolation of single colonies.Identification of endosulfan degrading microorganisms. α-endosulfan degrading bacterial isolates were identified by various tests, such as pigment formation, Gram staining, nitrate reduction, catalase and oxidase tests, and starch hydrolysis. Biochemical reactions were conducted according to Benson (2001). Biochemical activities were determined according to the recommended scheme of Bergey's Manual of Determinative Bacteriology (Holt et al., 1994). Identification was confirmed by the fatty acid analysis for all bacterial isolates. Fatty acid methyl ester (FAME) profiles of each bacterial strain were identified by comparing the commercial databases (Tripticase Soy Broth Agar 40) with the MIS software package. The identity of bacterial strains was revealed by computer comparison of FAME profiles of the unknown test strains with those in the library. FAMEs were separated by gas chromatography (HP6890, Hewlett Packard, Palo Alto, CA, USA) with a fused-silica capillary column (25 m × 0.2 mm) with cr...

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supporting

confidence: 72%

Isolation and Characterization of α-Endosulfan Degrading Bacteria from the Microflora of Cockroaches

Özdal

Ozdal

Alguri

2016

Polish Journal of Microbiology

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“…Enrichment of the analytes by the PDMS layer is determined by the PDMS-water partition coefficient (K PDMS/w ) or the octanol -water distribution coefficient (K o/w ) of the analytes. High recoveries (i. e., nearly 100%) can be achieved from aqueous solutions when the log K o/w value exceeds 2.7 [17]. Therefore, androgenic hormones are highly suitable for extraction by SBSE or SPME (i. e., log K o/w values A 3; Kowwin [18]).…”

Section: Introductionmentioning

confidence: 99%

Quantification of testosterone and epitestosterone in human urine samples by stir bar sorptive extraction – thermal desorption – gas chromatography/mass spectrometry: Application to HIV‐positive urine samples

Stopforth

Grobbelaar²,

Crouch

et al. 2007

J of Separation Science

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A simple method is described for the measurement of testosterone (T) and epitestosterone (ET) in human urine samples. The deconjugated steroids were extracted directly from the samples by stir bar sorptive extraction (SBSE) and derivatized in situ on the stir bar by headspace acylation prior to thermal desorption and GC/MS. Extraction and derivatization parameters, namely salt addition, temperature, and time, were optimized to improve the recovery of T and ET by SBSE. The limits of quantification (S/N 10) were 0.9 ng/mL for T and 2.8 ng/mL for ET. Quantification of the steroids in urine samples was performed using standard addition to avoid the influence of matrix effects. The method was applied for the measurement of urinary T and ET in a group of healthy volunteers and HIV+ patients. Decreased levels of T were detected in the HIV+ group, whereas the excretion of ET was comparable for the two groups. Further clinical research is required to elucidate the biomarker significance of the T/ET ratio in HIV infection.

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“…In this respect, comprehensive two dimensional gas chromatography (GC × been applied for the analysis of FAMEs in microorganism [16][17][18], fish and vegetable oil [19][20][21][22][23][24][25][26][27][28], human plasma [29] and milk fat [30]. Comprehensive two dimensional gas chromatography is especially useful for group type separation, combining the carbon number separation and the separation according to the number of double bonds.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of ionic liquid stationary phases for one dimensional gas chromatography–mass spectrometry and comprehensive two dimensional gas chromatographic analyses of fatty acids in marine biota

David

Lynen

et al. 2011

Journal of Chromatography A

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Chemotaxonomy of bacteria by comprehensive GC and GC‐MS in electron impact and chemical ionisation mode

Cited by 48 publications

References 16 publications

Isolation and Characterization of α-Endosulfan Degrading Bacteria from the Microflora of Cockroaches

Isolation and Characterization of α-Endosulfan Degrading Bacteria from the Microflora of Cockroaches

Quantification of testosterone and epitestosterone in human urine samples by stir bar sorptive extraction – thermal desorption – gas chromatography/mass spectrometry: Application to HIV‐positive urine samples

Evaluation of ionic liquid stationary phases for one dimensional gas chromatography–mass spectrometry and comprehensive two dimensional gas chromatographic analyses of fatty acids in marine biota

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