Chemosensitivity testing of human gliomas using a fluorescent microcarrier technique

Abstract

This report describes a new fluorescent microcarrier cytostasis assay. Human glioma cell lines and primary cultures were attached to microcarrier tissue culture beads and treated with various chemotherapeutic drugs. After treatment, the cells were labelled with two vital fluorescent dyes in order to measure cellular viability. The uptake of hydroethidine and Hoechst 33342 was evaluated alone and in combination as probes for determining metabolic activity and cellular proliferation. Hydroethidine was found to be superior when compared to trypan blue and tritiated thymidine. The use of the microcarrier technique allows for the direct cellular measurement of fluorescence without the need of extensive extraction procedures. The fluorescent assay is a sensitive, rapid and an effective way to screen for potential antiproliferative compounds.

Hydroethidine- and MitoSOX-derived red fluorescence is not a reliable indicator of intracellular superoxide formation: Another inconvenient truth

“…Moreover, the extent of HE uptake and accumulation may be dependent not only on the dye concentration and incubation time, but also on the metabolic state of the cells [19]. HE was used in the investigation of antiproliferative compounds [24-26] and in other biological studies as a vital stain [27-31]. …”

Chemosensitivity of glioma cells in vitro: a meta analysis

“…As shown in Fig. 1, C6 was the Sariban et al 1987HTSCA 10 Panasci et al 1985Bowles et al 1990;Nikkhah et al 1992;Jordan et al 1992 [ 35 Results of primary cultures were within the range of the results for dierent long-term-cultured cell lines. Comparing the agents showed that the rank of ecacy within the same cell line varied less than in dierent cell lines.…”