Chemopreventive effect of oleuropein in colitis‐associated colorectal cancer in c57bl/6 mice

Giner, Elisa; Recio, M. C.; Rı́os, José Luis; Cerdá-Nicolás, José Miguel; Giner, Rosa M.

doi:10.1002/mnfr.201500605

Cited by 102 publications

(101 citation statements)

References 38 publications

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“…Nowadays, there is a strong evidence for the protective role of oleuropein against many types of cancer [22,23,24]. More specifically, Acquaviva et al, have recently demonstrated the antiproliferative effect of oleuropein in the androgen sensitive LNCaP and in the androgen insensitive DU145 prostate cancer cell line which are characterized by low and moderate tumorigenicity, respectively [25].…”

Section: Discussionmentioning

confidence: 99%

Oleuropein is a Powerful Sensitizer of Doxorubicin-mediated Killing of Prostate Cancer Cells and Exerts Its Action via Induction of Autophagy

Papachristodoulou¹,

Tsoukala²,

Benaki³

et al. 2018

JCRT

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The phenolic component Oleuropein (OLEU), a bioactive natural product, has recently shown antiproliferative properties. Doxorubicin (DXR) is an anthracycline present in many chemotherapeutic schemes, although limited due to its cardio-toxic effects. Important research effort has been devoted therefore, to reducing DXR toxicity without compromising its antitumor efficacy. The anticancer actions of DXR and OLEU were assessed, on PC-3 prostate cancer cells, while cell cycle distribution and rate of apoptosis were assessed by flow cytometry. The autophagic process was determined via immunoblotting and immunofluorescent staining. Finally, cell extracts were analyzed by NMR spectroscopy. The present study showed that both DXR and OLEU inhibited PC-3 cells proliferation, while the co-treatment with DXR and OLEU resulted in an additive inhibition. Although the addition of OLEU to DXR did not alter significantly the cell cycle distribution, exhibited by each treatment alone, and produced a marginal increase on the rate of apoptosis, both compounds produced a remarkable induction of autophagy. In addition, treated cells exhibited significant metabolite alterations. This study demonstrates that OLEU, a basic component of the everyday diet, is capable of lowering significantly the cytotoxic dose of DXR, while obtaining an important anti-proliferative effect in prostate cancer cells.

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Section: Discussionmentioning

confidence: 99%

Oleuropein is a Powerful Sensitizer of Doxorubicin-mediated Killing of Prostate Cancer Cells and Exerts Its Action via Induction of Autophagy

Papachristodoulou¹,

Tsoukala²,

Benaki³

et al. 2018

JCRT

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“…Further, it also significantly down regulated the CRC‐related pathways as Wnt/β‐catenin, signal transducer and activators of transcription (STAT)‐3, nuclear factor‐κB (NF‐κB), and phosphatidylinositol‐3‐kinase (P3IK)/Akt. Oleuropein suppressed the Th17 response by lowering Rorγt, T‐cell subsets, IL‐17, CD4, and IFN‐γ as well as IL‐17A and IFN‐γ expression in dextran sulfate sodium acute model (Giner, Recio, Ríos, Cerdá‐Nicolás, & Giner, ). The supplementation of oleuropein (125 mg/kg) in rats prevented the azoxymethane (AOM)‐induced preneoplastic lesions in different colon segments, reducing the severity of crypt dysplasia and DNA damage in peripheral leukocytes.…”

Section: Introductionmentioning

confidence: 99%

Antitumor Perspectives of Oleuropein and Its Metabolite Hydroxytyrosol: Recent Updates

Imran

Nadeem

Gilani

et al. 2018

Journal of Food Science

112

102

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Olive fruit is a significant and promising source of potential bioactive compounds such as oleuropein and hydroxytyrosol. Oleuropein is the ester of elenolic acid and 3,4-dihydroxyphenyl ethanol (HT). It is the main glycoside in olives, the degradation of which results in the formation of hydroxytyrosol in olive oil. Both plays a significant role in the reduction of coronary heart diseases and a certain type of cancers. Both olive oil phenols have an effective role counter to cell proliferation, cell growth, migration, invasion, and angiogenesis. They down regulate the expression of BCL-2 and COX-2 proteins, and reduced DNA damage. Hydroxytyrosol and oleuropein inhibited the multiple stages in colon carcinogenesis; initiation, promotion, and metastasis. They also provide protection against various human cancers including colorectal, skin, breast, thyroid, digestive, lung, brain, blood, and cervical. This review article discusses the anticancer perspectives and mechanisms of oleuropein and hydroxytyrosol in cell cultures and animal and human studies.

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“…In the same animal model, OLE was found to downregulate the Th17 response, a key proinflammatory pathway activated in IBD [16]. These findings led us to hypothesize that OLE may have the potential to reverse chronic inflammation in UC.…”

Section: Introductionmentioning

confidence: 99%

Oleuropein Decreases Cyclooxygenase-2 and Interleukin-17 Expression and Attenuates Inflammatory Damage in Colonic Samples from Ulcerative Colitis Patients

et al. 2017

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Oleuropein (OLE) is the major phenolic secoiridoid of olive tree leaves, and its antioxidant and anti-inflammatory activities have been demonstrated in in vitro and in vivo animal models. The aim of this study was to investigate the activity of OLE in the colonic mucosa from patients with ulcerative colitis (UC). Biopsies obtained during colonoscopy from 14 patients with active UC were immediately placed in an organ culture chamber and challenged with lipopolysaccharide from Escherichia coli (EC-LPS) at 1 μg/mL in the presence or absence of 3 mM OLE. The expression of cyclooxygenase (COX)-2 and interleukin (IL)-17 was assessed in total protein extracts from treated colonic biopsies by Western blotting. Levels of IL-17 were also measured in culture supernatant by ELISA. A microscopic evaluation of the cultured biopsies was performed by conventional histology and immunohistochemistry. The expression of COX-2 and IL-17 were significantly lower in samples treated with OLE + EC-LPS compared with those treated with EC-LPS alone (0.80 ± 0.15 arbitrary units (a.u.) vs. 1.06 ± 0.19 a.u., p = 0.003, and 0.71 ± 0.08 a.u. vs. 1.26 ± 0.42 a.u., p = 0.03, respectively) as were the levels of IL-17 in culture supernatants of OLE + EC-LPS treated colonic samples (21.16 ± 8.64 pg/mL vs. 40.67 ± 9.24 pg/mL, p = 0.01). Histologically, OLE-treated colonic samples showed an amelioration of inflammatory damage with reduced infiltration of CD3, CD4, and CD20 cells, while CD68 numbers increased. The anti-inflammatory activity of OLE was demonstrated in colonic biopsies from UC patients. These new data support a potential role of OLE in the treatment of UC.

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Chemopreventive effect of oleuropein in colitis‐associated colorectal cancer in c57bl/6 mice

Abstract: Oleuropein as a dietary supplementation could be a promising protective agent against colitis-associated CRC.

Cited by 102 publications

References 38 publications

Oleuropein is a Powerful Sensitizer of Doxorubicin-mediated Killing of Prostate Cancer Cells and Exerts Its Action via Induction of Autophagy

Oleuropein is a Powerful Sensitizer of Doxorubicin-mediated Killing of Prostate Cancer Cells and Exerts Its Action via Induction of Autophagy

Antitumor Perspectives of Oleuropein and Its Metabolite Hydroxytyrosol: Recent Updates

Oleuropein Decreases Cyclooxygenase-2 and Interleukin-17 Expression and Attenuates Inflammatory Damage in Colonic Samples from Ulcerative Colitis Patients

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