Chemoprevention of mammary carcinogenesis in female rats by rofecoxib

Kubatka, Peter; Ahlers, I; Ahlersová, E; Adámeková, E.; Luk, Pauline; Bojková, Bianka; Marková, Martina

doi:10.1016/j.canlet.2003.08.006

Cited by 34 publications

(31 citation statements)

References 11 publications

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“…COX-2 overexpression was previously reported to correlate with invasive breast cancer (47). Forced overexpres-sion of COX-2 likewise induces mammary tumorigenesis in multiparous mice through a PGE 2 -EP2 pathway (48), whereas inhibition of COX-2 significantly suppresses mammary tumorigenesis in rats treated with 7,12-dimethylbenzanthracene and N-methyl-N-nitrosourea (49,50). However, the expression of mPGES1 may be independent of COX-2 (13,51).…”

Section: Discussionmentioning

confidence: 98%

Krüppel-like Factor 5 Transcription Factor Promotes Microsomal Prostaglandin E2 Synthase 1 Gene Transcription in Breast Cancer

Xia

Wang

Chen

et al. 2013

Journal of Biological Chemistry

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Section: Discussionmentioning

confidence: 98%

Krüppel-like Factor 5 Transcription Factor Promotes Microsomal Prostaglandin E2 Synthase 1 Gene Transcription in Breast Cancer

Xia

Wang

Chen

et al. 2013

Journal of Biological Chemistry

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“…COX-2 is the rate-limiting enzyme in the conversion of arachidonic acid to PGE 2 which is a pro-inflammatory mediator present in the inflammatory site [17,18]. Persistence inflammation and continuous production of COX-2 has been linked to development of cancer and autoimmune disorders [19,20]. In this study, we found that loquat tea could attenuate the production of PGE 2 and COX-2 induced by LPS.…”

Section: Discussionmentioning

confidence: 60%

Antioxidant and anti-inflammatory activities of loquat (Eriobotrya japonica) tea

Zar

Sakao²,

Hashimoto

et al. 2013

FFHD

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Background: Fresh loquat leaves contain several kinds of flavonoids and have been reported to have preventive effects against some human diseases such as diabetes, coughs and ulcers,. Recently, fresh loquat leaves in Japan were processed to a beverage, called loquat tea, after the fresh leaves are roasted at 350C for 30 minutes. However, the scientific evidence supporting the functions of these processed leaves is still minimal.Objective: The aim of this study is to investigate the antioxidant and anti-inflammatory activities of roasted loquat tea extract (LTE) in vitro and in culture cells. Methods:Bioactive fractions of LTE were separated by column chromatograph. Antioxidant activities were determined by DPPH and ROS assay. Pro-inflammatory mediators cyclooxygenase-2 (COX-2) and prostaglandin E 2 (PGE 2 ) were determined by Western blot and ELISA assay, respectively. Chemical quantification and characterization were analyzed by HPLC, FR-IR, and NMR. Phenolic content was measured by Folin-Ciocalteu assay. Results:The results showed that loquat tea extract (LTE) possessed stronger DPPH scavenging activity than fresh. Cellular data revealed that LTE inhibited the production of reactive oxygen species (ROS), and further suppressed the production of COX-2 and PGE 2 in lipopolysaccharide (LPS)-activated RAW 264.7 cells. Chemical quantification and characterization data indicated that LTE contained new bioactive phenolic components that were produced from the roasting processes of fresh loquat leaves. Conclusions: Loquat tea made from roasted loquat leaves contained new bioactive phenolic compounds that contribute to its antioxidant and anti-inflammatory activities.

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“…In another study of our group, preventive effect of dietary administered rofecoxib in NMU-induced mammary carcinogenesis in female Sprague-Dawley rats was confirmed by decreased tumor incidence and frequency at both doses we used (0.01 mg g −1 , 0.05 mg g −1 diet). Latency period was prolonged by 0.5% and 10%, respectively (Kubatka et al 2003). Our next study focused on prevention of NMU-induced mammary carcinogenesis by etoricoxib administered in a diet to female Sprague-Dawley rats.…”

Section: Discussionmentioning

confidence: 99%

“…Our group proved the chemopreventive effects of indomethacin administered in drinking water (Môci-ková-Kalická et al 2001), nimesulide applied subcutaneously (Kubatka et al 2002), rofecoxib (Kubatka et al 2003), etoricoxib (Orendáš et al 2007), and celecoxib (Orendáš et al 2009), all administered in the diet to female Sprague-Dawley rats with chemically-induced mammary tumors. Generally, at least 90% of mammary tumors showed malignant features, cribriform carcinoma in situ and invasive adenocarcinomas prevailed; the malignant/benign tumor ratio maintained regardless of oncostatic efficacy (Orendáš et al 2009).…”

Section: Introductionmentioning

confidence: 89%

Chemopreventive effect of diclofenac on mammary carcinogenesis in Sprague-Dawley rats

et al. 2013

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Chemopreventive effect of non-steroidal antiinflammatory drugs (NSAIDs) in mammary carcinogenesis was reported in several studies. In this study, the effect of a nonselective cyclooxygenase inhibitor diclofenac (DICLO) in the prevention of N-methyl-N-nitrosourea (NMU)-induced mammary carcinogenesis in Sprague-Dawley female rats was evaluated. NMU was administered to animals intraperitoneally in two doses of 50 mg kg −1 b.w. within postnatal days 42-48. In experiment A (short-term administration), DICLO was administrated intramuscularly (5 mg kg −1 b.w.) every other day, starting 3 days before and for subsequent 25 days after first NMU injection. In experiment B (long-term administration), DICLO was administered in tap water (0.01 mg ml −1 ) continually, starting 7 days before and for subsequent 22 weeks after first NMU dose. The study was terminated 22 weeks after the first dose of NMU in both experiments. After DICLO treatment, tumor frequency per group was reduced in both variants of drug administration: in experiment A by 38% and in experiment B by 39.5%. Moreover, DICLO decreased tumor incidence by 11.5% and delayed tumor latency by 14 days in experiment B. In our preventive-curative experiments DICLO decreased some parameters of NMU-induced rat mammary carcinogenesis, mainly the tumor frequency.

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Chemoprevention of mammary carcinogenesis in female rats by rofecoxib

Cited by 34 publications

References 11 publications

Krüppel-like Factor 5 Transcription Factor Promotes Microsomal Prostaglandin E2 Synthase 1 Gene Transcription in Breast Cancer

Krüppel-like Factor 5 Transcription Factor Promotes Microsomal Prostaglandin E2 Synthase 1 Gene Transcription in Breast Cancer

Antioxidant and anti-inflammatory activities of loquat (Eriobotrya japonica) tea

Chemopreventive effect of diclofenac on mammary carcinogenesis in Sprague-Dawley rats

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