Chemotaxis of a model organism: progress with Dictyostelium

Nichols, John Me; Veltman, Douwe M.; Kay, Robert R.

doi:10.1016/j.ceb.2015.06.005

Cited by 84 publications

(77 citation statements)

References 51 publications

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“…21 Interestingly, even in media containing a uniform concentration of fMLP, HL-60 cells localize F-actin to a portion of the cell and myosin IIA or RhoA to the opposite side. 22,23 In Dictyostelium cells, localization of phosphatidylinositol 3,4,5-trisphosphate (PIP 3 ) plays a role in the generation of migrating polarity, not only in the presence of extracellular cAMP, 24,25 but also in its absence.…”

Section: Discussionmentioning

confidence: 99%

Fast-crawling cell types migrate to avoid the direction of periodic substratum stretching

Okimura

Ueda

Sakumura

et al. 2016

Cell Adhesion & Migration

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To investigate the relationship between mechanical stimuli from substrata and related cell functions, one of the most useful techniques is the application of mechanical stimuli via periodic stretching of elastic substrata. In response to this stimulus, Dictyostelium discoideum cells migrate in a direction perpendicular to the stretching direction. The origins of directional migration, higher migration velocity in the direction perpendicular to the stretching direction or the higher probability of a switch of migration direction to perpendicular to the stretching direction, however, remain unknown. In this study, we applied periodic stretching stimuli to neutrophil-like differentiated HL-60 cells, which migrate perpendicular to the direction of stretch. Detailed analysis of the trajectories of HL-60 cells and Dictyostelium cells obtained in a previous study revealed that the higher probability of a switch of migration direction to that perpendicular to the direction of stretching was the main cause of such directional migration. This directional migration appears to be a strategy adopted by fast-crawling cells in which they do not migrate faster in the direction they want to go, but migrate to avoid a direction they do not want to go.

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Section: Discussionmentioning

confidence: 99%

Fast-crawling cell types migrate to avoid the direction of periodic substratum stretching

Okimura

Ueda

Sakumura

et al. 2016

Cell Adhesion & Migration

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“…This technique has been used to study the migration of neutrophils and Dictyostelium discoideum cells and allowed an exploration of the key molecular pathways involved in chemical sensing (Kay et al. , 2008; Nichols et al. , 2015).…”

Section: Introductionmentioning

confidence: 99%

Method to study cell migration under uniaxial compression

Srivastava

Kay

Kabla

2017

MBoC

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“…Dictyostelium and Acanthamoeba are two amoebae of general interest because Dictyostelium has been used as a model for eukaryotic cell movement and differentiation and Acanthamoeba has been documented as a human pathogen (Annesley and Fisher 2009; Artemenko et al 2014; Janetopoulos and Firtel 2008; Khan 2003; Lam et al 1997; Nichols et al 2015; Walochnik et al 2008; Williams 2010). Both amoebae inhabit soil environments and feed on bacteria and other microbes (Douglas et al 2013; Khan 2006; Sawyer 1989).…”

Section: Introductionmentioning

confidence: 99%

Acanthamoeba and Dictyostelium Use Different Foraging Strategies

Kuburich

Adhikari

Hadwiger

2016

Protist

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Amoeba often use cell movement as a mechanism to find food, such as bacteria, in their environment. The chemotactic movement of the soil amoeba Dictyostelium to folate or other pterin compounds released by bacteria is a well-documented foraging mechanism. Acanthamoeba can also feed on bacteria but relatively little is known about the mechanism(s) by which this amoeba locates bacteria. Acanthamoeba movement in the presence of folate or bacteria was analyzed in above agar assays and compared to that observed for Dictyostelium. The overall mobility of Acanthamoeba was robust like that of Dictyostelium but Acanthamoeba did not display a chemotactic response to folate. In the presence of bacteria, Acanthamoeba only showed a marginal bias in directed movement whereas Dictyostelium displayed a strong chemotactic response. A comparison of genomes revealed that Acanthamoeba and Dictyostelium share some similarities in G protein signaling components but that specific G proteins used in Dictyostelium chemotactic responses were not present in current Acanthamoeba genome sequence data. The results of this study suggest that Acanthamoeba does not use chemotaxis as the primary mechanism to find bacterial food sources and that the chemotactic responses of Dictyostelium to bacteria may have co-evolved with chemotactic responses that facilitate multicellular development.

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Chemotaxis of a model organism: progress with Dictyostelium

Cited by 84 publications

References 51 publications

Fast-crawling cell types migrate to avoid the direction of periodic substratum stretching

Fast-crawling cell types migrate to avoid the direction of periodic substratum stretching

Method to study cell migration under uniaxial compression

Acanthamoeba and Dictyostelium Use Different Foraging Strategies

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