Chemotactic factor-induced adherence of tumor cells

Varani, James; Wass, John; Piontek, Gerald E.; Ward, Peter A.

doi:10.1016/0309-1651(81)90180-6

Cited by 12 publications

(10 citation statements)

References 9 publications

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“…However, other types of studies show that the tumor cells respond very rapidly to stimulation with chemotactic factors. In both the cell swelling and foreign surface adherence assays, response to either the C5a-derived peptide or to FMLP can be detected within 1-3 min (16)(17)(18). This rapid response is similar to what is observed with leukocytes (12,13).…”

Section: Chemotactic Activation Of Tumor Cells In Vitrosupporting

confidence: 64%

“…This procedure differs from the first in that the cells are in contact with the substrate for a much longer period of time (up to 24 hr). Both procedures have been described in detail in our recent reports (18,19).…”

Section: Stimulated Adherence Of Tumor Cellsmentioning

confidence: 99%

“…These responses may all be relevant to the functioning of leukocytes in vivo. Likewise, functional responses other than motility also occur in stimulated tumor cells (16)(17)(18)(19). Of particular interest has been the finding that chemotactic factors induce a transient foreign surface adherence response in tumor cells (18,19).…”

Section: Introductionmentioning

confidence: 99%

“…Likewise, functional responses other than motility also occur in stimulated tumor cells (16)(17)(18)(19). Of particular interest has been the finding that chemotactic factors induce a transient foreign surface adherence response in tumor cells (18,19). This response may play a critical role in the process by which circulating tumor cells become localized at secondary sites in vivo.…”

Section: Introductionmentioning

confidence: 99%

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Themotaxis of metastatic tumor cells

Varani

1982

Cancer Metast Rev

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A number of factors have been identified which are chemotactic for tumor cells. Recent studies have shown that, in addition to inducing directional motility in the Boyden chamber assay, these factors also induce a number of other responses. Included among these responses are cell swelling and foreign surface adhesiveness. The adherence response has been studied in detail using the Walker 256 carcinosarcoma cells and several other cell types. In the Walker cells, treatment with the C5a-derived tumor cell chemotactic peptide, the synthetic tripeptide, N-formyl-methionyl-leucyl-phenylalanine or with 12-O-tetradecanoyl phorbol ester induces a rapid, transient adherence response. The response is completely inhibited by several agents known to block the activity of phospholipase A2 or the metabolism of arachidonic acid through the lipoxygenase pathway but is not inhibited by inhibition of the cyclooxygenase pathway. This suggests that lipoxygenase metabolites of arachidonic acid may actually mediate the adherence response. It has been shown that chemotactic factor treatment of animals that are bearing circulating tumor cells induces a localization of these cells at the site of chemotactic factor injection. On the basis of these observations it has been hypothesized that tumor cells respond to chemotactic factors in much the same way that leukocytes do and that tumor cell localization at metastatic sites in vivo may be influenced by chemotactic factors in much the same way that leukocyte localization at inflammatory sites is.

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Section: Chemotactic Activation Of Tumor Cells In Vitrosupporting

confidence: 64%

Section: Stimulated Adherence Of Tumor Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Themotaxis of metastatic tumor cells

Varani

1982

Cancer Metast Rev

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“…100 U penicillin/ml and 100 pg streptomycin/ml at 37 C in a humidified atmosphere containing 95% air and 5% C 0 2. These cells have been shown in previous studies to be responsive to phorbol esters in vitro and to chemotaetic peptides in vitro and in vivo [7][8][9][10]. Tissue culture reagents were purchased from Grand Island Biological Company (Grand Island.…”

Section: Methodsmentioning

confidence: 99%

Modulation of Tumor Cell Adherence by Prostaglandins

et al. 1983

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The initiation of tumor cell metastasis is thought to involve the adherence of circulating tumor cells to endothelial cells and/or basement membranes. The adherence of Walker carcinosarcoma tumor cells to foreign surfaces induced by phorbol myristate acetate and the chemotactic formyl peptide, formyl-methionyl-leucyl-phenylalanine is inhibited by prostacyclin and 15-S-15-methyl PGE₁ in a dose-dependent manner. The inhibition of tumor cell adherence by prostacyclin and 15-S-15-methyl PGE₁ is associated with a 3- to 5-fold increase in intracellular cyclic adenosine monophosphate (cAMP) concentrations. The data suggests that the local production of prostaglandins may be an important modulator of tumor cell metastasis due to its direct effect on the tumor cells.

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