Chemokine Receptors CCR1 and CCR2 on Peripheral Blood Mononuclear Cells of Newly Diagnosed Patients with the CD38-Positive Chronic Lymphocytic Leukemia

Abstract

Chemokines and their receptors direct migration and infiltration of immune cells. CCR1 and CCR2 maintain sequence similarity and respond to a number of the same chemokines secreted in lymphoid organs. Expression of CD38 on leukemic cells has been associated with poor clinical outcomes in patients with chronic lymphocytic leukemia (CLL) and is considered as the negative predictor of progression. In our study of newly diagnosed CLL patients, which included 39 CD38-positive and 22 CD38-negative patients, CCR1 and/or CCR2 were always detected, using flow cytometry, on the peripheral blood (PB) CD19+CD5+ lymphocytes in patients with >30% of the CD38+ CD19+CD5+ lymphocytes (n = 16). Spearman’s rank correlation analysis determined correlations between the frequency of the CCR1- and CCR2-expressing PB CD19+CD5+ lymphocytes and the frequency of the CD38-positive CD19+CD5+ lymphocytes (rs = 0.50 and rs = 0.38, respectively). No significant correlations were observed between ZAP70 mRNA expression levels in PB mononuclear cells and the frequency of the circulating CCR1+ or CCR2+ CD19+CD5+ lymphocytes. Further association studies are needed to verify prognostic relevance of the CCR1/CCR2 expression on leukemic cells in CLL patients at diagnosis. We suggest that CCR1/CCR2 signaling pathways could represent attractive targets for development of CLL anti-progression therapeutics.

Expression of the Chemokine Receptor CCR1 in Burkitt Lymphoma Cell Lines Is Linked to the CD10-Negative Cell Phenotype and Co-Expression of the EBV Latent Genes EBNA2, LMP1, and LMP2

“…We did not detect CCR2 on the cell surface of the LCL cells nor BL cells after serum deprivation. Nevertheless, using the same mouse monoclonal anti-CD192-Alexa Fluor 647 antibodies, we demonstrated the cell-surface expression of CCR2 on monocytes and B lymphocytes in PB of patients with rheumatoid arthritis [ 24 ] and patients with chronic lymphocytic leukemia [ 25 ].…”

Lymphoma: New Diagnosis and Current Treatment Strategies

“…Finally, the paper by Kholodnyk et al focuses on the role of chemokine receptors CCR1 and CCR2 on mononuclear cells in CD38-positive CLL [ 10 ]. CCR1 and CCR2 may play a role in the development of inflammatory disorders, AIDs, and cancer progression [ 11 ].…”