Chemotaxis: insights from the extending pseudopod

Haastert, Peter J.M. van

doi:10.1242/jcs.071118

Cited by 47 publications

(52 citation statements)

References 53 publications

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“…Directional cues induce pseudopodia oriented toward the gradient, which includes selective retraction, oriented extension, and suppression of de novo pseudopodia formation (29). To move in the direction of a gradient, leukocytes must form many pseudopodia at the side of the cell that is facing the gradient.…”

Section: Discussionmentioning

confidence: 99%

Factor Associated with Neutral Sphingomyelinase Activity Mediates Navigational Capacity of Leukocytes Responding to Wounds and Infection: Live Imaging Studies in Zebrafish Larvae

Boecke

Sieger

Neacsu³

et al. 2012

The Journal of Immunology

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Factor associated with neutral sphingomyelinase activity (FAN) is an adaptor protein that specifically binds to the p55 receptor for TNF (TNF-RI). Our previous investigations demonstrated that FAN plays a role in TNF-induced actin reorganization by connecting the plasma membrane with actin cytoskeleton, suggesting that FAN may impact on cellular motility in response to TNF and in the context of immune inflammatory conditions. In this study, we used the translucent zebrafish larvae for in vivo analysis of leukocyte migration after morpholino knockdown of FAN. FAN-deficient zebrafish leukocytes were impaired in their migration toward tail fin wounds, leading to a reduced number of cells reaching the wound. Furthermore, FAN-deficient leukocytes show an impaired response to bacterial infections, suggesting that FAN is generally required for the directed chemotactic response of immune cells independent of the nature of the stimulus. Cell-tracking analysis up to 3 h after injury revealed that the reduced number of leukocytes is not due to a reduction in random motility or speed of movement. Leukocytes from FAN-deficient embryos protrude pseudopodia in all directions instead of having one clear leading edge. Our results suggest that FAN-deficient leukocytes exhibit an impaired navigational capacity, leading to a disrupted chemotactic response.

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Section: Discussionmentioning

confidence: 99%

Factor Associated with Neutral Sphingomyelinase Activity Mediates Navigational Capacity of Leukocytes Responding to Wounds and Infection: Live Imaging Studies in Zebrafish Larvae

Boecke

Sieger

Neacsu³

et al. 2012

The Journal of Immunology

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“…Pseudopodia are essential for cell movement since they determine the trajectory, the direction, and the speed of the cell. Also, adjacent cells may coordinate pseudopodia extensions to contribute to collective cell migration (Van Haastert 2010) or contact guidance (Weijer 2009).…”

Section: Pseudopodia Characteristicsmentioning

confidence: 99%

Cell Migration with Multiple Pseudopodia: Temporal and Spatial Sensing Models

Allena

2013

Bull Math Biol

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is an open access repository that collects the work of Arts et Métiers ParisTech researchers and makes it freely available over the web where possible. Abstract Cell migration triggered by pseudopodia (or "false feet") is the most used method of locomotion. A 3D finite element model of a cell migrating over a 2D substrate is proposed, with a particular focus on the mechanical aspects of the biological phenomenon. The decomposition of the deformation gradient is used to reproduce the cyclic phases of protrusion and contraction of the cell, which are tightly synchronized with the adhesion forces at the back and at the front of the cell, respectively. First, a steady active deformation is considered to show the ability of the cell to simultaneously initiate multiple pseudopodia. Here, randomness is considered as a key aspect, which controls both the direction and the amplitude of the false feet. Second, the migration process is described through two different strategies: the temporal and the spatial sensing models. In the temporal model, the cell "sniffs" the surroundings by extending several pseudopodia and only the one that receives a positive input will become the new leading edge, while the others retract. In the spatial model instead, the cell senses the external sources at different spots of the membrane and only protrudes one pseudopod in the direction of the most attractive one.

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“…Our model indicates that cancer development may be perceived as a series of events that ultimately leads to an activated cellular state. Considering that the production of PI(3,4,5)P 3 parallels pseudopod extension (Huang et al, 2003;Van Haastert, 2010), a certain loss of PTEN should be tolerable because no apparent pseudopod extension occurs and cells would remain at rest (indicated by the horizontal curves in Fig. 8).…”

Section: Discussionmentioning

confidence: 99%