Chemosensory-Related Gene Family Members of the Horn Fly, Haematobia irritans irritans (Diptera: Muscidae), Identified by Transcriptome Analysis

Olafson, Pia U.; Saski, Christopher

doi:10.3390/insects11110816

Cited by 6 publications

(3 citation statements)

References 89 publications

(117 reference statements)

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“…House fly Obp genes can be grouped into families corresponding to their D. melanogaster orthologs (Scott et al 2014). The DE Obp gene in our analysis is orthologous to Obp56h , The Obp56h family, as well as other Obp families, was greatly expanded within muscids (house fly and close relatives, including stable fly and horn fly) compared to D. melanogaster (Scott et al2014; Olafson and Saski 2020; Olafson et al 2021). In addition to LOC105261916 , seven of the remaining eight house fly Obp56h genes for which we obtained RNA-seq count data showed similar trends of greater expression in Y M than III M males, with three of these showing significant DE ( p <0.05) before an FDR correction (Fig.…”

Section: Resultsmentioning

confidence: 63%

Transregulation of an odorant binding protein by a proto-Y chromosome affects male courtship in house fly

Adhikari

Hassan

et al. 2021

Preprint

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A major goal in evolutionary biology is to understand how natural variation is maintained in sexually selected and sexually dimorphic traits. Hypotheses to explain genetic variation in sexually selected traits include context-dependent fitness effects, epistatic interactions, and pleiotropic constraints. The house fly, Musca domestica, is a promising system to investigate how these factors affect polymorphism in sexually selected traits. Two common Y chromosomes (YM and IIIM) segregate as stable polymorphisms in natural house fly populations, appear to be locally adapted to different thermal habitats, and differentially affect male mating success. Here, we perform a meta-analysis of RNA-seq data which identifies genes encoding odorant binding proteins (in the Obp56h family) as differentially expressed between the heads of males carrying YM and IIIM. Differential expression of Obp56h has been associated with variation in male mating behavior in Drosophila melanogaster. We find differences in male mating behavior between house flies carrying the Y chromosomes that are consistent with the relationship between male mating behavior and expression of Obp56h in D. melanogaster. We also find that male mating behaviors in house fly are affected by temperature, and the same temperature differentials further affect the expression of Obp56h genes. However, we show that temperature-dependent effects cannot explain the maintenance of genetic variation for male mating behavior in house fly. Using a network analysis and allele-specific expression measurements, we find evidence that the house fly IIIM chromosome is a trans regulator of Obp56h gene expression. Moreover, we find that Obp56h disproportionately affects the expression of genes on the D. melanogaster chromosome that is homologous to the house fly IIIM chromosome. This provides evidence for a conserved trans regulatory loop involving Obp56h expression that affects male mating behavior in flies. The complex regulatory architecture controlling Obp56h expression suggests that variation in male mating behavior could be maintained by epistasis or pleiotropic constraints.

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Section: Resultsmentioning

confidence: 63%

Transregulation of an odorant binding protein by a proto-Y chromosome affects male courtship in house fly

Adhikari

Hassan

et al. 2021

Preprint

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“…For some years now, progress has been made in the study of the olfactory system of several insect vectors of human diseases (see for example [ 35 ]). Moreover, new transcriptomic analysis techniques have made it possible to study the olfactory system of many other pest insects (see for example [ 36 , 37 ]). These types of studies could allow the discovery of species-specific components of their olfactory systems, such as odorant receptors or odorant-binding proteins (OBPs) that could be exploited to manipulate olfactory behavior in specific insects.…”

Section: Resultsmentioning

confidence: 99%

Validation of an Optogenetic Approach to the Study of Olfactory Behavior in the T-Maze of Drosophila melanogaster Adults

