Chemopreventive Action by Ethanol-extracted Brazilian Green Propolis on Post-initiation Phase of Inflammation-associated Rat Colon Tumorigenesis

Doi, Kenichiro; Fujioka, Masaki; Sokuza, Yui; Ohnishi, Mariko; Gi, Min; Takeshita, Masanori; Kumada, Kenji; Kakehashi, Anna; Wanibuchi, Hideki

doi:10.21873/invivo.11044

Cited by 20 publications

(15 citation statements)

References 44 publications

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“…Ethanol extraction has been described as the most suitable medium for the extraction of biologically active phenolic components from propolis [96–99]. In addition, the industrial extraction method commonly used to obtain biocompounds from propolis is conventional extraction (ethanolic or hydroethanolic extraction), where the sample can be submerged in a solvent for days, weeks, or months, which requires an enormous amount of time when extracting on an industrial scale (usually at room temperature) [1,100,101]. Thus, the findings of this study show that the use of ultrasound technology as a treatment during the extraction process is a viable alternative for obtaining antioxidant compounds from propolis in a short period of time when compared with the applied conventional extraction (ethanolic extraction for 7 days) that is usually employed by industry.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of the antioxidant profile and cytotoxic activity of red propolis extracts from different regions of northeastern Brazil obtained by conventional and ultrasound-assisted extraction

et al. 2019

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Propolis is a complex mixture of resinous and balsamic material collected from the exudates of plants, shoots, and leaves by bees. This study evaluated red propolis extracts obtained by conventional (ethanolic) extraction and ultrasound-assisted extraction of six samples from different regions of northeastern Brazil. The total phenolic compounds and flavonoids, in vitro antioxidant activity, concentration of formononetin and kaempferol and the cytotoxicity against four human tumor cell lines were determined for all twelve obtained extracts. Significant variations in the levels of the investigated compounds were identified in the red propolis extracts, confirming that the chemical composition varied according to the sampling region. The extraction method used also influenced the resulting propolis compounds. The highest concentration of the compounds of interest and the highest in vitro antioxidant activity were exhibited by the extracts obtained from samples from state of Alagoas. Formononetin and kaempferol were identified in all samples. The highest formononetin concentrations were identified in extracts obtained by ultrasound, thus indicating a greater selectivity for the extraction of this compound by this method. Regarding cytotoxic activity, for the HCT-116 line, all of the extracts showed an inhibition of greater than 90%, whereas for the HL-60 and PC3 lines, the minimum identified was 80%. In general, there was no significant difference (p>0.05) in the antiproliferative potential when comparing the extraction methods. The results showed that the composition of Brazilian red propolis varies significantly depending on the geographical origin and that the method used influences the resulting compounds that are present in propolis. However, regardless of the geographical origin and the extraction method used, all the red propolis samples studied presented great biological potential and high antioxidant activity. Furthermore, the ultrasound-assisted method can be efficiently applied to obtain extracts of red propolis more quickly and with high concentration of biomarkers of interest.

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Section: Resultsmentioning

confidence: 99%

Evaluation of the antioxidant profile and cytotoxic activity of red propolis extracts from different regions of northeastern Brazil obtained by conventional and ultrasound-assisted extraction

et al. 2019

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“…Thus, Doi et al evaluated the effects of ethanolic and water-extracts produced from Brazilian green propolis in the model of inflammation-associated rat colon carcinogenesis (1,2-dimethylhydrazine plus DSS treatment). The authors showed that the ethanol based extract exerted its anticancer effects through the suppression of inflammatory factors, such as tumor necrosis factor (TNF- α ) and iNOS [ 70 ].…”

Section: Propolis In the Therapeutic Management Of Ulcerative Colimentioning

confidence: 99%

Propolis and Its Potential to Treat Gastrointestinal Disorders

Silva

Souza

Jaouni

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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There are a number of disorders that affect the gastrointestinal tract. Such disorders have become a global emerging disease with a high incidence and prevalence rates worldwide. Inflammatory and ulcerative processes of the stomach or intestines, such as gastritis, ulcers, colitis, and mucositis, afflict a significant proportion of people throughout the world. The role of herbal-derived medicines has been extensively explored in order to develop new effective and safe strategies to improve the available gastrointestinal therapies that are currently used in the clinical practice. Studies on the efficacy of propolis (a unique resinous aromatic substance produced by honeybees from different types of species of plants) are promising and propolis has been effective in the treatment of several pathological conditions. This review, therefore, summarizes and critiques the contents of some relevant published scientific papers (including those related to clinical trials) in order to demonstrate the therapeutic value of propolis and its active compounds in the treatment and prevention of gastrointestinal diseases.

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“…Several studies indicated that chemical carcinogens induced colon cancer progression primarily by causing damage to the colonic mucosa [35]. ACF mainly appear in the distal colon at an early stage and expand to the proximal colon during advanced progression, and thus there is a relatively high number of ACF in the middle and distal colons [36,37].…”

Section: Discussionmentioning

confidence: 99%

Synbiotic Combination of Djulis (Chenopodium formosanum) and Lactobacillus acidophilus Inhibits Colon Carcinogenesis in Rats

et al. 2019

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Djulis is a functional grain containing prebiotic dietary fiber, which has an anti-cancer potential. This study examined the preventive effect of djulis alone or in combination with Lactobacillus acidophilus on colon carcinogenesis induced by 1,2-dimethylhydrazine (DMH) and dextran sulfate sodium (DSS). Rats were divided into five groups and fed B (AIN-93G, blank), C (AIN-93G, control), D (10% djulis), DLA (10% djulis plus 5 × 106 cfu L. acidophilus/g), and DHA (10% djulis plus 5 × 107 cfu L. acidophilus/g) diets, respectively. All rats except for those in group B received three doses of DMH (40 mg/kg) by intraperitoneal injection and 3% DSS in drinking water. After 10 weeks of feeding, the colon was analyzed for precancerous lesions and biomarkers. DMH and DSS treatment induced aberrant crypt foci (ACF), especially in the distal colon. D, DLA, and DHA significantly reduced the numbers of total ACF, sialomucin-producing ACF (SIM-ACF), and mucin-depleted foci (MDF) in the distal colon compared to C. Additionally, DLA and DHA further downregulated the expressions of proliferating cell nuclear antigen (PCNA) and cyclooxygenase-2 (COX-2) and regulated apoptosis-related proteins. These results suggest that synbiotic combination of djulis and L. acidophilus shows the best inhibitory effect on colon carcinogenesis via regulation of proliferative, inflammatory, and apoptotic pathways.

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Chemopreventive Action by Ethanol-extracted Brazilian Green Propolis on Post-initiation Phase of Inflammation-associated Rat Colon Tumorigenesis

Abstract: Abstract. Background

Cited by 20 publications

References 44 publications

Evaluation of the antioxidant profile and cytotoxic activity of red propolis extracts from different regions of northeastern Brazil obtained by conventional and ultrasound-assisted extraction

Evaluation of the antioxidant profile and cytotoxic activity of red propolis extracts from different regions of northeastern Brazil obtained by conventional and ultrasound-assisted extraction

Propolis and Its Potential to Treat Gastrointestinal Disorders

Synbiotic Combination of Djulis (Chenopodium formosanum) and Lactobacillus acidophilus Inhibits Colon Carcinogenesis in Rats

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