Chemoprotective effects of hesperidin against genotoxicity induced by cyclophosphamide in mice bone marrow cells

Ahmadi, Amirhossein; Hosseinimehr, Seyed Jalal; Naghshvar, Farshad; Hajir, Ebrahim; Ghahremani, Mehran

doi:10.1007/s12272-001-1228-z

Cited by 73 publications

(62 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As result, both drugs are genotoxic agents because they and their metabolites can bind DNA, causing damage that may result in chromosome breaks, micronucleus formation and cell death (Ahmadi et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Antigenotoxicity protection of Carapa guianensis oil against mitomycin C and cyclophosphamide in mouse bone marrow

Lemes

Chaves

Silva

et al. 2017

An. Acad. Bras. Ciênc.

View full text Add to dashboard Cite

The aim of this study was to evaluate the possible protective of C. guianensis oil against MMC and CP, which are direct-and indirect-acting chemical mutagens, using the micronucleus test. Three experiments were performed. First the C. guianensis oil was co-administered to mice at doses of 250, 500 and 1000 mg/ kg bw with 4 mg/kg bw MMC or 50 mg/kg bw CP. Second, the mutagenic drug (CP) was administered ip 50 mg/kg bw and after 6 and 12 hours 250 and 500 mg/kg bw of C. guianensis oil were administered. In the last, C. guianensis oil was administrated (250 and 500 mg/kg bw) during five days and after it was administered ip 50 mg/kg bw CP. The results obtained showed that the C. guianensis oil is not cytotoxic neither genotoxic to mouse bone marrow. Regarding the antimutagenic effect, all doses of C. guianensis oil were significantly (p < 0.05) effective in reducing the frequency of micronucleated polychromatic erythrocytes, when compared with MMC or CP alone. Based on these results, our results suggest that the C. guianensis oil shows medicinal potential as an antimutagenic agent, modulating the mutagenicity caused by both direct-and indirect-acting chemical mutagens, in a mammalian model.

show abstract

Section: Discussionmentioning

confidence: 99%

Antigenotoxicity protection of Carapa guianensis oil against mitomycin C and cyclophosphamide in mouse bone marrow

Lemes

Chaves

Silva

et al. 2017

An. Acad. Bras. Ciênc.

View full text Add to dashboard Cite

show abstract

“…Previous studies have shown that CP alters sperm chromatin structure, composition of sperm head basic proteins and increases abnormal sperm rate, and manifest biochemical and histological alterations in testis [2][3][4]. It has been reported that oxidative stress-induced biochemical and physiological damage is responsible for CP toxicity in testis and spermatozoa [5][6][7]. The mitochondrial membrane of spermatozoa is more susceptible to lipid peroxidation, as this compartment is rich in polyunsaturated fatty acids and has been shown to contain low amounts of antioxidants [8,9].…”

mentioning

confidence: 99%

Toxic Effect of Cyclophosphamide on Sperm Morphology, Testicular Histology and Blood Oxidant‐Antioxidant Balance, and Protective Roles of Lycopene and Ellagic Acid

Çeribaşı

Türk

Sönmez

et al. 2010

Basic Clin Pharma Tox

View full text Add to dashboard Cite

In this study, the toxic effect of cyclophosphamide (CP) on sperm morphology, testicular histology and blood oxidant-antioxidant balance, and protective roles of lycopene (LC) and ellagic acid (EA) were investigated. For this purpose, 48 healthy, adult, male Sprague-Dawley rats were divided into six groups; eight animals in each group. The control group was treated with placebo. LC, EA and CP groups were given alone LC (10 mg ⁄ kg ⁄ every other day), EA (2 mg ⁄ kg ⁄ every other day) and CP (15 mg ⁄ kg ⁄ week) respectively. One of the last two groups received CP + LC, and the other treated with CP + EA. All treatments were maintained for 8 weeks. At the end of the treatment period, morphological abnormalities of sperm, plasma malondialdehyde (MDA) levels and glutathione (GSH) levels, and GSH-peroxidase (GSH-Px), catalase (CAT) and superoxide dismutase (SOD) activities in erythrocytes, and testicular histopathological changes were examined. CP administration caused statistically significant increases in tail and total abnormality of sperm, plasma MDA level and erythrocyte SOD activity, and decreases in erythtocyte CAT activity, diameters of seminiferous tubules, germinal cell layer thickness and Johnsen's Testicular Score along with degeneration, necrosis, immature germ cells, congestion and atrophy in testicular tissue. However, LC or EA treatments to CP-treated rats markedly improved the CP-induced lipid peroxidation, and normalized sperm morphology and testicular histopathology. In conclusion, CP-induced lipid peroxidation leads to the structural damages in spermatozoa and testicular tissue of rats, and also LC or EA have a protective effect on these types of damage.

show abstract

“…23,24 The bone marrow Mn test is a well-known in vivo assay for the assessment of genotoxicity in animals such as mice and rats. All groups treated with CP had the drug injected intraperitoneally, 25 while three type of honey was administered by Oral Intubation (O.I.)…”

Section: Mn Assaymentioning

confidence: 99%

Investigation of role magnetized water used in supplementary feeding for honeybees to modulate the genotoxic side effects induced by cyclophosphamide in mice bone marrow cells

Farhan¹,

Chechan

Alkinani

2017

J. contemp. med. sci.

View full text Add to dashboard Cite

Objective To evaluate the possible protective role of honey formed through innovative way by using magnetized water instead of tab water in supplementary feeding for bees (HW) and compared with two honey types formed by two different feeding solutions; sugar syrup (HS) and nectar of flowers (HF) against cyclophosphamide (CP) genotoxicity in mice bone marrow cells, by using the micronucleus assay (MN). Methods Mice were divided into 8 groups from 3 animals each. G1 as control. G2 were exposed to CP (40 mg/kg), G3, G4, and G5 received two dose of three types of honey at doses (300, 600 mg/kg) respectively. The other groups G6, G7 and G8 were supplemented with three types honey (300 mg/kg) in three different experimental protocols, as pre 2 h, post 2 h, and concomitant treatment for 7 and 14 days. Results Examination and analysis of MN showed no mutagenic effect of three types honey per se doses, especially in low dose (300 mg/kg). Meanwhile, CP induced a significant (P < 0.01) increase in MN frequency. While dual treatment with groups of honey HW caused a significant reduction in MN induced by CP in bone marrow cells in a time-dependant manner. Also, the results confirmed the protective efficacy of HW group and/or HF group as compared with HS group, against CP-toxicity. Conclusion Our study suggests using magnetized water in supplementary feeding of bee, that could give the honey protective effect against genotoxicity induced by CP, it is also fosters antioxidant activity of honey constituents. Therefore, honey HW can be used as an adjuvant with chemotherapeutic agents for minimizing the genotoxic side effects of the anticancer drug CP.

show abstract

Chemoprotective effects of hesperidin against genotoxicity induced by cyclophosphamide in mice bone marrow cells

Cited by 73 publications

References 15 publications

Antigenotoxicity protection of Carapa guianensis oil against mitomycin C and cyclophosphamide in mouse bone marrow

Antigenotoxicity protection of Carapa guianensis oil against mitomycin C and cyclophosphamide in mouse bone marrow

Toxic Effect of Cyclophosphamide on Sperm Morphology, Testicular Histology and Blood Oxidant‐Antioxidant Balance, and Protective Roles of Lycopene and Ellagic Acid

Investigation of role magnetized water used in supplementary feeding for honeybees to modulate the genotoxic side effects induced by cyclophosphamide in mice bone marrow cells

Contact Info

Product

Resources

About