Chemopreventive effects of combination of honokiol and magnolol with α-santalol on skin cancer developments

Abstract

α-Santalol is active component of sandalwood oil and has been shown to have chemopreventive effects against chemically and UVBinduced skin cancer development in mice. α-Santalol is also shown to have skin permeation enhancing effects. Honokiol and magnolol isolated from Magnolia officinalis bark extract have also been shown to have chemopreventive effects against chemically and UVB-induced skin cancer in mice. This study was conducted to investigate the combination effects of α-santalol, honokiol and magnolol to study any additive/synergistic effects to lower the doses required for chemoprevention. Pretreatment of combinations of α-santalol with honokiol and magnolol significantly decreased tumor multiplicity upto 75% than control, α-santalol, honokiol and magnolol alone in SKH-1 mice. Combination of α-santalol with honokiol and magnolol also decreased cell viability, proliferation, and enhanced apotosis in comparision to α-santalol, honokiol and magnolol alone in Human epidrmoid carcinoma A431 cells. Overall, the results of present study indicated combinations of α-santalol with honokiol and magnolol could provide chemoprevention of skin cancer at lower doses than given alone.

Insights on the Multifunctional Activities of Magnolol

“…The anticancer activity of magnolol has been intensively studied in colon and liver cancer cells [67], lung squamous carcinoma CH27 cells [68], L5178Y-ML25 lymphoma [69], and B16-BL6 melanoma [69]. Pretreatment of combination of magnolol with honokiol significantly decreases cell viability and proliferation and increases cell apoptosis in human epidermoid carcinoma A431 cells [10]. In U87MG and LN229 human glioma cells, magnolol, coadministrated with honokiol, has been demonstrated to synergistically inhibit the expression of cyclin A, cyclin D1, and cyclin-dependent kinase (CDK) 2, CDK4, and CDK6, resulting in cell cycle arrest.…”

“…Both magnolol and honokiol are isolated from the stem bark of a traditional Chinese herbal medicine Magnolia officinalis , which has been used for management of nervous disturbance, abdominal distention or disorders, gastrointestinal food stagnancy, and coughing and dyspnea [1]. Magnolol has showed a wide spectrum of beneficial activities, including anti-inflammation [2, 3], antimicroorganism [4, 5], antioxidation [6, 7], antiangiogenesis [8, 9], anticancer [10–12], neuroprotection [13, 14], cardiovascular protection [15, 16], and lipolysis activities [17, 18]. However, there are still some differences between magnolol and honokiol in safety and toxicology, which have been reviewed by Sarrica et al (2018)[19].…”