Chemopreventive properties of the ethanol extract of chinese licorice () root: induction of apoptosis and G1 cell cycle arrest in MCF-7 human breast cancer cells

Jo, Eunhye; Kim, Sung Hoon; Ra, Jeong-Chan; Kim, Sung-Ran; Cho, Sung-Dae; Jung, Ji‐Won; Yang, Se‐Ran; Park, Joon-Suk; Hwang, Jae-Woong; Aruoma, Okezie I.; Kim, Tae-Yung; Lee, Yong-Soon; Kang, Kyung‐Sun

doi:10.1016/j.canlet.2004.12.038

Cited by 98 publications

(72 citation statements)

References 24 publications

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“…Licorice root contains alkaloids, polysaccharides, and flavonoids (32). Previous studies have reported licorice-induced cancer-specific cell death in various cancer cell types, including oral (7,11), prostate (13), colon (33) and breast cancer (34,35), through the inhibition of proliferation (11) and metastasis (18), cell-cycle arrest (13,14), and apoptotic cell death (14). Licochalcone-A, a chalconoid and a type of natural phenol, has been isolated from the root of the licorice plant and has exhibited various pharmacological effects, including antimalarial, anticancer, antibacterial and antiviral properties (7).…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies on the development of chemotherapeutic agents have been focused on inducing cancer-specific cell death through the modulation of apoptosis, or programmed cell death. Furthermore, to overcome the side effects of current chemotherapeutic agents, studies have focused on the anti-cancer activity of natural compounds purified from herbal plants (7,(11)(12)(13)(14)(15). As a result of these studies, the US Food and Drug Administration approved the clinical use of a medicinal herbal plant-derived natural compound with anti-cancer activity for cancer therapy (16).…”

Section: Licochalcone-e Induces Caspase-dependent Death Of Human Pharmentioning

confidence: 99%

“…Furthermore, the anticancer activity of licorice has been reported in breast (18,19), oral (11), colon (12) and prostate cancer (13). Licorice inhibits metastasis (18), has anti-proliferative properties (19), increases apoptosis and may cause cell cycle arrest (14). Recently, the authors demonstrated that licochalcone-A, a natural phenolic chalconoid purified from licorice (15), induces caspase-dependent apoptotic FaDu cell death through the extrinsic and intrinsic apoptotic signaling pathways.…”

Section: Licochalcone-e Induces Caspase-dependent Death Of Human Pharmentioning

confidence: 99%

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Licochalcone-E induces caspase-dependent death of human pharyngeal squamous carcinoma cells through the extrinsic and intrinsic apoptotic signaling pathways

Cho

Kang

et al. 2017

Oncology Letters

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Abstract. The aim of the present study was to investigate licochalcone-E (Lico-E)-induced apoptosis and the associated apoptotic signaling pathway in FaDu cells, a human pharyngeal squamous carcinoma cell line. Treatment with Lico-E exhibited significant cytotoxicity on FaDu cells in a concentration-dependent manner. The IC 50 value of Lico-E in FaDu cells was ~50 µM. Treatment with Lico-E increased the number of dead FaDu cells. Furthermore, chromatin condensation, which is associated with apoptotic cell death, was observed in FaDu cells treated with Lico-E for 24 h. By contrast, Lico-E did not produce cytotoxicity or increase the number of dead cells when applied to human normal oral keratinocytes (hNOKs). Furthermore, chromatin condensation was not observed in hNOKs treated with Lico-E. Treatment with Lico-E increased the expression of Fas ligand and the cleaved form of caspase-8 in FaDu cells. Furthermore, treatment with Lico-E increased the expression of pro-apoptotic factors, including apoptosis regulator BAX, Bcl-2-associated agonist of cell death, apoptotic protease-activating factor 1, caspase-9 and tumor suppressor p53, while decreasing the expression of anti-apoptotic factors, including apoptosis regulator Bcl-2 and Bcl-2-like protein 1 in FaDu cells. The expression of cleaved caspases-3 and poly (ADP-ribose) polymerase was significantly upregulated following treatment with Lico-E in FaDu cells, while Lico-E-induced apoptotic FaDu cell death was partially suppressed by treatment with Z-VAD-FMK, a pan caspase inhibitor. Therefore, Lico-E-induced oral cancer (OC) cell-specific apoptosis is mediated by the death receptor-dependent extrinsic and mitochondrial-dependent intrinsic apoptotic signaling pathways. In conclusion, these data suggested that Lico-E exhibits potential chemopreventive effects and warrants further developed as a chemotherapeutic agent against OC.

