Chemotactic and Phagocytic Activity of Blood Neutrophils in Allergic Asthma

Abstract

Allergic asthma is a chronic inflammatory airway disease, and has been considered a T helper-2-biased response. Studies suggest that neutrophils may be associated with exacerbation and asthma severity. We sought to evaluate the chemotactic activity and phagocytic capacity by peripheral blood neutrophils from individuals with controlled and uncontrolled allergic asthma, and compare the results with non-asthmatic controls groups. Blood neutrophils were isolated from 95 patients: 24 with controlled asthma, 24 uncontrolled asthma, 24 healthy subjects and 23 patients with IgE-mediated allergies other than asthma. The neutrophil chemotaxis, stimulated with LPS, autologous serum or homologous serum, was determined using Boyden chambers. The phagocytic capacity was assessed by ingestion of zimosan particles, and digestion phase was analyzed by NBT test. The phagocytic digestion phase and chemotaxis by neutrophils from asthmatic patients was higher than in non-asthmatic controls (p < 0.05). Autologous serum-induced neutrophil chemotaxis in patients with uncontrolled asthma was greater (p < 0.05) than in other study groups. The ingestion phase of phagocytosis showed similar values in asthmatics and non-asthmatics. We conclude that the blood neutrophil from controlled and uncontrolled asthmatic patients exhibit activation markers, particularly phagocytic digestion and chemotactic activities.

Advanced Role of Neutrophils in Common Respiratory Diseases

“…Blood neutrophils from allergic asthma also show the chemotactic and phagocytic activities towards LPS and asthmatic serum [43]. Asthmatics challenged with inhaled Dermatophagoides pteronyssinus (DP) would promote the production of neutrophil chemotaxis [44].…”

Aberrant function of neutrophils in asthma

“…Also, neutrophils from asthmatic patients exhibited increased chemotactic activity in the presence of homologous (pooled sera from healthy individuals) or autologous (patient's own serum) serum compared with non-asthmatic controls. Autologous serum-induced neutrophil chemotaxis in patients with uncontrolled asthma was greater than controlled asthma [13] . This phenomenon could be explained by increased chemotactic factors, such as IL-8, TNFα, LTB4 and IL-17A, in the circulation of patients suffering from asthma [6,[14][15][16][17] .…”

“…The effects of reactive oxygen species in asthma include decreased beta-adrenergic function in lungs, airway smooth muscle contraction, increased vascular permeability, bronchial hyperresponsiveness, increased mucus secretion, impaired ciliary activity, generation of chemotactic factors and lipid peroxidation, and secondary production of mediators with a bronchoconstrictor effect [27] . It has been demonstrated that neutrophils purified from bronchoalveolarlavage fluid and peripheral blood from patients with allergic asthma released significantly higher quantities of ROS compared to healthy individuals [13,19,[27][28][29] . In mice mode of asthma, the gp91phox deficiency mice, the dominant superoxide generating enzyme in neutrophils, had decreased ROS production, and attenuated allergic inflammatory response to allergen challenge [30] .…”

Alteración laboratorial que precede a las manifestaciones clínicas de infección estafilocócica postrasplante intestinal

“…4 Después de la separación de los neutrófilos por sedimentación espontánea 5 fueron utilizadas cámaras de Boyden y tubos de Leighton para evaluar la actividad quimiotáctica y de la etapa de ingestión de la fagocitosis por neutrófilos. 4,5 Tanto los exámenes de la paciente como los del grupo de control fueron realizados por duplicado.…”