Chemopreventive effect of Callistemon citrinus (Curtis) Skeels against colon cancer induced by 1,2-dimethylhydrazine in rats

López-Mejía, Alejandro; Ortega-Pérez, Luis Gerardo; Godínez-Hernández, Daniel; Nateras-Marín, Blanca; Meléndez‐Herrera, Esperanza; Ríos-Chávez, Patricia

doi:10.1007/s00432-019-02905-3

Cited by 19 publications

(16 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This demonstrated that Nrf-2 activation is an auxiliary therapy, possibly useful for colitis and colon cancer patients. The augmented sternness of colonic damage in DMH group animals may be correlated with a decrease in phase II detoxification enzymes, as reported previously [34]. In Nrf-2-deficient mice, proinflammatory modulators like interleukin 1β (IL-1β), interleukin 6 (IL-6), and tumor necrosis factor α (TNF-α) were prominently elevated as compared with the colon tissues of wild-type Nrf-2 mice [33,35].…”

Section: Discussionsupporting

confidence: 72%

Piperine Regulates Nrf-2/Keap-1 Signalling and Exhibits Anticancer Effect in Experimental Colon Carcinogenesis in Wistar Rats

Rehman

Rashid

Arafah

et al. 2020

Biology

View full text Add to dashboard Cite

Colon cancer is the most common cancer in men and women globally, killing millions of people annually. Though there widespread development has been made in the management of colorectal cancer, still there is an urgent need to find novel targets for its effective treatment. Piperine is an alkaloid found in black pepper having anticancer, anti-inflammatory activities, safe and nutritive for human consumption. Nuclear factor-erythroid 2–kelch-like ECH-associated protein 1(Nrf-2/Keap-1)/Heme-oxygenase1 (HO-1) signaling pathway plays a vital part in shielding cells from intracellular oxidative stress and inflammation. A potential cross-talk between the Nrf-2 and NF-κB pathways is recognized during cancerous growth and expansion. We studied this pathway extensively in the present study to discover novel targets in the prevention of chemically induced colon cancer with piperine to simulate human colon cancer pathology. Animals were divided into four groups. Groups1 and 2 were used as a negative control and positive control where 1,2–Dimethylhydrazine, DMH was administered in group 2, while group 3 and 4 were prevention groups where piperine at two different doses was given two weeks prior to DMH and continued until end of experiment. We found that piperine inhibited NF-κB by the activation of Nrf-2, blocking downstream inflammatory mediators/cytokines (TNF-α, IL-6, IL-1β, Cox-2, PGE-2, iNOS, NO, MPO), triggering an antioxidant response machinery (HO-1, NQO-1, GSH, GR, GPx, CAT, SOD), scavenging ROS, and decreasing lipid peroxidation. Histological findings further validated our molecular findings. It also downregulates CEA, MDF and ACF, markers of precancerous lesions in colon, alleviates infiltration of mast cells and depletes the mucous layer. Our results indicate that piperine may be an effective molecule for the prophylactic treatment of colon carcinogenesis by targeting the NF-κB/Nrf-2/Keap-1/HO-1 pathway as a progressive strategy in the preclusion and effective treatment of colorectal cancer.

show abstract

Section: Discussionsupporting

confidence: 72%

Piperine Regulates Nrf-2/Keap-1 Signalling and Exhibits Anticancer Effect in Experimental Colon Carcinogenesis in Wistar Rats

Rehman

Rashid

Arafah

et al. 2020

Biology

View full text Add to dashboard Cite

show abstract

“…42 In addition, DMH showed no signicant effect on calorie intake between groups. 43 However, in the current study, administration of inulin, L. casei or inulin + L. casei together with DMH signicantly decreased the body weight compared to either the control or the DMH groups. It is worthy to mention that the effect of administration of pre-, proor synbiotic formulations on the body weight in literature is controversial so far.…”

