Chemopreventive potential of esculetin in 7,12-dimethylbenz(a)anthracene-induced hamster buccal pouch carcinogenesis

Selvasundaram, R.; Manoharan, Shanmugam; Buddhan, Rajamanickam; Neelakandan, Mani; Naidu, Radhakrishnan Murali

doi:10.1007/s11010-018-3321-0

Cited by 16 publications

(14 citation statements)

References 35 publications

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“…In the current study, hamsters in the second group (B) that were subjected to topical application of DMBA alone, all showed well visible tumors in their buccal pouches. The results are in agreement with Manoharan et al, (2) and Selvasundaram et al, (26) mentioned that a 100% tumor incidence was found in all the hamsters receiving DMBA carcinogen application included in their studies, thus suggesting DMBA to be considered a powerful tumor inducer. Contrastingly, in the study conducted by Li et al, (19) Only 77% of the hamsters developed buccal pouch tumors, and that could be attributed to the different animal species.…”

Section: Discussionsupporting

confidence: 92%

Chemopreventive Effect of Green Tea and Curcumin in Induced Oral Squamous Cell Carcinoma: An Experimental Study

Saleh

Darwish

Nouaem

et al. 2020

Alexandria Dental Journal

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INTRODUCTION: Oral squamous cell carcinoma (OSCC) has the highest mortality rates among all carcinomas and is the most common head and neck cancer. Several natural compounds and micronutrients have been under investigation for their efficacy in head and neck cancer chemoprevention. Green tea contains various cancer preventive catechins that have a role in preventing cancer recurrence in various organs in humans. Curcumin, a natural polyphenol, is one of the most investigated biomolecules from Mother Nature. Curcumin has been shown to exert significant growth inhibitory effect on oral precancerous and carcinoma cell lines, and the effect is synergistic with epigallocatechin gallate, the most abundant polyphenol in tea. OBJECTIVES: To assess the chemopreventive effects of green tea and curcumin through induction of hamster buccal pouch carcinoma by using an apoptotic marker and compare their effect each alone and in combination. MATERIALS AND METHODS: Squamous cell carcinoma was chemically induced in fifty Syrian golden hamsters divided into 5 groups (10 each). The first group was used as normal control group. The second group received the carcinogenic agent only. The other three groups received green tea, curcumin and combination of both, respectively. RESULTS: Normal control group (A) revealed neither pathological nor inflammatory changes in the buccal pouch with 1.72% of the cells underwent apoptosis while the cells of positive control group (B) resulted in 11.57% apoptosis. In the study groups, treatment of the cells with green tea (C), curcumin (D) and both of them (E) resulted in 82.22 %, 78.91%, 96.63% apoptosis respectively. The fluorescent image by confocal laser scanning in group B showed increase of the red fluorescence in the nucleus and cytoplasm of the squamous cell carcinoma cells indicating high proliferating cells while the fluorescent image of group C, D and E showed decrease of the red florescence in the nuclei of the squamous cell carcinoma cells indicating low proliferation. CONCLUSIONS: Green tea and curcumin have a significant chemopreventive effect against oral carcinogenesis and the combination of both agents has a better effect.

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Section: Discussionsupporting

confidence: 92%

Chemopreventive Effect of Green Tea and Curcumin in Induced Oral Squamous Cell Carcinoma: An Experimental Study

Saleh

Darwish

Nouaem

et al. 2020

Alexandria Dental Journal

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“…The first immunocompetent murine model was the hamster cheek-pouch (69), primarily used for chemopreventive studies (70–73). The buccal pouch mucosa is exposed to 9,10 dimethyl-1,2 benzathracene (DMBA), a carcinogen.…”

