Chemosensitizing activity of Cuban propolis and nemorosone in doxorubicin resistant human colon carcinoma cells

Frión-Herrera, Yahima; Gabbia, Daniela; Díaz-García, Alexis; Cuesta‐Rubio, Osmany; Carrara, Maria

doi:10.1016/j.fitote.2019.104173

Cited by 26 publications

(25 citation statements)

References 39 publications

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“…This study clearly showed that brown Cuban propolis and NEM displayed a pronounced inhibitory effect on the proliferation of HT-29 and LoVo cells. Furthermore, according to others reports [32,36,38], considering the NEM content of CP and the IC 50 values obtained in this study, we further confirmed that the biological activity of CP is strictly dependent on its nemorosone content.…”

Section: Discussionsupporting

confidence: 92%

“…Their effect on HT-29 and LoVo viability was supported by the results of the clonogenic assay, which showed a significant decrease of the colony-forming capacity of CP-and NEM-treated cells compared to untreated cells, further confirming their antiproliferative effect. We have previously reported that CP and NEM have an inhibitory effect on LoVo cell viability [32], thus the results of the present study confirmed this finding and extended it to another CRC cell line. On the other hand, we observed differences in the susceptibility of these two CRC cell lines after 10 days of treatment with NEM, which was more active against LoVo than HT-29 when used at the lowest concentration.…”

Section: Discussionsupporting

confidence: 90%

“…However, no significant differences were observed between the two CRC cell lines regarding the IC 50 values (Table 1), thus indicating that HT-29 and LoVo cells have a similar susceptibility to NEM and CP effects. As already demonstrated by previous studies ( [32] and refs. therein), considering NEM content in CP, the IC 50 obtained in this study confirm that the antiproliferative effect of CP on CRC cell lines is mainly due to NEM.…”

Section: Nem and Cp Suppress Crc Cell Viabilitysupporting

confidence: 87%

“…The therapeutic effects of propolis depend on its chemical composition, which is related to the geographical area of origin and the environmental conditions of its production [27][28][29]. Several studies have shown that brown Cuban propolis (CP) exerts antiproliferative and cytotoxic properties on different cancer cell lines [30][31][32]. The chemical profiling of this type of propolis indicated that nemorosone (NEM), a prenylated benzophenone, is the main component of brown CP [33][34][35] to which the biological properties of this propolis can be attributed [36][37][38].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The Cuban Propolis Component Nemorosone Inhibits Proliferation and Metastatic Properties of Human Colorectal Cancer Cells

Frión-Herrera

Gabbia

Scaffidi

et al. 2020

IJMS

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The majority of deaths related to colorectal cancer (CRC) are associated with the metastatic process. Alternative therapeutic strategies, such as traditional folk remedies, deserve attention for their potential ability to attenuate the invasiveness of CRC cells. The aim of this study is to investigate the biological activity of brown Cuban propolis (CP) and its main component nemorosone (NEM) and to describe the molecular mechanism(s) by which they inhibit proliferation and metastatic potential of 2 CRC cell lines, i.e., HT-29 and LoVo. Our results show that CP and NEM significantly decreased cell viability and inhibited clonogenic capacity of CRC cells in a dose and time-dependent manner, by arresting the cell cycle in the G0/G1 phase and inducing apoptosis. Furthermore, CP and NEM downregulated BCL2 gene expression and upregulated the expression of the proapoptotic genes TP53 and BAX, with a consequent activation of caspase 3/7. They also attenuated cell migration and invasion by inhibiting MMP9 activity, increasing E-cadherin and decreasing β-catenin and vimentin expression, proteins involved in the epithelial–mesenchymal transition (EMT). In conclusion NEM, besides displaying antiproliferative activity on CRC cells, is able to decrease their metastatic potential by modulating EMT-related molecules. These finding provide new insight about the mechanism(s) of the antitumoral properties of CP, due to NEM content.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 90%

Section: Nem and Cp Suppress Crc Cell Viabilitysupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Cuban Propolis Component Nemorosone Inhibits Proliferation and Metastatic Properties of Human Colorectal Cancer Cells

Frión-Herrera

Gabbia

Scaffidi

et al. 2020

IJMS

Self Cite

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“…Table 2 describes the viability percentages using 50 μg/mL of the different G. neuberthii extracts or Dxo 0.3 μg/mL on the human tumor cell lines after 48 h of treatment. Doxorubicin, an anthracycline antibiotic, has proved to induce cytotoxicity, cell cycle arrest, and apoptosis in a wide variety of tumor cell lines, including colon cancer cells; however, its clinical use is limited due its cardiotoxicity effect [16][17][18]. Cell growth viability was measured using the MTS assay, considering the control as 100% of viability.…”

