Chemosensory mechanisms of host seeking and infectivity in skin-penetrating nematodes

Gang, Spencer S.; Castelletto, Michelle L.; Yang, Emily; Ruiz, Felicitas; Brown, Taylor M; Bryant, Astra S.; Grant, Warwick N.; Hallem, Elissa A.

doi:10.1073/pnas.1909710117

Cited by 45 publications

(60 citation statements)

References 71 publications

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“…For example, S. stercoralis iL3s respond robustly to a number of human-associated odorants, and have distinct olfactory preferences from those of other life stages (Castelletto et al . 2014; Gang et al . 2020).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Strongyloides RNA-seq Browser: a web-based software platform for on-demand bioinformatics analyses of Strongyloides species

Bryant¹,

DeMarco²,

Hallem³

2021

Preprint

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Soil-transmitted gastrointestinal parasitic nematodes infect approximately 1 billion people worldwide, predominantly in low-resource communities. Skin-penetrating gastrointestinal nematodes in the genus Strongyloides are emerging as model systems for mechanistic studies of soil-transmitted helminths due to the growing availability of functional genomics tools for these species. To facilitate future genomics studies of Strongyloides species, we have designed a web-based application, the Strongyloides RNA-seq Browser, that provides an open source, user-friendly portal for accessing and analyzing Strongyloides genomic expression data. Specifically, the Strongyloides RNA-seq Browser takes advantage of alignment-free read mapping tools and R-based transcriptomics tools to re-analyze publicly available RNA sequencing datasets from four Strongyloides species: Strongyloides stercoralis, Strongyloides ratti, Strongyloides papillosus, and Strongyloides venezuelensis. This application permits on-demand exploration and quantification of gene expression across life stages without requiring previous coding experience. Here, we describe this interactive application and demonstrate how it may be used by nematode researchers to conduct a standard set of bioinformatics queries.

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Section: Resultsmentioning

confidence: 99%

“…Interestingly, although most GPCRs are downregulated in non-iL3 life stages, S. stercoralis free-living adults display robust chemosensory behaviors, including broad attraction to host-associated odorants (Castelletto et al . 2014; Gang et al . 2020).…”

Section: Resultsmentioning

confidence: 99%

Strongyloides RNA-seq Browser: a web-based software platform for on-demand bioinformatics analyses of Strongyloides species

Bryant¹,

DeMarco²,

Hallem³

2021

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Currently, genome editing in nematodes is mostly limited to Caenorhabditis species. Some work in genome editing in parasite species has been performed successfully in Strongyloides spp [78][79][80][81][82][83][84]. When these technologies are further optimized and can be performed in H. contortus, the cycle of discovery can be greatly improved and expanded.…”

Section: Genome Editing In H Contortusmentioning

confidence: 99%

Complementary Approaches to Understand Anthelmintic Resistance Using Free-Living and Parasitic Nematodes

J¹,

Dilks²,

Andersen³

2020

Preprint

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Anthelmintic drugs are the major line of defense against parasitic nematode infections, but the arsenal is limited and resistance threatens sustained efficacy of the available drugs. Discoveries of the modes of action of these drugs and mechanisms of resistance have predominantly come from studies of a related non-parasitic nematode species, Caenorhabditis elegans, and the parasitic nematode Haemonchus contortus. Here, we discuss how our understanding of anthelmintic resistance and modes of action came from the interplay of results from each of these species. We argue that this “cycle of discovery”, where results from one species inform the design of experiments in the other, can use the complementary strengths of both to understand anthelmintic modes of action and mechanisms of resistance.

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“…Despite these difficulties, genome editing has been performed successfully in Strongyloides spp. because the life cycle is amenable to laboratory manipulation [114][115][116][117][118][119][120]. For other parasitic nematode species, the cycle of discovery between parasitic and free-living nematodes offers promise but genome editing might still be in the distant future.…”

Section: Lack Of Widespread Genome Editing In Parasitic Nematodesmentioning

confidence: 99%

Complementary Approaches to Understand Anthelmintic Resistance Using Free-Living and Parasitic Nematodes

Wit¹,

Dilks²,

Andersen³

2020

Preprint

View full text Add to dashboard Cite

Parasitic nematode infections impact human and animal health globally, especially in the developing world. Anthelmintic drugs are the major line of defense against these infections, but the arsenal is limited. Additionally, anthelmintic resistance is widespread in veterinary parasites and an emerging threat in human parasites. Discoveries of the mode of action of these drugs and mechanisms of resistance have predominantly come from studies of a related non-parasitic nematode species, Caenorhabditis elegans, and the parasitic nematode Haemonchus contortus. Here, we discuss recent progress understanding anthelmintic resistance using these two species and how that progress relates to laboratory and field-based studies of veterinary helminths. We present a powerful approach enabled by the strengths of both nematode species to understand mechanisms of resistance and modes of action of anthelmintic drugs.

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Chemosensory mechanisms of host seeking and infectivity in skin-penetrating nematodes

Cited by 45 publications

References 71 publications

Strongyloides RNA-seq Browser: a web-based software platform for on-demand bioinformatics analyses of Strongyloides species

Strongyloides RNA-seq Browser: a web-based software platform for on-demand bioinformatics analyses of Strongyloides species

Complementary Approaches to Understand Anthelmintic Resistance Using Free-Living and Parasitic Nematodes

Complementary Approaches to Understand Anthelmintic Resistance Using Free-Living and Parasitic Nematodes

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