Chemoresistenz des primären Nierenzellkarzinoms im In-vitro-Kurzzeittest und ihre Durchbrechung mit Verapamil

Mickisch, G.; Volm, M; Tschada, R.; Potempa, Jan; Alken, P.

doi:10.1055/s-2008-1061192

Cited by 3 publications

(3 citation statements)

References 16 publications

(20 reference statements)

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“…1. Determination of the degree of chemoresistance in a 3H-uridine uptake assay [10]. □ = Highly resistant (n = 17); ■ = less resistant (n = 4; average ± SD).…”

Section: Resultsmentioning

confidence: 99%

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P-170 Glycoprotein, Glutathione and Associated Enzymes in Relation to Chemoresistance of Primary Human Renal Cell Carcinomas

Mickisch¹,

Bier

Bergler

et al. 1990

Urol Int

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High intrinsic chemoresistance contributes to the dismal outcome of patients with disseminated renal cell carcinoma (RCC). In experimental cell lines, two defined defence mechanisms, P-170 glycoprotein and glutathione metabolism, have been established in multidrug resistance, a cross-resistance to cytotoxic compounds without functional or structural similarities. In 21 primary human RCCs, P-170 expression was examined, glutathione content and activities of related enzymes determined and the results were correlated to the degree of in vitro chemoresistance. P-170 was found in 10 of 17 resistant tumors but in none of the sensitive cases. The glutathione content was significantly higher and the related enzyme distinctively enhanced in resistant RCCs. Both mechanisms occurred independently and may well explain the multidrug resistance of RCC. Therefore, reversal of one or both systems may have a clinical impact on the chemotherapy of RCCs.

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“…1. Determination of the degree of chemoresistance in a 3H-uridine uptake assay [10]. □ = Highly resistant (n = 17); ■ = less resistant (n = 4; average ± SD).…”

Section: Resultsmentioning

confidence: 99%

“…The adaptation of the 'H-uridine uptake assay for primary RCCs has been previously described [10]. The basic feature is the inhibition of radioactive nucleic acid precursor uptake in the pres ence of cytotoxic compounds.…”

Section: In Vitro Short-term Testmentioning

confidence: 99%

P-170 Glycoprotein, Glutathione and Associated Enzymes in Relation to Chemoresistance of Primary Human Renal Cell Carcinomas

Mickisch¹,

Bier

Bergler

et al. 1990

Urol Int

Self Cite

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“…Versuche, die Behandlungsaussichten durch Einführung neuer Chemotherapeutika oder durch die Kombination verschiedener Medikamente mit unterschiedlicher antineoplastischer Wirkweise zu steigern, haben sich als nutzlos erwiesen (31). (18) als auch Vinblastin-Resistenz (20) durch Verapamil in vitro überwunden werden kann. Ziel dieser Studie ist es nun zu klären, welche Einzelsubstanz aus der Klasse der Kalziumantagonisten sich für weitere Untersuchungen im Hinblick auf eine klinische Anwendung beim Hypernephrom anbietet.…”

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