Chemoprevention of Leishmaniasis: In - vitro antiparasitic activity of dibenzalacetone, a synthetic curcumin analog leads to apoptotic cell death in Leishmania donovani

Chauhan, Indira Singh; Rao, Gita Subba; Shankar, Jai; Chauhan, Lalit Kumar Singh; Kapadia, Govind J.; Singh, Neeloo

doi:10.1016/j.parint.2018.06.004

Cited by 44 publications

(29 citation statements)

References 48 publications

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“…Then, intracellular amastigote activity was performed and showed that C. longa essential oil not only maintained the antileishmanial activity observed against promastigote form but also presented higher efficacy against intracellular amastigote. Trans-dibenzalacetone, a synthetic monoketone analog of curcumin, demonstrated antiproliferative effect on the intracellular amastigotes of L. donovani (IC 50 7.43 ± 1.88 μ g/mL) that also was significantly lower than the IC 50 value determined for promastigotes (17.80 ± 1.42 μ g/mL) [43]. Selectivity index demonstrated the selective antileishmanial activity of C. longa essential oil for intracellular amastigote with similar value of trans-dibenzalacetone, SI value of 15.34 for intracellular amastigotes against the BALB/c mouse cell line J774A.1 [43].…”

Section: Discussionmentioning

confidence: 99%

“…Mechanisms of direct action on Leishmania have already been described for the analog of curcumin trans-dibenzalacetone. It alters the general ultrastructure and the mitochondrial physiology of the parasite and triggers apoptotic cell death in L. donovani [43]. Macrophage activation is one of the more important mechanisms of indirect action, as induction of reactive oxygen species and reactive oxygen nitrogen species.…”

Section: Discussionmentioning

confidence: 99%

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Cinnamomum zeylanicum, Origanum vulgare, and Curcuma longa Essential Oils: Chemical Composition, Antimicrobial and Antileishmanial Activity

Teles

Rosa

Mouchrek

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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The resistance mechanisms of bacteria and protozoans have evidenced the need of discover new compounds with potential pharmaceutical activity against pathogenic microorganisms. Medicinal plants have been for centuries a promising alternative as sources of new drugs. The objective of this work was to evaluate the chemical composition, antimicrobial and antileishmanial activities of Cinnamomum zeylanicum, Origanum vulgare, and Curcuma longa essential oils. Chemical analysis was performed by gas chromatography-mass spectrometry. Antimicrobial activity was performed by disk diffusion and minimum inhibitory concentration (MIC) test. Antileishmanial activity was performed against antipromastigote and intracellular amastigote of Leishmania amazonensis. Cytotoxic and nitrite production were realized in BALB/c peritoneal macrophages. The major compounds of the essential oils were cinnamic aldehyde (46.30%) in C. zeylanicum, cis-p-menth-2-en-1-ol (33.88%) and linalyl acetate (13.90%) in O. vulgare, and turmerone (55.43%) in C. longa. The MIC showed significant antimicrobial activity of C. longa essential oil against S. aureus (83.3 ± 14.43 µg/mL). Antipromastigote activity showed IC50 values >500 µg/mL to C. zeylanicum, 308.4 ± 1.402 µg/mL to O. vulgare, and 405.5 ± 1.119 µg/mL to C. longa essential oil. Activity against intracellular amastigote of L. amazonensis showed IC50 of 63.3 ± 1.369 µg/mL and cytotoxic was not observed, resulting in selectivity index higher than 15.79 to parasite. C. longa essential oil decreased nitrite production in peritoneal macrophages, but not in Leishmania-infected cells. The chemical composition of the three essential oils is directly associated to its potential biological action, as the antimicrobial activity. C. longa presented a potent antileishmanial activity against promastigote and intracellular amastigote of L. amazonensis, although this activity is not linked to nitric oxide, since C. longa essential oil inhibits its production.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Cinnamomum zeylanicum, Origanum vulgare, and Curcuma longa Essential Oils: Chemical Composition, Antimicrobial and Antileishmanial Activity

Teles

Rosa

Mouchrek

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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“…In this study, Curcuma longa L. (Zingiberaceae) was cited as a natural remedy for leishmaniasis only once. However, the leishmanicidal activity of curcumins has been recorded since 2000 ( Rasmussen et al, 2000 ; Saleheen et al, 2002 ), and further works demonstrated that synthetic derivatives also present high activity at eliminating extra- and intracellular parasites ( Gomes et al, 2002 ; Chauhan et al, 2018 ; Teles et al, 2019 ), and such activity may be related to programmed cell death in L. donovani ( Chauhan et al, 2018 ). Despite these advances, in vivo studies with Curcuma longa L. or curcumin are scarce in the literature.…”

