Chemometric-assisted method development in hydrophilic interaction liquid chromatography: A review

Recent Advances in Chromatography for Pharmaceutical Analysis

“…A recent review summarized the possibilities of chemometric-assisted method development in HILIC. 195 Some recent works focused on tryptic peptide retention modeling in HILIC for proteomic purposes. 117,196,197 Those models can help with peptide identification from protein digests and decrease the risk of false positive results.…”

Introductory Chapter: Mathematical Methods in Liquid Chromatography - The State-of-the-Art Developments and Challenges

“…Hydrophilic interaction liquid chromatography (HILIC) is an alternative HPLC mode that utilizes hydro-organic mobile phases with a high organic content and a hydrophilic stationary phase for separating predominantly polar compounds and charges substances in complex matrices. With an enormous growth in the application of HILIC, computer-aided modeling (most importantly the QSRR modeling strategy) proved to be useful in understanding the retention mechanism, classification of stationary phases, prediction of retention times, optimization of chromatographic conditions, and interaction effects of chromatographic factors [25].…”

Modulated Preparation of Capillary Monolithic HILIC Column by Target‐Analogues of Matrine and Oxymatrine and Applied for Extracted Analysis of Sophorae flavescentis radix

“…Hydrophilic interaction liquid chromatography (HILIC), as an important alternative of reverse‐phase liquid chromatography (RPLC) and a facile LC method equipped with a mass spectrometry (MS) compatible solvent system, has gained increasing attention and seen much progress especially in the design of new HILIC stationary phases and the development of HILIC analytical methods . The HILIC stationary phases, synthesized by monomers with particular functional groups and forming with desired pore size distribution, are usually tested by a group of standardized substance representing different molecular force types.…”

“…The HILIC stationary phases, synthesized by monomers with particular functional groups and forming with desired pore size distribution, are usually tested by a group of standardized substance representing different molecular force types. The HILIC analytical methods, are usually established by systematic optimization methods such as AQbD or chemometrics to explore important experimental factors on one fixed HILIC column for complicated sample. However, the regulation of stationary phase and running conditions is ultimately aimed at the analysis of real sample to obtain information about its specific components or non‐targeted holistic chromatographical information.…”