2022
DOI: 10.3389/fevo.2021.795994
|View full text |Cite
|
Sign up to set email alerts
|

Chemosensory Receptors in the Larval Maxilla of Papilio hospiton

Abstract: Among the butterflies of the genus Papilio (Lepidoptera: Papilionidae), Papilio hospiton (Géné) has a geographical distribution limited to the Mediterranean islands of Sardinia (Italy) and Corsica (France). This is mainly due to the host range that includes only a few plant species of Apiaceae and Rutaceae growing on these islands. In a previous electrophysiological investigation conducted on the maxillary gustatory system of larvae of P. hospiton and its closely phylogenetically related species Papilio machao… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
2
1

Citation Types

0
12
0

Year Published

2022
2022
2024
2024

Publication Types

Select...
3
2

Relationship

3
2

Authors

Journals

citations
Cited by 8 publications
(12 citation statements)
references
References 95 publications
0
12
0
Order By: Relevance
“…In the last decades, most of the investigation of lepidopterans targeted their chemoreceptors [ 49 , 50 , 51 , 52 ]. Among various reports, some provided in-depth analysis of specific OBPs [ 53 , 54 ], CSPs [ 18 , 55 ], or both [ 30 , 56 ]. However, to our knowledge, this is among the few [ 57 ], or may be the sole study in lepidopterans, in which a systematic investigation of soluble proteins merges with a variegate analysis including genomic loci, gene and polypeptide structures, expression patterns across every developmental stage, and functional evidence of semiochemical/pesticide binding.…”
Section: Discussionmentioning
confidence: 99%
“…In the last decades, most of the investigation of lepidopterans targeted their chemoreceptors [ 49 , 50 , 51 , 52 ]. Among various reports, some provided in-depth analysis of specific OBPs [ 53 , 54 ], CSPs [ 18 , 55 ], or both [ 30 , 56 ]. However, to our knowledge, this is among the few [ 57 ], or may be the sole study in lepidopterans, in which a systematic investigation of soluble proteins merges with a variegate analysis including genomic loci, gene and polypeptide structures, expression patterns across every developmental stage, and functional evidence of semiochemical/pesticide binding.…”
Section: Discussionmentioning
confidence: 99%
“…In this work, we also explored the heterologous expression in HEK cells, which is a single-cell ex vivo system such as Xenopus oocytes. HEK cells have been largely used in the deorphanization of ORs (Cattaneo et al, 2017; Bobkov et al, 2021, Crava et al, 2022, Miazzi et al, 2019, Grosse-Wilde et al, 2007; 2006) whereas, to our knowledge, this method has not been previously utilized for IR subunit deorphanization. We conducted several experiments using this approach but did not achieve successful deorphanization of IRs, despite observing correct expression of IRs in transfected cells through immunohistochemistry experiments (Figure 5).…”
Section: Discussionmentioning
confidence: 99%
“…The full-length coding sequences of C. pomonella IR41a1, IR25a and IR76b and of D. suzukii IR8a were obtained from previous studies (Walker et al, 2016; Walker et al, 2023; Ramasamy et al, 2016). Sequences were submitted to GeneArt TM gene synthesis service (Thermos Fischer Scientific, Waltham, MA, USA) requesting integration of the 5’-upstram CACC Kozak-sequence, as done previously (Bobkov et al, 2021; Cattaneo et al, 2017; Crava et al, 2022), codon optimization for expression in human cells (Roberts et al, 2021) and integration into pcDNA3.1 plasmids. To test expression of D. melanogaster IR8a+IR84a, the Gibson-assembled combination of these two CDS into pcDNA3.1 was courtesy provided by Dr. Hayden R. Schmidt (International AIDS Vaccine Initiative, USA).…”
Section: Methodsmentioning
confidence: 99%
See 2 more Smart Citations