Section: Plants Recommended For the Treatment Of Leishmaniasis By Traditional Communities Worldwidementioning

confidence: 99%

The Potential of Traditional Knowledge to Develop Effective Medicines for the Treatment of Leishmaniasis

et al. 2021

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Leishmaniasis is a neglected tropical disease that affects people living in tropical and subtropical areas of the world. There are few therapeutic options for treating this infectious disease, and available drugs induce severe side effects in patients. Different communities have limited access to hospital facilities, as well as classical treatment of leishmaniasis; therefore, they use local natural products as alternative medicines to treat this infectious disease. The present work performed a bibliographic survey worldwide to record plants used by traditional communities to treat leishmaniasis, as well as the uses and peculiarities associated with each plant, which can guide future studies regarding the characterization of new drugs to treat leishmaniasis. A bibliographic survey performed in the PubMed and Scopus databases retrieved 294 articles related to traditional knowledge, medicinal plants and leishmaniasis; however, only 20 were selected based on the traditional use of plants to treat leishmaniasis. Considering such studies, 378 quotes referring to 292 plants (216 species and 76 genera) that have been used to treat leishmaniasis were recorded, which could be grouped into 89 different families. A broad discussion has been presented regarding the most frequent families, including Fabaceae (27 quotes), Araceae (23), Solanaceae and Asteraceae (22 each). Among the available data in the 378 quotes, it was observed that the parts of the plants most frequently used in local medicine were leaves (42.3% of recipes), applied topically (74.6%) and fresh poultices (17.2%). The contribution of Latin America to studies enrolling ethnopharmacological indications to treat leishmaniasis was evident. Of the 292 plants registered, 79 were tested against Leishmania sp. Future studies on leishmanicidal activity could be guided by the 292 plants presented in this study, mainly the five species Carica papaya L. (Caricaceae), Cedrela odorata L. (Meliaceae), Copaifera paupera (Herzog) Dwyer (Fabaceae), Musa × paradisiaca L. (Musaceae), and Nicotiana tabacum L. (Solanaceae), since they are the most frequently cited in articles and by traditional communities.

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“…Chauhan et al found that DBA (dibenzalacetone) can lead to apoptosis off cells in Leishmania donovani ( L. donovani ) via the activation of the MAPK cascades (Table 2) [11].…”

Section: Research Directions and Mapk Mechanism Of Action In Parasmentioning

confidence: 99%

“…For instance, Erika Pellegrini et al found that the intracellular parasite Toxoplasma gondii has an ability to deliver a protein, GRA24, into cells, which establishes an interaction with a host protein, p38αMAPK(MAPK14). Consequently this interaction results in activation and nuclear translocation of the host kinase, which triggers an inflammatory response [11]. In addition, MAPK is regulated through the activation of three family members: MAPKKK/MEKK/MKKK, MAPKK/MEK/MKK, and MAPK [5].…”

Section: Introductionmentioning

confidence: 99%

The MAPK Signaling Pathways as a Novel Way in Regulation and Treatment of Parasitic Diseases

et al. 2019

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Few major advances in fighting parasitic diseases have been made in China since the development of new methods for prevention, control, and elimination. However, the proportion of immunocompromised individuals has increased due to the growth of chronic diseases, population aging, and more frequent cases of patients with AIDS and cancer. All these problems can promote development of parasitic infections, which is commonly associated with manipulation of host signaling pathways and the innate immune system. Mitogen-activated protein kinase (MAPK) signaling pathways are evolutionarily conserved in metazoan organisms, which play critical roles in the cell cycle, gene expression, growth, differentiation, apoptosis, and parasite–host interactions. Recent discoveries of the MAPK components involved in activation, regulation, and signal transduction appeared to be promising for the diagnosis, prevention, and treatment of parasitic diseases in the future. This review summarizes the involvement and critical role of the MAPK family in parasitic disease development and maintenance in the host. Moreover, it highlights recent studies concerning the mechanisms and novel drug development for inhibition and regulation of MAPK pathways in order to prevent parasitic disease. In addition, we discuss some antigenic proteins as prospective inhibitory molecules or vaccines for the regulation and control of MAPK signaling involved in parasite physiological activity.

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Chemoprevention of Leishmaniasis: In - vitro antiparasitic activity of dibenzalacetone, a synthetic curcumin analog leads to apoptotic cell death in Leishmania donovani

Cited by 44 publications

References 48 publications

Cinnamomum zeylanicum, Origanum vulgare, and Curcuma longa Essential Oils: Chemical Composition, Antimicrobial and Antileishmanial Activity

Cinnamomum zeylanicum, Origanum vulgare, and Curcuma longa Essential Oils: Chemical Composition, Antimicrobial and Antileishmanial Activity

The Potential of Traditional Knowledge to Develop Effective Medicines for the Treatment of Leishmaniasis

The MAPK Signaling Pathways as a Novel Way in Regulation and Treatment of Parasitic Diseases

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