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Section: Discussionmentioning

confidence: 99%

Section: Licochalcone-e Induces Caspase-dependent Death Of Human Pharmentioning

confidence: 99%

Section: Licochalcone-e Induces Caspase-dependent Death Of Human Pharmentioning

confidence: 99%

See 1 more Smart Citation

Licochalcone-E induces caspase-dependent death of human pharyngeal squamous carcinoma cells through the extrinsic and intrinsic apoptotic signaling pathways

Cho

Kang

et al. 2017

Oncology Letters

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“…Recent studies have reported that licorice roots contain multiple bioactive components such as saponins, flavonoids, and coumarins [13][14][15]. Furthermore, licorice has been shown to have anticancer effects in various types of cancer such as colon [16], lung [17,18], breast [19,20], prostate [21], and oral cancers [11] through metastatic inhibition [19], anti-proliferative properties [20], increased apoptosis [11,22], and cell cycle arrest [23].…”

Section: Discussionmentioning

confidence: 99%

Anti-tumor effect of licochalcone-E is mediated by caspase-dependent apoptosis through extrinsic and intrinsic apoptotic signaling pathways in KB cancer cells

et al. 2017

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·············································································································································································This study investigates the anti-cancer effects of licochalcone-E (Lico-E), a phenolic chalconoid derived from the genus Glycyrrhiza, in the human KB squamous cancer cell. Concentration-dependent cytotoxicity in KB cells increased following 24 hours of treatment with 12.5, 25, or 50 µg/ml Lico-E, with an estimated IC 5 0value of approximately 25 µg/ml. Chromatin condensation, a typical apoptotic phenomenon, was observed in KB cells treated with Lico-E. Consistent with this finding, Lico-E increased caspase-3 activity in KB cells. FasL, a death ligand associated with extrinsic apoptotic signaling pathways, was significantly up-regulated by Lico-E treatment. Subsequently, the pro-apoptotic factor caspase-8, a part of the extrinsic apoptotic signaling pathway, was activated in a concentrationdependent manner by Lico-E treatments. Expression of anti-apoptotic factors such as Bcl-2 and Bcl-xL, components of the mitochondriadependent apoptotic signaling pathway, significantly decreased following Lico-E treatment. Conversely, expression of pro-apoptotic factors such as Bax, Bad, Apaf-1, and caspase-9 increased with Lico-E concentrations. Finally, Lico-E activated caspase-3 and Poly (ADP-ribose) polymerase (PARP) to induce cell death. Z-VAD-fmk significantly inhibited cell death through suppression of caspase-3 expression in KB cells treated with Lico-E. Taken together, Lico-E induces KB cell death through death receptor and mitochondriadependent apoptotic signaling pathways.

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“…. 80% 에탄올 추출물은 estradiol에 근접 (>95%)한 에스트로겐 활성을 나타내 는데 27) , 80% 에탄올 추출물 100 ㎍/mL은 MCF-7에서 증식보다는 종양 억제 유전자인 p53과 세포자멸사 전 구단백질인 Bax를 상향조절하여 오히려 세포자멸사를 야기했다 28) . 또한 자궁내막선암세포 Ishikawa에 처리 한 결과, 약한 ER 결합, progesterone 수용체 및 에스 트로겐 유발 유전자인 pS2 (presenelin-2) mRNA 발현 을 보였다 29) .…”

Section: 감초 (Glycyrrhizae Radix)unclassified

The Review of the Herbal Medicines with Phytoestrogenic Effect

Lee¹,

Kim²,

Lee³

et al. 2015

The Journal of Korean Oriental Pediatrics

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Chemopreventive properties of the ethanol extract of chinese licorice () root: induction of apoptosis and G1 cell cycle arrest in MCF-7 human breast cancer cells

Cited by 98 publications

References 24 publications

Licochalcone-E induces caspase-dependent death of human pharyngeal squamous carcinoma cells through the extrinsic and intrinsic apoptotic signaling pathways

Licochalcone-E induces caspase-dependent death of human pharyngeal squamous carcinoma cells through the extrinsic and intrinsic apoptotic signaling pathways

Anti-tumor effect of licochalcone-E is mediated by caspase-dependent apoptosis through extrinsic and intrinsic apoptotic signaling pathways in KB cancer cells

The Review of the Herbal Medicines with Phytoestrogenic Effect

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