Chemotaxis in Escherichia coli analysed by Three-dimensional Tracking

Berg, Howard C.; Brown, Douglas A.

doi:10.1038/239500a0

Cited by 2,009 publications

(1,973 citation statements)

References 9 publications

Supporting

106

Mentioning

1,840

Contrasting

Unclassified

Order By: Relevance

“…3D tracking results of bacterial swimming motion indicate that their speed during the running states is nearly constant, but it drops significantly or almost to zero during the tumble states 27. Following from the linear force‐speed relation characterized by Stoke's law, we can conclude that the propulsive force of the running states is approximately constant, while the propulsive force is relatively negligible during the tumble states.…”

Section: Introductionmentioning

confidence: 73%

Propulsion and Chemotaxis in Bacteria‐Driven Microswimmers

2017

View full text Add to dashboard Cite

Despite the large body of experimental work recently on biohybrid microsystems, few studies have focused on theoretical modeling of such systems, which is essential to understand their underlying functioning mechanisms and hence design them optimally for a given application task. Therefore, this study focuses on developing a mathematical model to describe the 3D motion and chemotaxis of a type of widely studied biohybrid microswimmer, where spherical microbeads are driven by multiple attached bacteria. The model is developed based on the biophysical observations of the experimental system and is validated by comparing the model simulation with experimental 3D swimming trajectories and other motility characteristics, including mean squared displacement, speed, diffusivity, and turn angle. The chemotaxis modeling results of the microswimmers also agree well with the experiments, where a collective chemotactic behavior among multiple bacteria is observed. The simulation result implies that such collective chemotaxis behavior is due to a synchronized signaling pathway across the bacteria attached to the same microswimmer. Furthermore, the dependencies of the motility and chemotaxis of the microswimmers on certain system parameters, such as the chemoattractant concentration gradient, swimmer body size, and number of attached bacteria, toward an optimized design of such biohybrid system are studied. The optimized microswimmers would be used in targeted cargo, e.g., drug, imaging agent, gene, and RNA, transport and delivery inside the stagnant or low‐velocity fluids of the human body as one of their potential biomedical applications.

show abstract

Section: Introductionmentioning

confidence: 73%

Propulsion and Chemotaxis in Bacteria‐Driven Microswimmers

2017

View full text Add to dashboard Cite

show abstract

“…Instead, worms navigate a chemical gradient using temporal comparisons of encountered concentrations [73,74], similar to the mechanisms used in bacterial chemotaxis [75]. Worm movement on agar plates consists of periods of forward movement, punctuated by sudden turns or reversals that lead to changes in direction [76].…”

Section: Navigating Towards or Away From A Chemicalmentioning

confidence: 99%

Generation and modulation of chemosensory behaviors in C. elegans

Sengupta

2007

Pflugers Arch - Eur J Physiol

View full text Add to dashboard Cite

C. elegans recognizes and discriminates among hundreds of chemical cues using a relatively compact chemosensory nervous system. Chemosensory behaviors are also modulated by prior experience and contextual cues. Because of the facile genetics and genomics possible in this organism, C. elegans provides an excellent system in which to explore the generation of chemosensory behaviors from the level of a single gene to the motor output. This review summarizes the current knowledge on the molecular and neuronal substrates of chemosensory behaviors and chemosensory behavioral plasticity in C. elegans.

show abstract

“…Motion analysis involves reconstruction of bacterial paths from digitized video frames for quantitation of behavior. 22,23 This assay monitors chemokinetic rather than chemotactic activity as no gradient of attractant is established. It has the advan- tage that the behavior of individual cells can be followed rather than the bulk response of a population, and it is widely used to determine the response of bacteria to attractants and repellants.…”

Section: Resultsmentioning

confidence: 99%

Designed potent multivalent chemoattractants for Escherichia coli

Gestwicki

Strong

Borchardt

et al. 2001

Bioorganic &Amp; Medicinal Chemistry

View full text Add to dashboard Cite

Abstract-Bacterial chemotactic responses are initiated when certain small molecules (i.e., carbohydrates, amino acids) interact with bacterial chemoreceptors. Although bacterial chemotaxis has been the subject of intense investigations, few have explored the influence of attractant structure on signal generation and chemotaxis. Previously, we found that polymers bearing multiple copies of galactose interact with the chemoreceptor Trg via the periplasmic binding protein glucose/galactose binding protein (GGBP). These synthetic multivalent ligands were potent agonists of Escherichia coli chemotaxis. Here, we report on the development of a second generation of multivalent attractants that possess increased chemotactic activities. Strikingly, the new ligands can alter bacterial behavior at concentrations 10-fold lower than those required with the original displays; thus, they are some of the most potent synthetic chemoattractants known. The potency depends on the number of galactose moieties attached to the oligomer backbone and the length of the linker tethering these carbohydrates. Our investigations reveal the plasticity of GGBP; it can bind and mediate responses to several carbohydrates and carbohydrate derivatives. These attributes of GGBP may underlie the ability of bacteria to sense a variety of ligands with relatively few receptors. Our results provide insight into the design and development of compounds that can modulate bacterial chemotaxis and pathogenicity. #

show abstract

Chemotaxis in Escherichia coli analysed by Three-dimensional Tracking

Cited by 2,009 publications

References 9 publications

Propulsion and Chemotaxis in Bacteria‐Driven Microswimmers

Propulsion and Chemotaxis in Bacteria‐Driven Microswimmers

Generation and modulation of chemosensory behaviors in C. elegans

Designed potent multivalent chemoattractants for Escherichia coli

Contact Info

Product

Resources

About