Chemokines as Potential Markers in Pediatric Renal Diseases

Silva, Ana Cristina Simões e; Pereira, André; Teixeira, Mauro Martins; Teixeira, Antônio Lúcio

doi:10.1007/978-94-007-7699-9_39

Biomarkers in Kidney Disease 2016

DOI: 10.1007/978-94-007-7699-9_39

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Chemokines as Potential Markers in Pediatric Renal Diseases

Ana Cristina Simões e Silva¹,

André Pereira²,

Mauro Martins Teixeira³

et al.

Abstract: Glomerular diseases and obstructive uropathies are the two most frequent causes of chronic kidney disease (CKD) in children. Recently, biomarkers have become a focus of clinical research as potentially useful diagnostic tools in pediatric renal diseases. Among several putative biomarkers, chemokines emerge as promising molecules since they play relevant roles in the pathophysiology of pediatric renal diseases. The evaluation of these inflammatory mediators might help in the management of diverse renal diseases… Show more

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2022

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Distance-Based All-In-One Immunodevice for Point-of-Care Monitoring of Cytokine Interleukin-6

2022

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We report the development of a distance-based paper analytical device combined with a hydrophilic bridge valve (B-dPAD) as a quantitative immunoassay method to monitor human interleukin-6 (IL-6) in human samples. Our device design features (i) a circular sample inlet zone, (ii) a circular capture zone with immobilized anti-IL-6 (anti-Ab 1 ), and (iii) a detection zone channel coated with methylene blue (MB). Two hydrophilic valves are positioned between these three zones. IL-6 levels were determined quantitatively by measuring the extent of degradation of MB to a colorless product along the length of the detection zone channel. Following method optimization, we obtained a linear range from 0.05 to 25.0 pg/mL (R 2 = 0.9995) and a detection limit (LOD) of 0.05 pg/mL by the naked-eye readout. This is directly within the clinically relevant range. The system does not require any external instrumentation, and the bridge valves can be easily connected and disconnected by a minimally trained operator. The total analysis time is 35 min, significantly reduced from a typical ELISA assay, which takes around 1 h since the B-dPAD workflow circumvents washing steps. The device was tested for IL-6 quantification in human saliva and urine samples of volunteers, with no significant difference found between our method and the standard clinical laboratory method at 95% confidence levels. Recoveries ranged from 98 to 105% with the highest standard deviation at 3.9%. Our B-dPAD immunodevice is therefore a promising approach for rapid IL-6 monitoring in the context of point-of-care diagnostics and analysis in resource-limited settings.

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Distance-Based All-In-One Immunodevice for Point-of-Care Monitoring of Cytokine Interleukin-6

2022

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Chemokines as Potential Markers in Pediatric Renal Diseases

Cited by 1 publication

References 78 publications

Distance-Based All-In-One Immunodevice for Point-of-Care Monitoring of Cytokine Interleukin-6

Distance-Based All-In-One Immunodevice for Point-of-Care Monitoring of Cytokine Interleukin-6

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