Section: Cytotoxic Effect On Human Tumor Cell Linesmentioning

confidence: 99%

Cytotoxic Property of Grias neuberthii Extract on Human Colon Cancer Cells: A Crucial Role of Autophagy

Guamán-Ortiz

Romero-Benavides

Suárez

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Traditional herbal medicine has become an important alternative in the treatment of various cancer types, including colon cancer, which represents one of the main health problems around the world. Therefore, the search for new therapies to counteract this disease is very active. Grias neuberthii is an endemic plant located in the Ecuadorian Amazon region, which has been used in traditional medicine for its pharmacological properties, including its ability to inhibit tumor cell growth, although scientific studies are limited. We have analyzed the effect of this plant on two colon carcinoma cell lines, that is, RKO (normal p53) and SW613-B3 (mutated p53) cells. Among several extracts obtained from various parts of G. neuberthii plant, we identified the extract with the greatest cytotoxic potential, derived from the stem bark. The cytotoxic effect was similar on both cell lines, thus indicating that it is independent of the status of p53. However, significant differences were observed after the analysis of colony formation, with RKO cells being more sensitive than SW613-B3. No evidence for apoptotic markers was recorded; nevertheless, both cell lines showed signs of autophagy after the treatment, including increased Beclin-1 and LC3-II and decreased p62. Finally, three chemical compounds, possibly responsible for the effect observed in both cell lines, were identified: lupeol (1), 3′-O-methyl ellagic acid 4-O-β-D-rhamnopyranoside (2), and 19-α-hydroxy-asiatic acid monoglucoside (3).

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Ginsenoside RG1 augments doxorubicin‐induced apoptotic cell death in MDA‐MB‐231 breast cancer cell lines

Liu

Huang

Gao

et al. 2021

J Biochem & Molecular Tox

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This study determined the chemosensitizing potential of ginsenoside Rg1 in triplenegative MDA-MB-231 breast cancer cell lines. Ginsenoside Rg1 (10 µM) treated breast cancer cells were exposed to 8 nM of doxorubicin, and the chemosensitizing potential was measured by cell-based assays. Ginsenoside Rg1 (10 µM) treatment lowered the doxorubicin IC 50 value to 0.01 nM. Furthermore, the ginsenoside pretreatment augments doxorubicin-mediated reactive oxygen species (ROS) generation and subsequent alterations of mitochondrial membrane potential in MDA-MB-231 cell lines. The alkaline comet assay results illustrated an increased % tail DNA during ginsenoside Rg1 plus doxorubicin treatment than doxorubicin alone treatment. In addition, the number of apoptotic cells was also increased in ginsenoside Rg1 plus doxorubicin-treated cells. Furthermore, the polymerase chain reaction array results illustrate activation of mitogen-activated protein kinase (MAPK) gene expression (AKT, ERK, and MAPK) during doxorubicin alone treatment and it has been attenuated by ginsenoside Rg1 pretreatment. Moreover, ginsenoside Rg1 treatment before doxorubicin activates the DNA damage response elements (ATM, H2AX, RAD51, and XRCC1) and subsequent apoptosis-related gene expression (p21, TP53. APAF1, Bax, CASP3, and CASP9) patterns in MDA-MB-231 cell lines. The ginsenoside Rg1 plus doxorubicin combination shows less cytotoxicity and ROS generation in MDA10A normal breast cancer cell lines. Therefore, the present results support the chemosensitizing property of ginsenoside Rg1 in triple-negative breast cancer cell lines.

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Chemosensitizing activity of Cuban propolis and nemorosone in doxorubicin resistant human colon carcinoma cells

Cited by 26 publications

References 39 publications

The Cuban Propolis Component Nemorosone Inhibits Proliferation and Metastatic Properties of Human Colorectal Cancer Cells

The Cuban Propolis Component Nemorosone Inhibits Proliferation and Metastatic Properties of Human Colorectal Cancer Cells

Cytotoxic Property of Grias neuberthii Extract on Human Colon Cancer Cells: A Crucial Role of Autophagy

Ginsenoside RG1 augments doxorubicin‐induced apoptotic cell death in MDA‐MB‐231 breast cancer cell lines

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