Chemoprotective effect of ascorbic acid on cyclophosphomide induced oral toxicity

Al-Refai, Ali Sultan

doi:10.15218/zjms.2017.002

Cited by 2 publications

(4 citation statements)

References 20 publications

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“…In the present study, H&E examination of cyclophosphamide group (group II) showed areas of atrophied epithelium, areas of keratinocyte degeneration and thinning of the epithelial covering. This result agreed with Al-Refai (13) . In his study, he injected 40 albino-rats with a single dose of IP CTX (300mg/kg) each to induce mucositis and found marked pathological changes like decrease in epithelium and keratin thickness on the ventral surface of the tongue mucosa of rats.…”

Section: Discussionsupporting

confidence: 90%

“…The H&E examination of connective tissue of cyclophosphamide group revealed high inflammatory cell infiltrate and dilated vasculature, this finding is congruent with Saghir et al (15) who observed significant increase in inflammatory cells in lung alveolar tissues after single IP dose of cyclophosphamide. The same finding was also stated in Al-Refai (13) study where severe inflammatory cell infiltrate and dilated blood vessels were observed in the connective tissue of the ventral surface of the tongue in cyclophosphamide injected rats.…”

Section: Discussionsupporting

confidence: 86%

“…These chemicals cause severe inflammatory response that attribute to the increased vascular permeability and tissue damage. The release of free radicals released by cyclophosphamide metabolism also contribute to the cell damage and degeneration observed in group II as observed by Al-Refai (13) .…”

Section: Discussionmentioning

confidence: 77%

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The Effect of Stem Cells Exosomes on Circumvallate Papillae of Albino-Rats Subjected to Cyclophosphamide Chemotherapy

2024

The Egyptian Journal of Hospital Medicine

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Background: There is an increasing attention towards stem cells exosomes and their regenerative potential, bonemarrow derived stem cells exosomes was shown to enhance tissue regeneration. Objective: To explore the effect of bone-marrow derived stem cells exosomes on circumvallate papillae subjected to cyclophosphamide chemotherapy.Methods: Forty-four adult male albino-rats, weighing between 180 and 200grams were used. Two rats were used as a source of bone-marrow stem cells exosomes and the rest were divided equally into 3 groups; 14 rats each as follows:Group I: Control; Group II: Cyclophosphamide group, this group received a single dose (150mg/kg) of cyclophosphamide intraperitoneal; Group III: exosomes group, this group received a single dose (150mg/kg) of cyclophosphamide intraperitoneal then after two days, they received single dose of bone-marrow mesenchymal-stem cells (BM-MSCs)-derived exosomes (100μg) suspended in 1ml phosphate buffered saline intraperitoneal. All groups were sacrificed on day 9 of the experiment. Circumvallate papillae were examined histologically and ultra-structurally.The inflammatory cell counts and percentage of cytoplasmic vacuolation were statistically analyzed.Results: A significant improvement in the shape and size of cells of circumvallate papillae was noticed in both epithelium and connective tissue compared to the samples subjected to cyclophosphamide only, the ultrastructural examination of the tissues showed significant improvement in all cellular organelles especially nuclei shapes, endoplasmic reticulum and mitochondria. A statistically significant decrease in inflammatory cells and vacuolation percentage was also revealed. Conclusion:Bone-marrow derived stem cells exosomes could be considered an effective regenerative treatment for cyclophosphamide induced circumvallate papillae damage.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 86%

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The Effect of Stem Cells Exosomes on Circumvallate Papillae of Albino-Rats Subjected to Cyclophosphamide Chemotherapy

2024

The Egyptian Journal of Hospital Medicine

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“…Some investigators stated that 5-Flurouracil lead to mucosal inflammation that was proved through the elevated expression of many cytokines as tumor necrosis factor-α (TNF-α) and interleukin-1(IL-1), which in turn start the inflammatory response. Furthermore, this inflammation weakens the mucosal membrane barriers which enhance its bacterial penetration, these bacteria will attract the mononuclear cells leading to inflammation and finally mucosal ulceration (33) .…”

Section: Data Expressed As Mean ± Sd Sd: Standard Deviation P: Probab...mentioning

confidence: 99%

Effect of Platelet rich plasma against the cytotoxicity induced by 5-Flurouracil in albino rats’ tongue (Histological and Immunohistochemical study)

Moawad

Abobaker

2022

Egyptian Dental Journal

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Introduction: 5-Flurouracil (5-FU) is considered the backbone of most of chemotherapy regimens, it has been established for the treatment of several neoplasms but one of its most common complications is oral mucositis including tongue epithelium.Aim: This study aimed to explore the effect of Platelet rich plasma (PRP) against the cytotoxicity induced by 5-Flurouracil in albino rats' tongue.Material & Methods: forty male Albino rats were used in this study, 10 rats were used as PRP donors and the other 30 rats were classified into 3 groups, 10 rats each: Group I: 60 mg/kg of saline was injected ip. on day zero, and other 40 mg/kg was injected on the second day. Group II: 60 mg/ kg of 5-FU was injected ip. on day zero, and other 40 mg/kg was injected on the second day. Group III: 60 mg/kg of 5-FU was injected ip. on day zero, and other 40 mg/kg was injected on the second day then after 24 hours, 1ml/rat/day of PRP was injected locally into the tongue mucosa for 3 weeks. The animals were sacrificed after 1 week of the second injection in group I and II and at end of third week of PRP injection in group III. specimens were prepared for histological examination and immunohistochemical staining for CD3.Results: Histological examination revealed significant improvement of mucosal tongue epithelium and connective tissue in group III samples when compared to group II, supported by immunohistochemical results of CD3. Conclusion:PRP has accelerating healing effect on tongue epithelium and connective tissue.

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Chemoprotective effect of ascorbic acid on cyclophosphomide induced oral toxicity

Cited by 2 publications

References 20 publications

The Effect of Stem Cells Exosomes on Circumvallate Papillae of Albino-Rats Subjected to Cyclophosphamide Chemotherapy

The Effect of Stem Cells Exosomes on Circumvallate Papillae of Albino-Rats Subjected to Cyclophosphamide Chemotherapy

Effect of Platelet rich plasma against the cytotoxicity induced by 5-Flurouracil in albino rats’ tongue (Histological and Immunohistochemical study)

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