Chemosystematic studies in the genus Picea (Pinaceae). III. The leaf oil of a Picea glauca × mariana hybrid (Rosendahl spruce)

Abstract

The terpene composition of the leaf oils of the Rosendahl spruce, white spruce, and black spruce as isolated from provenances derived from Cromwell, Minnesota, was determined by gas–liquid chromatography. The chemical composition was found to reflect the hybrid origin of the Rosendahl spruce. This confirms that the leaf oil composition of Picea species may serve as a biochemical character in chemo systematic studies.

Chromatographic Analysis of an Open-pollinated Rosendahl Spruce Progeny

“…The chemical analysis used in this study provided support for the morphological evi dence presented by Little and Pauley (1958) and studies of terpene composition of leaf oils by von Rudloff and Holst (1968) The data suggested hybridization and subse-quent introgression could have contributed to the genetic variation . of both our native spruces.…”

Successful colonization, reproduction, and new generation emergence in live interior hybrid spruce Picea engelmannii×glauca by mountain pine beetle Dendroctonus ponderosae

“…hosts (Amman, et al , 1990). Although the different hosts are probably quite similar in terms of chemical defences (von Rudloff & Holst, 1968; Hunt & von Rudloff 1977; von Rudloff & Lapp, 1987), there are differences in quality and quantity of secondary metabolites among host species and even between populations of the same host species (Forrest, 1980). As such, it is likely that a coevolutionary mosaic exists across the range of this insect, with interactions between mountain pine beetle and its various hosts in some regions causing specific changes in the ability of the beetles to resist certain tree defences (Raffa & Berryman, 1987; Thompson, 1999).…”

“…Thus, because the terpenoid secondary metabolite defensive secretions of some Pinus spp. hosts (Huber et al , 2004) of mountain pine beetle are at least similar in quality to those of interior hybrid spruce (von Rudloff & Holst, 1968; Hunt & von Rudloff, 1977; von Rudloff & Lapp, 1987; Pureswaran et al , 2004), the possibility of pre‐adaptation of some mountain pine beetle populations to some phenotypes of spruce exists. Some mountain pine beetle may then utilize spruce when they grow in close proximity to normal hosts, as is the case in central British Columbia in mixed stands of lodgepole pine and interior hybrid spruce.…”

Gas-liquid chromatography of terpenes. Part XVI. The volatile oil of the leaves of Juniperus ashei Buchholz

“…The distillate was extracted with methylene chloride and, after drying, the solvent was removed by distillation; yield 1.1 %, [a]i2 f 37.3" (c, 12.7; CHCI,), rzi2 1.4687. Leaf samples of 15 to 30 g were sent in polyethylene bags from Austin and small scale distillation was carried out as described earlier (12). During the steam-distillation camphor, m.p.…”

“…was described earlier (7,8,11). However, it has since become apparent that quantitative differences may be significant in studies of introgression or hybridization (5,12). Hence, improvement of the quantitative results would be desirable as determination of peak areas by the triangulation method or similar techniques can be subject to comparatively large errors (13), especially in partially resolved or minor peaks.…”