Chemopreventive effects of mate against mouse mammary and colon carcinogenesis

Abstract

The present study evaluated the chemopreventive potential of mate tea-like intake on mammary and colon carcinogenesis initiated by 7,12-dimethylbenz(a)antracene (DMBA) and 1,2-dimethylhydrazine (DMH) in female Swiss mice. After the initiation period, the animals received basal diet and organic mate tea-like, conventional mate tea-like, or green tea (positive control) at 2.0% as the drinking fluid during 15 weeks. At week 20, colon and mammary gland were analyzed for preneoplastic and neoplastic lesions development. Colon and mammary gland complexes were processed for cell proliferation analysis, estimated by proliferating cell nuclear antigen labeling index (PCNA-LI%). Specially, organic mate tea-like reduced the values of PCNA-LI% in colonic crypts (p < .003) and in mammary glands (p < .05) in DMBA/DMH-initiated groups. A lower incidence of aberrant crypt foci (ACF) in colon (p = .03) and of hyperplastic and neoplastic lesions in mammary gland (p < .05 and p < .02, respectively) was observed in DMBA/DMH-initiated groups treated with organic mate tea-like. These results suggest that post-initiation treatment with organic mate tea-like inhibited the development of colon and mammary carcinogenesis in a two-step medium-term mouse carcinogenesis model.

Protective effects of lemongrass (Cymbopogon citratus STAPF) essential oil on DNA damage and carcinogenesis in female Balb/C mice

“…once a week from weeks 1 to 5), 1,2‐dimethylhydrazine dihydrochloride [DMH, Tokyo Kasei Industries Co., Japan; four subcutaneous injections (s.c.) of 30 mg kg −1 b.w., twice a week from weeks 3 to 4] and N‐ butyl‐ N‐ (4‐hydroxybutyl)nitrosamine (BBN, Tokyo Kasei Industries Co., Japan; eight doses of 300 mg kg −1 b.w. once a week from weeks 5 to 12; Zapaterini et al ., 2010; de Moura et al ., 2010) plus the gavages of LGEO vehicle (2% Tween 20, five times per week from weeks 14 to 20) LGEO‐treated groups, test groups that received the carcinogens regimen cited above and by gavage LGEO at 125 mg kg −1 or 500 mg kg −1 b.w., respectively (five times per week, from weeks 14 to 20).…”

Yerba mate tea and mate saponins prevented azoxymethane‐induced inflammation of rat colon through suppression of NF‐κB p65ser311 signaling via IκB‐α and GSK‐3β reduced phosphorylation

“…YMT administered during the initiation phase of carcinogenesis induced by diethylnitrosamine inhibited DNA damage and the development of preneoplastic and neoplastic lesions in the esophagus and liver of male Wistar rats . YMT reduced colon carcinogenesis in postinitiation of 1,2‐dimethylhydrazine‐induced aberrant crypt foci (ACF) in female Swiss mice . However, the active compounds were not identified and a mechanism of action has not been proposed.…”

Modifying Effects of Lemongrass Essential Oil on Specific Tissue Response to the Carcinogen N-Methyl-N-Nitrosurea in Female BALB/c Mice

“…Mammary lesions were classified as alveolar/ductal hyperplasia, colon lesions as ACF, and urothelial lesions as simple or nodular hyperplasia, according to previously published criteria. 26,[28][29][30] Under hematoxylin and eosin staining, apoptotic cells were identified by typical morphological criteria as previously described. 26,30,31 Immunohistochemical staining for proliferation cellular nuclear antigen (PCNA) was performed using the streptavidin-biotin peroxidase method, according to previously established procedures.…”

“…26,[28][29][30] Under hematoxylin and eosin staining, apoptotic cells were identified by typical morphological criteria as previously described. 26,30,31 Immunohistochemical staining for proliferation cellular nuclear antigen (PCNA) was performed using the streptavidin-biotin peroxidase method, according to previously established procedures. 32 In brief, deparaffinized 5-lmthick sections were subjected to antigenic detection by 0.1 M citric acid (two cycles of 5 minutes each in a microwave oven) and were treated with 3% hydrogen peroxide for 10 minutes, nonfat milk for 60 minutes, anti-PCNA antibody (1:100 dilution, Dako, Glostrup, Denmark) conjugated with anti-mouse antibody (1:200 dilution, Dako) overnight, and streptavidin-biotin peroxidase solution (1:50 dilution, Vector Laboratories, Burlingame, CA, USA) for 45 minutes.…”

“…26,30 Cell proliferation and apoptosis indexes were analyzed in order to evaluate the LGEO protective effects on acute proliferative and apoptotic responses 162 BIDINOTTO ET AL.…”