Chemotactic methyltransferase promotes adaptation to repellents in Bacillus subtilis.

Kirsch, Michael L.; Zuberi, Aamir; Henner, Dennisj .; Peters, PJ; Yazdi, Mohammed A.; Ordal, George

doi:10.1016/s0021-9258(19)74398-1

Cited by 42 publications

(11 citation statements)

References 34 publications

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“…Fractions. Cell fractions were prepared according to Goldman and Ordal (1984) and Kirsch et al (1993b) with some modifications. Stationary phase cells were diluted 1:100 into 6 liters of LBr and were harvested after 10 h of incubation at 37 °C.…”

Section: Preparation Of Crude Soluble Extracts and Membranementioning

confidence: 99%

“…013154 was made by transformation of 013135 (cheC) with chromosomal DNA from 012680 (cheR); 012923 was made by transformation of Oil 100 (cheR) with linearized pWN2 (containing cheD: :cat). For methylesterase assays, membranes from a cheR-cheC mutant (013154) were prepared as described by Kirsch et al (1993b). These membrane preparations were labeled with S-adenosyl[me//iy/-3H]methionine using soluble extracts of 012714, an E. coli strain that produces B. subtilis cheR methyltransferase.…”

Section: Preparation Of Crude Soluble Extracts and Membranementioning

confidence: 99%

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Chemotactic methylation and behavior in Bacillus subtilis: role of two unique proteins, CheC and CheD

Rosario¹,

Kirby²,

Bochar³

et al. 1995

Biochemistry

101

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We characterized mutants in two novel genes of Bacillus subtilis, cheC and cheD. Mutants in CheC had a high smooth swimming bias and exhibited poor adaptation to positive stimuli. Analysis of tethered cells revealed two distinct subpopulations which differ in their prestimulus bias and extent of adaptation. The receptors, the methyl-accepting chemotaxis proteins (MCPs), of this mutant strain were overmethylated, as a result of an increase in CheR activity. We speculate that CheC helps to control tumbling frequency by regulating CheR, perhaps by a feedback mechanism through the MCPs. In contrast, a cheD mutant exhibited very tumbly behavior, and many of the MCPs were unmethylated. It seems that some B. subtilis MCPs require the presence of CheD for CheR to methylate them, a unique feature of B. subtilis chemotaxis. It is hypothesized that CheD is part of a complex that facilitates methylation of some of the MCPs, and dissociation of CheD from this complex affects CheA activity and may help bring about adaptation.

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Section: Preparation Of Crude Soluble Extracts and Membranementioning

confidence: 99%

Section: Preparation Of Crude Soluble Extracts and Membranementioning

confidence: 99%

Chemotactic methylation and behavior in Bacillus subtilis: role of two unique proteins, CheC and CheD

Rosario¹,

Kirby²,

Bochar³

et al. 1995

Biochemistry

101

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“…Receptor methylesterase; demethylates specific methyl-glutamate residues on the receptors [66,94,117,129,130,140,150,154,[161][162][163][164][165][166][167][168][169][170][171][172][173] x x CheC CheYp phosphatase; primary role is in adaptation via binding to CheD [45, 121, 122, 134-137, 139, 174-180] x…”

Section: Chebmentioning

confidence: 99%

“…Receptor deamidase; primary role is in adaptation via binding to CheC [121, 122, 134-139, 178, 179] x CheR Receptor methyltransferase; methylates specific glutamate resides on the receptors [55,125,127,140,143,164,170, x x CheV Couples CheA to receptors; response regulator domain can be phosphorylated; role in adaptation [27, 58-60, 94, 145, 178, 208] x CheW Couples CheA to the receptors [22, 39-42, 51, 54, 58, 59, 61, 67, 88, 209-231] x x…”

Section: Chedmentioning

confidence: 99%

“…The chemotaxis receptors in B. subtilis are also subject to reversible methylation by the CheR methyltransferase and CheB methylesterase. Furthermore, the basic mechanism is the same in both E. coli and B. subtilis, as both enzymes from B. subtilis can complement the corresponding E. coli null mutant [140,141]. Despite employing the same mechanism for adding and removing methyl groups at specific glutamate residues, the role of methylation in adaptation in B. subtilis is quite different than in E. coli.…”

Section: The Methylation Systemmentioning

confidence: 99%

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Bacterial Sensing and Signaling

Collin¹,

Schuch²

2009

Contributions to Microbiology

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Bibliographic Indices. This publication is listed in bibliographic services, including Current Contents® and Index Medicus.Disclaimer. The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publisher and the editor(s). The appearance of advertisements in the book is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.Drug Dosage. The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any change in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.

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A New Mathematical Approach Predicts Individual Cell Growth Behavior using Bacterial Population Information

Anderson

Mendelson

Watkins

2000

Journal of Theoretical Biology

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Chemotactic methyltransferase promotes adaptation to repellents in Bacillus subtilis.

Cited by 42 publications

References 34 publications

Chemotactic methylation and behavior in Bacillus subtilis: role of two unique proteins, CheC and CheD

Chemotactic methylation and behavior in Bacillus subtilis: role of two unique proteins, CheC and CheD

Bacterial Sensing and Signaling

A New Mathematical Approach Predicts Individual Cell Growth Behavior using Bacterial Population Information

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