Coya

Martín

Calvin-Cejudo

et al. 2022

Insects

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Optogenetics enables the alteration of neural activity using genetically targeted expression of light activated proteins for studying behavioral circuits in several species including Drosophila. The main idea behind this approach is to replace the native behavioral stimulus by the light-induced electrical activation of different points of the circuit. Therefore, its effects on subsequent steps of the circuit or on the final behavior can be analyzed. However, the use of optogenetics to dissect the receptor elements of the adult olfactory behavior presents a challenge due to one additional factor: Most odorants elicit attraction or avoidance depending on their concentration; this complicates the representative replacement of odor activation of olfactory sensory neurons (OSNs) by light. Here, we explore a dual excitation model where the subject is responding to odors while the OSNs are optogenetically activated. Thereby, we can assess if and how the olfactory behavior is modified. We measure the effects of light excitation on the response to several odorant concentrations. The dose-response curve of these flies still depends on odor concentration but with reduced sensitivity compared to olfactory stimulation alone. These results are consistent with behavioral tests performed with a background odor and suggest an additive effect of light and odor excitation on OSNs.

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“…gambiae [ 23 ], Ae. aegypti [ 66 ] and other insects [ 18 , 67 , 68 ]. The differences in expression profiles of chemosensory genes strongly suggested that the odor coding of antennae is far more complex and stronger than that of the maxillary palp or proboscis.…”

Section: Resultsmentioning

confidence: 99%

Genome-wide identification and expression profiling of odorant receptor genes in the malaria vector Anopheles sinensis

et al. 2022

Parasites Vectors

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Background The olfactory system plays a crucial role in regulating insect behaviors. The detection of odorants is mainly mediated by various odorant receptors (ORs) that are expressed in the dendrites of olfactory neurons of chemosensilla. Anophelessinensis is a major malaria vector in Eastern Asia and its genome has recently been successfully sequenced and annotated. In this study, we present genome-wide identification and expression profiling of OR genes in different chemosensory tissues of An.sinensis. Methods The OR genes were identified using the available genome sequences of An.sinensis. A series of bioinformatics analyses were conducted to investigate the structure, genome distribution, selective pressure and phylogenetic relationships of OR genes, the conserved domains and specific functional sites in the OR amino acid sequences. The expression levels of OR genes were analyzed from transcriptomic data from An.sinensis antennae, proboscis and maxillary palps of both sexes. Results A total of 59 putative OR genes have been identified and characterized in An.sinensis. This number is significantly less than that in An.gambiae. Whether this difference is caused by the contraction or expansion of OR genes after divergence of the two species remains unknown. The RNA-seq analysis showed that AsORs have obvious tissue- and sex-specific expression patterns. Most AsORs are highly expressed in the antennae and the expression pattern and number of AsORs expressed in antennae are similar in males and females. However, the relative levels of AsOR transcripts are much higher in female antennae than in male antennae, which indicates that the odor sensitivity is likely to be increased in female mosquitoes. Based on the expression patterns and previous studies, we have speculated on the functions of some OR genes but this needs to be validated by further behavioral, molecular and electrophysiological studies. Further studies are necessary to compare the olfactory-driven behaviors and identify receptors that respond strongly to components of human odors that may act in the process of human recognition. Conclusions This is the first genome-wide analysis of the entire repertoire of OR genes in An.sinensis. Characterized features and profiled expression patterns of ORs suggest their involvement in the odorous reception of this species. Our findings provide a basis for further research on the functions of OR genes and additional genetic and behavioral targets for more sustainable management of An.sinensis in the future. Graphical Abstract

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Chemosensory-Related Gene Family Members of the Horn Fly, Haematobia irritans irritans (Diptera: Muscidae), Identified by Transcriptome Analysis

Cited by 6 publications

References 89 publications

Transregulation of an odorant binding protein by a proto-Y chromosome affects male courtship in house fly

Transregulation of an odorant binding protein by a proto-Y chromosome affects male courtship in house fly

Validation of an Optogenetic Approach to the Study of Olfactory Behavior in the T-Maze of Drosophila melanogaster Adults

Genome-wide identification and expression profiling of odorant receptor genes in the malaria vector Anopheles sinensis

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