Chemokine receptor’s expression in human adipose derived mesenchymal stem cells primed with valproic acid

Hashemzadeh, Mohammad Reza; Seyedi, Zahra; Rafiei, Samaneh; Hassanzadeh-Moghaddam, Maryam; Edalatmanesh, Mohammad Amin

doi:10.1007/s00580-016-2352-8

Cited by 6 publications

(3 citation statements)

References 23 publications

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“…Moreover, in a study, it was demonstrated that pre-treatment with VPA before transplantation enhances the therapeutic benefits of bone marrow MSCs (BMSCs) in terms of greater cell migration and better neurological outcomes through increasing the expression of CXCR4 after traumatic acute spinal cord injury (SCI) in animal models (Chen et al 2014). Also, it was demonstrated that VPA treatment enhances homing of Ad-MSCs via overexpression of CXCR4 and CXCR6 chemokine receptors (Hashemzadeh et al 2017), as it was shown in another study that pre-treating CMSC29 placenta-derived mesenchymal stem/stromal cell line with VPA could and CoCl 2 only upregulated VEGF-A and VEGF-C gene expression (d, e). The expression of MMP9 was decreased after pre-treatments of the cells with VPA and CoCl 2 (c).…”

Section: Discussionmentioning

confidence: 99%

Comparison the effects of hypoxia-mimicking agents on migration-related signaling pathways in mesenchymal stem cells

et al. 2020

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Adipose-derived mesenchymal stem cells (Ad-MSCs) have been designated as the promising agents for clinical applications for easy accessibility, multi-linage differentiation and immunomodulation capacity. Despite this, optimal cell delivery conditions have remained as a clinical challenge and improvement of stem cell homing to the target organs is being considered as a major strategy in cell therapy systemic injection. It has been shown that homing of mesenchymal stem cells are increased when treated with physical or chemical hypoxia-mimicking factors, however, efficiency of different agents remained to be determined. In this study, hypoxia-mimicking agents, including valproic acid (VPA), cobalt chloride (CoCl 2) and deferoxamine (DFX) were examined to determine whether they are able to activate signaling molecules involved in migration of Ad-MSCs in vitro. We report that Ad-MSCs treated by DFX resulted in a significantly enhanced mRNA expression of MAPK4 (associated with MAPK signaling pathway), INPP4B (associated with Inositol polyphosphate pathway), VEGF-A and VEGF-C (associated with cytokinecytokine receptor pathways), IL-8 and its receptor, CXCR2 (associated with IL-8 signaling pathway). While the cells treated with VPA did not show such effects and CoCl 2 only upregulated VEGF-A and VEGF-C gene expression. Furthermore, results of wound-healing assays showed migration capacity of Ad-MSCs treated with DFX significantly increased 8 and 24 h of the treatment. This study provides credible evidence around DFX, which might be an effective drug for pharmacological preconditioning of Ad-MSCs to boost their homing capacity and regeneration of damaged tissues though, activation of the migration-related signaling pathways.

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Section: Discussionmentioning

confidence: 99%

Comparison the effects of hypoxia-mimicking agents on migration-related signaling pathways in mesenchymal stem cells

et al. 2020

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“…Valproic acid demonstrated a wide range of neuroprotective properties in cellular and animal models of neurodegenerative diseases[ 71 , 72 ], probably for the activity as both toward the inhibition of glycogen synthase kinase-3 (GSK-3)[ 73 ] and the enhancement of CXCR4 expression[ 74 ]. In ASCs, in vitro treatment with valproic acid resulted in a promotion of neuron-like differentiation[ 75 ] and in vivo an enhanced homing of ASCs was reported via overexpression of CXCR4 and CXCR6[ 76 ]. Indeed, the demethylating agent 5-azacytidine is commonly employed to treat blood disorders such as myelodysplasia and leukemia[ 77 ].…”

Section: Growth Factors and Chemicals For Ascs Neural Differentiationmentioning

confidence: 99%

Physiologically based microenvironment forin vitroneural differentiation of adipose-derived stem cells

Graziano

Avola

Perciavalle

et al. 2018

WJSC

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The limited capacity of nervous system to promote a spontaneous regeneration and the high rate of neurodegenerative diseases appearance are keys factors that stimulate researches both for defining the molecular mechanisms of pathophysiology and for evaluating putative strategies to induce neural tissue regeneration. In this latter aspect, the application of stem cells seems to be a promising approach, even if the control of their differentiation and the maintaining of a safe state of proliferation should be troubled. Here, we focus on adipose tissue-derived stem cells and we seek out the recent advances on the promotion of their neural differentiation, performing a critical integration of the basic biology and physiology of adipose tissue-derived stem cells with the functional modifications that the biophysical, biomechanical and biochemical microenvironment induces to cell phenotype. The pre-clinical studies showed that the neural differentiation by cell stimulation with growth factors benefits from the integration with biomaterials and biophysical interaction like microgravity. All these elements have been reported as furnisher of microenvironments with desirable biological, physical and mechanical properties. A critical review of current knowledge is here proposed, underscoring that a real advance toward a stable, safe and controllable adipose stem cells clinical application will derive from a synergic multidisciplinary approach that involves material engineer, basic cell biology, cell and tissue physiology.

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“…Recent experiments on animal model of stroke showed that VPA causes multiple beneficial effects including induction of neurogenesis, suppression of neuroinflammation and infarct volume reduction [10,21]. Additionally VPA acts as a histone deacetylas inhibitor that have effective role in transcriptional regulation and can increase the expression of CXCR4 in stem cells [22,23]. In this study, VPA was used to treat NSCs in order to investigate its effects on expression of some important chemokine receptors in homing such as CXCR4, CXCR6, CCR1 and CCR7.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of Chemokine Receptors on Neural Stem Cells Pretreated with Valproic acid: Towards Improved Homing

Karimi¹,

Hashemzadeh²,

Irfan-Maqsood³

et al. 2016

CTRM

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Neural stem cells (NSCs) have considerable capacity for self-renewing and also ability for generating neurons in the mammalian brain. However, one of the big challenges is the migration and targeted homing of transplanted NSCs into the injured site to treat neurodegenerative diseases including Alzheimer´s disease (AD), Parkinson's disease (PD), multiple sclerosis (MS), amyotrophic lateral sclerosis (ALS), brain ischemia (BI) and spinal cord injury (SCI). To improve homing capacity, pretreatment of NSCs with Valproic acid (VPA), which is supposed to cause diverse effects on migration ability of NSCs, is a strategy. More recently, hind brain and olfactory bulbs have been introduced as a good source of NSCs. So, NSCs were isolated from these two sources of postnatal day 1 (PND1) rats. These isolated cells were characterized by expressing neuronal markers such as Nestin and Sox2. The expression of four selected chemokine receptors (CXCR4, CXCR6, CCR1 and CCR7), which are important effectors in homing of stem cells, was investigated. It is concluded that VPA treatment enhances NSCs migration and homing showing its potential to be applied for cell-based therapies.

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Chemokine receptor’s expression in human adipose derived mesenchymal stem cells primed with valproic acid

Cited by 6 publications

References 23 publications

Comparison the effects of hypoxia-mimicking agents on migration-related signaling pathways in mesenchymal stem cells

Comparison the effects of hypoxia-mimicking agents on migration-related signaling pathways in mesenchymal stem cells

Physiologically based microenvironment forin vitroneural differentiation of adipose-derived stem cells

Overexpression of Chemokine Receptors on Neural Stem Cells Pretreated with Valproic acid: Towards Improved Homing

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