Chemotaxis of Burkholderia sp. Strain SJ98 towards chloronitroaromatic compounds that it can metabolise

Abstract

BackgroundBurkholderia sp. strain SJ98 is known for its chemotaxis towards nitroaromatic compounds (NACs) that are either utilized as sole sources of carbon and energy or co-metabolized in the presence of alternative carbon sources. Here we test for the chemotaxis of this strain towards six chloro-nitroaromatic compounds (CNACs), namely 2-chloro-4-nitrophenol (2C4NP), 2-chloro-3-nitrophenol (2C3NP), 4-chloro-2-nitrophenol (4C2NP), 2-chloro-4-nitrobenzoate (2C4NB), 4-chloro-2-nitrobenzoate (4C2NB) and 5-chloro-2-nitrobenzoate (5C2NB), and examine its relationship to the degradation of such compounds.ResultsStrain SJ98 could mineralize 2C4NP, 4C2NB and 5C2NB, and co-metabolically transform 2C3NP and 2C4NB in the presence of an alternative carbon source, but was unable to transform 4C2NP under these conditions. Positive chemotaxis was only observed towards the five metabolically transformed CNACs. Moreover, the chemotaxis was induced by growth in the presence of the metabolisable CNAC. It was also competitively inhibited by the presence of nitroaromatic compounds (NACs) that it could metabolise but not by succinate or aspartate.ConclusionsBurkholderia sp. strain SJ98 exhibits metabolic transformation of, and inducible chemotaxis towards CNACs. Its chemotactic responses towards these compounds are related to its previously demonstrated chemotaxis towards NACs that it can metabolise, but it is independently inducible from its chemotaxis towards succinate or aspartate.

Metabolism Dependent Chemotaxis of Pseudomonas aeruginosa N1 Towards Anionic Detergent Sodium Dodecyl Sulfate

“…Chemotaxis of Burkholderia sp. strain SJ98 towards different NACs has also been reported [22]. It was observed that strain SJ98 was chemotactic towards various substituted nitrophenols [24].…”

“…10 and 30 mg SDS was placed in the center of two petri plates and chemotactic response was observed after 9 h of incubation at 37°C. In control plates, glucose (10 mg) was placed instead of SDS [22]. To evaluate the response of SDS grown cells towards both SDS and Glucose, SDS grown cells were mixed with semi solid PBM medium and poured in petri plates according to the drop plate assay method.…”

“…Formation of rings was observed after 9 h of incubation. In control plates, glucose (1 g/L) was added instead of SDS [22].…”

Identification of CtpL as a Chromosomally Encoded Chemoreceptor for 4-Chloroaniline and Catechol in Pseudomonas aeruginosa PAO1

“…Bacterial chemotactic attraction to environmental pollutants can enhance biodegradation rates by increasing pollutant bioavailability, and this potentially leads to an improvement in bioremediation efficiency (1). Several studies have reported the isolation and characterization of bacteria with a chemotactic response toward a variety of hazardous aromatic pollutants, including pesticides (2, 3), petroleum-associated monoaromatic (4)(5)(6), and polyaromatic (7,8) and aromatic (9,10) derivatives. However, such information is relatively scarce for some of the recently recognized recalcitrant xenobiotics, such as chloronitroaromatics, furan, and chlorinated anilines (9,11).…”

“…Several studies have reported the isolation and characterization of bacteria with a chemotactic response toward a variety of hazardous aromatic pollutants, including pesticides (2, 3), petroleum-associated monoaromatic (4)(5)(6), and polyaromatic (7,8) and aromatic (9,10) derivatives. However, such information is relatively scarce for some of the recently recognized recalcitrant xenobiotics, such as chloronitroaromatics, furan, and chlorinated anilines (9,11). Despite the numerous reports of the isolation and phenotypic characterization of chemotactic bacteria, the specificity of the bacterial chemotactic response to chemicals is actually determined by their chemoreceptors (12).…”