Section: Discussioncontrasting

confidence: 68%

Modulation of JNK-1/ β-catenin signaling byLactobacillus casei, inulin and their combination in 1,2-dimethylhydrazine-induced colon cancer in mice

et al. 2019

View full text Add to dashboard Cite

Colon cancer is a complex disease that involves numerous genetic alterations that change the normal colonic mucosa into invasive adenocarcinoma. In the current study, the protective effects of inulin (prebiotic), Lactobacillus casei (L. casei, probiotic) and their combination (synbiotic) on 1,2dimethylhydrazine (DMH)-induced colon cancer in male Swiss mice were evaluated. Animals were divided into: Control group, DMH-treated group, DMH plus inulin, DMH plus L. casei and DMH plus inulin plus L. casei-treated groups. Fecal microbiome analysis, biochemical measurements, histopathological examination of the colon tissues, immunostaining and Western blotting analysis of bcatenin, GSK3b and JNK-1 were performed. The prebiotic-, probiotic-and synbiotic-treated groups showed decreased levels of carcinoembryonic antigen and a lower number of aberrant crypt foci compared to the DMH-treated group with the synbiotic group exhibiting a superior effect. Furthermore, all treatments showed a body weight-reducing effect. Administration of inulin, L. casei or their combination increased the expression level of phospho-JNK-1 while they decreased the expression level of b-catenin and phospho-GSK3b. Remarkably, L. casei treatment resulted in enrichment of certain beneficial bacterial genera i.e. Akkermansia and Turicibacter. Therefore, administration of L. casei and inulin as a synbiotic combination protects against colon cancer in mice.

show abstract

“…Therefore, demonstrating that Nrf-2 activation is an auxiliary therapy possibly for colitis and colon cancer patients. The augmented sternness of colonic damage in DMH group animals may be correlated with decrease in phase II detoxi cation enzymes as reported previously [24]. In Nrf-2 de cient mice, proin ammatory modulators like interleukin 1β (IL-1β), interleukin 6 (IL-6), and tumor necrosis factor α (TNF-α) were prominently elevated as compared with the colon tissues of wild-type Nrf-2 mice [23,25].…”

Section: Discussionsupporting

confidence: 71%

Anti-Cancer Activity of Piperine Against Colon Carcinogenesis Via Modulation of NF-κB/Nrf-2/Keap1/HO-1 Signalling Pathways

Rehman¹,

Rashid²,

Arafah³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Colon cancer is the most common cancer in men and women globally, killing millions of people annually. Though widespread development has been made in the management of colorectal cancer, still there is an urgency to find novel targets for effective treatment of these patients. Piperine is an alkaloid found in black pepper having anticancer, anti-oxidant and anti-inflammatory activities. Nuclear factor-erythroid 2–kelch-like ECH-associated protein 1(Nrf-2/Keap1)/ Heme-oxygenase1 (HO-1) signaling pathway plays a vital part in shielding cells from the injury of intracellular oxidative stress and inflammation. A potential cross-talk between Nrf-2 and NF-κB pathways is recognized during cancerous growth and expansion. We studied this pathway extensively in the present study to discover novel targets in prevention of chemically induced colon cancer with piperine.Results: We found that NF-κB inhibition occurs by the activation of Nrf-2 blocks downstream inflammatory mediators/cytokines (TNF-α, IL-6, IL-1β, Cox-2, PGE2, iNOS, NO, MPO) and triggers antioxidant response machinery (HO-1, NQO-1 GSH, GR, GPx, CAT, SOD), scavenges ROS, decreases lipid peroxidation. Histological findings further validated our defensive effects of piperine on colon carcinogenesis. Piperine down-regulates CEA, MDF and ACF, markers of precancerous lesions in colon.Conclusion: Our results indicate that piperine may be an effective molecule for the prophylactic treatment of colon carcinogenesis by targeting NF-κB/Nrf-2/Keap1/HO-1 pathway as a progressive strategy in preclusion and effective treatment of colorectal cancer.

show abstract

Chemopreventive effect of Callistemon citrinus (Curtis) Skeels against colon cancer induced by 1,2-dimethylhydrazine in rats

Cited by 19 publications

References 48 publications

Piperine Regulates Nrf-2/Keap-1 Signalling and Exhibits Anticancer Effect in Experimental Colon Carcinogenesis in Wistar Rats

Piperine Regulates Nrf-2/Keap-1 Signalling and Exhibits Anticancer Effect in Experimental Colon Carcinogenesis in Wistar Rats

Modulation of JNK-1/ β-catenin signaling byLactobacillus casei, inulin and their combination in 1,2-dimethylhydrazine-induced colon cancer in mice

Anti-Cancer Activity of Piperine Against Colon Carcinogenesis Via Modulation of NF-κB/Nrf-2/Keap1/HO-1 Signalling Pathways

Contact Info

Product

Resources

About