Chemometrics applied to the analysis of induced phytochelatins in Hordeum vulgare plants stressed with various toxic non-essential metals and metalloids

Dago, Àngela; González, Inmaculada; Ariño, Cristina; Dı́az-Cruz, José Manuel; Esteban, Miquel

doi:10.1016/j.talanta.2013.09.058

Cited by 29 publications

(20 citation statements)

References 22 publications

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“…Moreover, in previous studies Hordeum vulgare plants cultured with mercury or other heavy metals have been considered for the determination of the induced PCs and the Hg-PC complexes formed with HPLC with amperometric detection. 35,36 For the present study, wild asparagus (Asparagus acutifolius) was selected, because it is a typical evergreen perennial plant that grows naturally in the mining zone of the Almadeń district. 23 Several zones with different Hg concentrations were chosen, and aerial and underground parts of plants from these zones were analyzed separately to determine the total concentration of Hg, as well as the concentrations of PCs and their Hg complexes.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Mercury Stress in Plants from the Almadén Mining District by Analysis of Phytochelatins and Their Hg Complexes

Dago

González

Ariño

et al. 2014

Environ. Sci. Technol.

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To evaluate plant response to Hg stress, glutathione, phytochelatins, and their Hg complexes were analyzed using HPLC with amperometric detection in samples of Asparagus acutifolius grown in the Almadén mining district (Ciudad Real, Spain), one of the most Hg-contaminated sites in the world. Soils of the Almadén mining district, and specifically from the Almadenejos zone, are highly contaminated, with some zones having values above 4,000 μg Hg g(-1) soil. Although soils have an extremely high concentration of mercury, generally less than 2% is available for plants, as is shown by various soil extractions simulating bioavailability. In plants, Hg concentration increases depending on the content of Hg in soils. In addition, Hg levels in roots are higher than in aerial parts, which is a strategy of plants for protecting their more sensitive aerial parts from the deleterious effects of metal stress. The total content of phytochelatins (PCs) and their complexes are directly related with the amount of mercury in soils. These findings highlight the important role of thiol compounds and their metal complexes in capturing and fixing Hg from soils, giving plants the capacity to deal with the heavy metal toxicity of polluted soils.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Mercury Stress in Plants from the Almadén Mining District by Analysis of Phytochelatins and Their Hg Complexes

Dago

González

Ariño

et al. 2014

Environ. Sci. Technol.

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“…, and Zn 2+ [27], and the best inducer of PCs is cadmium [28]. It was reported that arabidopsis mutants lacking PC synthase had a definite cadmium-sensitive phenotype, but copper tolerance was not affected [29].…”

Section: +mentioning

confidence: 99%

Gene Cloning, Protein Expression and Functional Analysis of a type 3 Metallothionein with Bioaccumulation Potential from Sonneratia alba

Niu¹,

Li²,

Yi³

et al. 2018

Pol. J. Environ. Stud.

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Sonneratia alba (S. alba) is a mangrove species grown in brackish water of tropical and subtropical regions. Due to its unique environment, it has evolved various mechanisms for modulating salt and metal levels. In order to find the genes connected with bioaccumulation of metals, the root transcriptome annotation of Sonneratia alba was analyzed and a new metallothionein (MT) gene was cloned. Sequence analysis found that the new MT gene belongs to type 3 MT, which is mostly expressed in roots. A simple and efficient method was used to express the type 3 MT of S. alba (SaMT3) by transforming the recombinant expression vector pET15b-SaMT3 into Escherichia coli (E. coli) Rosetta-gami and induction with the optimal conditions of 500 μM Isopropyl β-D-1-thiogalactopyranoside (IPTG) at 24ºC for 12 h. OD 600

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“…Glutathione (GSH) serves as the substrate of PC n biosynthesis through the transpeptidation of the γ-Glu-Cys moiety of GSH onto a second GSH to form PC 2 or onto a PC n molecule to produce an n+1 oligomer (Stillman et al, 1992;Bordin, 2000;Cobbett, 2000;Cobbett and Goldsbrough, 2002; Riordan and Vallee, 1991;Suzuki et al, 1993). By other hand, PC n synthesis depends not only on the plant species but also of the metal stressor, being Cd the best activator followed by Ag, Bi, Pb, Zn, Cu, Hg and Au cations (Bundy et al 2014;Dago 2014b). This relationship of PC n with trace metal contamination allows us to use PC n content as an indicator of metal pollution.…”

Section: Introductionmentioning

confidence: 99%

Phytochelatin synthesis in response to Hg uptake in aquatic plants near a chlor-alkali factory

et al. 2017

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Chemometrics applied to the analysis of induced phytochelatins in Hordeum vulgare plants stressed with various toxic non-essential metals and metalloids

Cited by 29 publications

References 22 publications

Evaluation of Mercury Stress in Plants from the Almadén Mining District by Analysis of Phytochelatins and Their Hg Complexes

Evaluation of Mercury Stress in Plants from the Almadén Mining District by Analysis of Phytochelatins and Their Hg Complexes

Gene Cloning, Protein Expression and Functional Analysis of a type 3 Metallothionein with Bioaccumulation Potential from Sonneratia alba

Phytochelatin synthesis in response to Hg uptake in aquatic plants near a chlor-alkali factory

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