Section: Immunocompetent – Chemically-induced Modelsmentioning

confidence: 99%

Immune-relevant aspects of murine models of head and neck cancer

Rossa

D’Silva

2019

Oncogene

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Head and neck cancers (HNC) cause significant mortality and morbidity. There have been few advances in therapeutic management of HNC in the past 4 to 5 decades, which supports the need for studies focusing on HNC biology. In recent years, increased recognition of the relevance of the host response in cancer progression has led to novel therapeutic strategies and putative biomarkers of tumor aggressiveness. However, tumor-immune interactions are highly complex and vary with cancer type. Pre-clinical, in vivo models represent an important and necessary step in understanding biological processes involved in development, progression and treatment of HNC. Rodents (mice, rats, hamsters) are the most frequently used animal models in HNC research. The relevance and utility of information generated by studies in murine models is unquestionable, but it is also limited in application to tumor-immune interactions. In this review, we present information regarding the immune-specific characteristics of the murine models most commonly used in HNC research, including immunocompromised and immunocompetent animals. The particular characteristics of xenograft, chemically-induced, syngeneic, transgenic, and humanized models are discussed in order to provide context and insight for researchers interested in the in vivo study of tumor-immune interactions in HNC.

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“…Based on the history of drug development, natural product-derived compounds have been demonstrated to have unique capabilities for inducing apoptosis more commonly in cancer cells than in normal cells [5][6][7]. This is likely because their unique structures are strictly associated with their binding ability to specific molecules involved in particular biological and/or metabolic pathways [8].…”

Section: Introductionmentioning

confidence: 99%

Fli-1 Activation through Targeted Promoter Activity Regulation Using a Novel 3’, 5’-diprenylated Chalcone Inhibits Growth and Metastasis of Prostate Cancer Cells

et al. 2020

IJMS

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The friend leukemia integration 1 (Fli-1) gene is involved in the expression control of key genes in multiple pathogenic/physiological processes, including cell growth, differentiation, and apoptosis; this implies that Fli-1 is a strong candidate for drug development. In our previous study, a 3′,5′-diprenylated chalcone, (E)-1-(2-hydroxy-4-methoxy-3,5-diprenyl) phenyl-3-(3-pyridinyl)-propene-1-one (C10), was identified as a novel anti-prostate cancer (PCa) agent. Here, we investigated the molecular mechanisms underlying the anti-cancer effects of C10 on the growth, metastasis, and invasion of PC3 cells in vitro. Our results show that C10 exhibited a strong inhibitory effect on proliferation and metastasis of PC3 cells via several cellular and flow cytometric analyses. Further mechanism studies revealed that C10 likely serves as an Fli-1 agonist for regulating the expression of Fli-1 target genes including phosphatidylinositol 3-kinase (P110), murine double minute2 (MDM2), B-cell lymphoma-2 (Bcl-2), Src homology-2 domain-containing inositol 5-phosphatase 1 (SHIP-1), and globin transcription factor-1 (Gata-1) as well as the phosphorylation of extracellular-regulated protein kinases 1 (ERK1). Further, we confirmed that C10 can regulate the expressions of vascular endothelial growth factor 1 (VEGF-1), transforming growth factor-β2 (TGF-β2), intercellular cell adhesion molecule-1 (ICAM-1), p53, and matrix metalloproteinase 1 (MMP-1) genes associated with tumor apoptosis, migration, and invasion. Thus, C10 exhibits stronger anticancer activity with novel molecular targets and regulatory molecular mechanisms, indicating its great potency for development as a novel targeted anticancer drug.

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Chemopreventive potential of esculetin in 7,12-dimethylbenz(a)anthracene-induced hamster buccal pouch carcinogenesis

Cited by 16 publications

References 35 publications

Chemopreventive Effect of Green Tea and Curcumin in Induced Oral Squamous Cell Carcinoma: An Experimental Study

Chemopreventive Effect of Green Tea and Curcumin in Induced Oral Squamous Cell Carcinoma: An Experimental Study

Immune-relevant aspects of murine models of head and neck cancer

Fli-1 Activation through Targeted Promoter Activity Regulation Using a Novel 3’, 5’-diprenylated Chalcone Inhibits Growth and Metastasis of Prostate Cancer Cells

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