Chemopreventive effect of Indigofera linnaei extract against diethylnitrosamine induced hepatocarcinogenesis in rats

Abstract

Indigofera linnaei Ali., has been widely used in folk medicine to treat inflammation, liver disorders and swelling of stomach. Earlier studies showed that methanolic extract of Indigofera linnaei (MEIL) possess antioxidant, free radical scavenging, antitumour, anti-inflammatory and analgesic activities but its effect on chemoprevention on chemical induced carcinogenesis have not been studied. Hence the present study is aimed to investigate chemopreventive efficacy of MEIL against diethylnitrosamine (DEN) induced phenobarbital promoted rat liver carcinoma model. Liver tumour was induced in rats by intraperitoneal administration of DEN (200 mg/kg). After two weeks, the carcinogenic effect was promoted by phenobarbital given through drinking water for 16 successive weeks. MEIL (200 and 400 mg/kg/day) and silymarin (200 mg/kg/day) were administered orally for entire study period. After the experimental period, changes in body weight, liver weight, relative liver weight, tumour incidence, antioxidant, serum hepatic parameters, serum specific tumour markers, nucleic acid and protein content were analysed. MEIL treatment significantly increased the body weight, reduced the liver weight and tumour incidence, restored the altered serum hepatic parameters, down-regulated the serum tumour markers such as alpha fetoprotein, carcinoembryonic antigen, vasculoendothelial growth factor and tumor necrosis factor-α. In addition, treatment with MEIL restored the antioxidant enzyme pool and significantly reduced the lipid peroxidation in carcinogen treated animals. MEIL treatment also reduced the elevated levels of nucleic acid levels and restored the protein content in liver tissues. The results suggested that MEIL would be a potent chemopreventive agent inhibiting chemically induced hepatic cancer.

Newcastle and Doxorubicin attenuated against diethylnitrosoamine as anti-proliferative and immunosuppresor effects which induced hepatocellular carcinoma

“…The obtained results revealed that, DEN induced HCC showed upregulation in serum IL-6 and TNF-α. Similar results were obtained by (Kumar et al, 2016) who confirmed that, TNF-α level significantly upregulatation in DEN treated animals. Moreover, (Song et al, 2013) showed that, DEN-induced HCC increased TNF-α, such tumor necrosis factor alpha (TNF-α) is pro-inflammatory cytokines produced by macrophages and it plays an important role under tumor conditions (Lutsiak et al, 2005).…”

Camel (Camelus Dromedarius) Milk Antibodies Ameliorated Diethylnitrosamine-Induced Hepatocellular Carcinoma in Wistar Rats

“…Oppositely, HCC group showed diffused white nodules on the liver with a pale appearance and liver photomicrograph stained with H&E showed loss of normal architecture of hepatocytes, neoplastic hepatocytes with pleomorphism, increased nuclear to cytoplasmic ratio (N/C), nuclear atypia and hyperchromasia (black arrows). Areas of hemorrhagic necrosis (H) invaded by mononuclear cellular infiltration (K; kupffer cells) are also observed (Figure 7), this was in accordance with previous studies (22,64,65). Figure 8 showed no gross lesions on the liver surface of HCC-bearing rats treated with CM-Ig and its photomicrograph stained with H&E showed diffuse vacuolization of hepatocytes.…”

“…Hepatocellular carcinoma (HCC) is one of the most malignant liver tumours with high aggressive growth behaviour, elevated mortality, and increased rate of recurrence (21). HCC ranks the fifth of the most widespread malignancies worldwide and the second main cause of cancer-associated deaths (22). Its incidence in Egypt is increased rapidly due to viral hepatitis infections (HCV and HBV) that make it a major health problem (23).…”

Pomegranate juice and punicalagin-mediated chemoprevention of hepatocellular carcinogenesis via regulating miR-21 and NF-κB-p65 in a rat model

“…Liver cancer may produce complex metabolic disturbances resulting in rapid loss of body weight and tissue emaciation [ 36 ]. This study reported that the HCC control rats had a significant reduction in body and liver weight, with a significant increase in relative liver weight, as compared to the control group.…”