Chemopreventive Properties and Toxicity of Kelulut Honey in<i>Sprague Dawley</i>Rats Induced with Azoxymethane

Yazan, Latifah Saiful; Zali, Muhamad Firdaus Shyfiq Muhamad; Ali, Razana Mohd; Zainal, Nurul Amira; Esa, Nurulaidah; Sapuan, S. M.; Ong, Yong Sze; Tor, Yin Sim; Gopalsamy, Banulata; Voon, Fui Ling; Alwi, Sharifah Sakinah Syed

doi:10.1155/2016/4036926

Cited by 44 publications

(22 citation statements)

References 17 publications

(16 reference statements)

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“…To observe the effect of SBH on ACF, SBH was administered orally (1183 mg/kg body weight) and had proven to reduce the total number of ACF and aberrant crypt and crypt multiplicity. Therefore, SBH is neither harmful nor toxic to the animal [76]. An in vitro study was also conducted to screen for the cytotoxic activity of different stingless bee products against five human cancer cell line, namely, BT474 (ductal carcinoma and lung undifferentiated cancer), HepG29 (liver hepatoblastoma), KatoIII (gastric carcinoma), and SW620 (adenocarcinoma), whereby the crude extracts of SBH showed great cytotoxicity effects towards HepG2 cell line, while propolis crude extracts exhibit high cytotoxicity effects towards all the human cancer cell line [77].…”

Section: Therapeutic Effects Of Stingless Bee Honey and European Bmentioning

confidence: 99%

Therapeutic Properties of Stingless Bee Honey in Comparison with European Bee Honey

Amin

Sabri

Mohammad

et al. 2018

Advances in Pharmacological Sciences

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Both honeybees (Apis spp.) and stingless bees (Trigona spp.) produce honeys with high nutritional and therapeutics value. Until recently, the information regarding potential health benefits of stingless bee honey (SBH) in medical databases is still scarce as compared to the common European bee honey (EBH) which is well known for their properties as therapeutic agents. Although there have been very few reports on SBH, empirically these products would have similar therapeutic quality as the EBH. In addition, due to the structure of the nest, few studies reported that the antimicrobial activity of SBH is a little bit stronger than EBH. Therefore, the composition of both the types of honey as well as the traditional uses and clinical applications were compared. The results of various studies on EBH and SBH from tissue culture research to randomised control clinical trials were collated in this review. Interestingly, there are many therapeutic properties that are unique to SBH. Therefore, SBH has a great potential to be developed for modern medicinal uses.

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Section: Therapeutic Effects Of Stingless Bee Honey and European Bmentioning

confidence: 99%

Therapeutic Properties of Stingless Bee Honey in Comparison with European Bee Honey

Amin

Sabri

Mohammad

et al. 2018

Advances in Pharmacological Sciences

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“…In contrast to other local honey species, KH has been reported to possess stronger antioxidant activity and higher phenolic content [19,20]. It also has the potential to be used in the treatment of various medical conditions due to its anti-bacterial, anti-fungal and anti-cancer properties [21][22][23]. However, the effects of KH on MetS are still unknown.…”

Section: Introductionmentioning

confidence: 99%

The Beneficial Effects of Stingless Bee Honey from Heterotrigona itama against Metabolic Changes in Rats Fed with High-Carbohydrate and High-Fat Diet

Ramli

Chin

Zarkasi

et al. 2019

IJERPH

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Metabolic syndrome (MetS) is a group of conditions including central obesity, hyperglycemia, dyslipidemia, and hypertension that increases the risk for cardiometabolic diseases. Kelulut honey (KH) produced by stingless honey bees has stronger antioxidant properties compared to other honey types and may be a functional food against MetS. This study aimed to determine the efficacy of KH in preventing metabolic changes in rats with MetS induced by high-carbohydrate and high-fat (HCHF) diet. Male Wistar rats were randomly assigned to the control (C), HCHF diet-induced MetS (S), and MetS supplemented with KH (K) groups. The K group was given KH (1 g/kg/day) for eight weeks. Compared to the control, the S group had significant higher omental fat mass, serum triglyceride, systolic blood pressure, diastolic blood pressures, adipocyte area, and adipocyte perimeter (p < 0.05). KH supplementation significantly prevented these MetS-induced changes at week 16 (p < 0.05). Several compounds, including 4-hydroxyphenyl acetic acid, coumaric and caffeic acids, had been detected via liquid chromatography-mass spectrometry analysis that might contribute to the reversal of these changes. The beneficial effects of KH against MetS-induced rats provide the basis for future KH research to investigate its potential use in humans and its molecular mechanisms in alleviating the disease.

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“…A number of natural products have anti-CRC effects, including honey, bee pollen, propolis and Brassicaceae extracts (5,6). The present study focused on the effect of sulforaphane, a phytochemical extracted from Brassicaceae.…”

Section: Introductionmentioning

confidence: 99%

Sulforaphane‑induced epigenetic regulation of Nrf2 expression by DNA methyltransferase in human Caco‑2 cells

et al. 2019

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The present study aimed to investigate the mechanism underlying sulforaphane-mediated epigenetic regulation of nuclear factor-erythroid derived 2-like 2 (Nrf2) expression in human colon cancer. Proteins were extracted from normal Caco-2 cells using sulforaphane and 5-aza-2'-deoxycytidine (5-Aza) combined with trichostatin A (TSA). The mRNA and protein expression levels and activity of DNA methyltransferase 1 (DNMT1) were determined. Methylation-specific polymerase chain reaction and bisulfite genomic sequencing were also used to measure the methylation levels of CpG sites in the Nrf2 promoter region. Nrf2 expression was measured using reverse transcription-quantitative PCR and western blot analysis. The results demonstrated that sulforaphane did not affect DNMT1 mRNA expression levels. DNMT1 protein expression was inhibited by sulforaphane and 5-Aza co-treatment with TSA. Nrf2 promoter methylation decreased significantly in the sulforaphane group compared with the control group. Nrf2 promoter methylation level in the 5-Aza+TSA group was the lowest among all groups. Nrf2 mRNA levels exhibited significant differences between the sulforaphane-treated and control groups, as well as between the 5-Aza+TSA and control groups, and the sulforaphane-treated and 5-Aza+TSA groups. Nrf2 protein expression was also inhibited by sulforaphane, as well as 5-Aza co-treatment with TSA. The results revealed that sulforaphane may promote demethylation of the Nrf2 promoter region to increase activation of Nrf2, which induces chemoprevention of colon cancer.

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Chemopreventive Properties and Toxicity of Kelulut Honey inSprague DawleyRats Induced with Azoxymethane

Cited by 44 publications

References 17 publications

Therapeutic Properties of Stingless Bee Honey in Comparison with European Bee Honey

Therapeutic Properties of Stingless Bee Honey in Comparison with European Bee Honey

The Beneficial Effects of Stingless Bee Honey from Heterotrigona itama against Metabolic Changes in Rats Fed with High-Carbohydrate and High-Fat Diet

Sulforaphane‑induced epigenetic regulation of Nrf2 expression by DNA methyltransferase in human Caco‑2 cells

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