Chemokines and ischemic stroke

Peters, Elaine E.; Feuerstein, Giora

doi:10.1007/978-3-0348-8297-2_12

Cited by 1 publication

(1 citation statement)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Increasingly, naturally‐occurring or synthetic chemical compounds shown to have antioxidant and/or free radical scavenging activity, have provided the most promise in protecting against neurodegenerative and neuro‐inflammatory diseases . By scavenging free radicals that are produced during neurodegeneration, antioxidants have been shown to provide neuroprotection against the cell death associated with ischaemia‐reperfusion injury …”

Section: Introductionmentioning

confidence: 99%

A novel synthetic chemical entity (UPEI‐800) is neuroprotective in vitro and in an in vivo rat model of oxidative stress

Saleh

Connell

et al. 2017

Clin Exp Pharma Physio

View full text Add to dashboard Cite

In this study, we tested a novel synthetic pyrazole-containing compound, 5-amino-1-phenyl-1H-pyrazole-4-carbonitrile (APPC), as an antioxidant in both in vitro and in vivo models of oxidative stress. In addition, the utility of covalently combining APPC with another well-established antioxidant, lipoic acid (LA), was also tested in both models. The in vitro results demonstrated that pretreatment with APPC in a mixed neuronal-glial culture exposed to oxygen-glucose deprivation (OGD) followed by reoxygenation-refeeding, resulted in significant neuroprotection at concentrations between 2.5 to 25 μmol/L. In contrast, LA was not neuroprotective following OGD alone or following reoxygenation-refeeding. However, the synthetic covalent combination of APPC with LA, named "UPEI-800", resulted in significant neuroprotection at concentrations between 0.027 and 2.7 μmol/L (100-fold more potent than APPC alone), an effect shown to be correlated with increased cellular antioxidant capacity. Further, in an in vivo model of ischaemia-reperfusion injury following transient occlusion of the middle cerebral artery (tMCAO), both APPC (0.1 and 1.0 mg/kg) and UPEI-800 (1×10 mg/kg) provided significant neuroprotection. Consistent with the in vitro findings, the in vivo results following tMCAO also demonstrated a 100-fold increase in the potency of the covalently linked compound UPEI-800 compared to APPC alone.

show abstract

Section: Introductionmentioning

confidence: 99%