Chemopreventive Effects of the Juice ofVitis coignetiaePulliat on Two-Stage Mouse Skin Carcinogenesis

Abstract

Our study revealed the inhibitory effect of Vitis coignetiae Pulliat, known as Yamabudo in Japan, at the stages of multi-step carcinogenesis. The juice of Vitis coignetiae (Y-grape juice) was antimutagenic toward dimethylbenzo[a]anthracene (DMBA), aflatoxin B1, and benzo[a]pyrene in the Ames test. The Y-grape juice was also antigenotoxic in the micronucleus test using HepG2 cells toward DMBA and aflatoxin B1. Topical and oral administration of the Y-grape juice to mice inhibited the induction of inflammation of 12-O-tetradecanoylphorbol-13-acetate (TPA). Topical and oral administration of the Y-grape juice significantly decreased the incidence and mean number of tumors in mice skin with the 2-stage tumorigenesis protocol. To elucidate the mechanisms underlying the antiinflammatory and antitumor promotion activity of the Y-grape juice, the effect of Y-grape juice on cyclooxygenase-2 (COX-2) activity in mouse ear treated with TPA was studied. Both topical and oral application of the Y-grape juice inhibited the TPA-induced increase in COX-2 activity. Caftaric acid, isolated and identified from the Y-grape juice, was antimutagenic toward DMBA and prevented TPA-induced inflammation in mice, suggesting caftaric acid participates in chemopreventive effect/activities of Y-grape juice.

Bioactive compounds of juices from two Brazilian grape cultivars

“…Calibration curves were prepared for these 21 standard phenolic compounds using the same chromatographic conditions. Table shows the concentration range of each standard used for the calibration curves, standards purity (SP), equations of the calibration curves, coefficients of determination ( R ), detection limits (LD), quantification limits (LQ) and assay repeatability through coefficient of variation (CV) . Data collection and analysis were carried out using Empore TM 2 software (Milford, CT, USA).…”

“…Grapes, grape juice and wine have shown both in vitro and in vivo potential to prevent several chronic diseases. The benefits stem from their high polyphenol content, which are secondary metabolites produced by plants as a defense mechanism against environmental stress . The protective effects against health problems such as cancer and cardiovascular diseases may be attributed to their antioxidant activity and antiproliferative properties .…”

“…The protective effects against health problems such as cancer and cardiovascular diseases may be attributed to their antioxidant activity and antiproliferative properties . Furthermore, interest in polyphenols has increased owing to their potential activity as antitoxic and cytoprotective molecules with a modulatory action in inflammatory processes and neurodegenerative diseases …”

Chlorogenic and Caftaric Acids in Liver Toxicity and Oxidative Stress Induced by Methamphetamine

“…Group 1 which was the control group was injected (i.p) with saline (1 mL/kg) twice a day over seven-day period. Groups 2, 3, and 4 were injected (i.p) with METH (10 mg/kg) twice a day over seven-day period, where group 3 was injected once (i.p) with 60 mg/kg chlorogenic acid [35] and group 4 was injected once (i.p) with 40 mg/kg caftaric acid [36], respectively, one day before METH injections.…”

Inhibitory effect of Actinidia arguta on mutagenesis, inflammation and two-stage mouse skin tumorigenesis

“…In our recent study, the juice of Vitis coignetiae , which is rich in polyphenols, significantly inhibited the mutagenicity and clastogenicity of several mutagens using the Ames test and micronucleus test [7]. Topical application of the juice of V. coignetiae significantly decreased the incidence and multiplicity of tumors in mice skin using a two-stage tumorigenesis protocol [8]. Actinidia arguta , known as sarunashi in Japan and hardy kiwi in English name, is a vine tree native to east-Asia, including Japan [9], that produces small fruit consumed in the form of fresh fruit and jam.…”