Chemokine-Induced Macrophage Polarization in Inflammatory Conditions

Ruytinx, Pieter; Proost, Paul; Damme, Jozef Van; Struyf, Sofie

doi:10.3389/fimmu.2018.01930

Cited by 309 publications

(252 citation statements)

References 168 publications

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“…However, this classification is useful in providing an overview of the diverging cellular responses to CCL2. Multiple cytokines have been described in the complex network of macrophage polarization, and their roles have been successively revealed (151,152). The M1 phenotype is characterized by increased expression of cytokines, such as TNFα, IL-1β, IL-6, and IL-12; increased expression of surface receptors, such as Fcy-RI, II, and IIIA; as well as the production of reactive oxygen species (ROS) and reactive nitrogen species (151,153).…”

Section: Ccl2 Induces Context-specific Macrophage Polarization and Cymentioning

confidence: 99%

More Than Just Attractive: How CCL2 Influences Myeloid Cell Behavior Beyond Chemotaxis

2019

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Monocyte chemoattractant protein-1 (MCP-1/CCL2) is renowned for its ability to drive the chemotaxis of myeloid and lymphoid cells. It orchestrates the migration of these cell types both during physiological immune defense and in pathological circumstances, such as autoimmune diseases including rheumatoid arthritis and multiple sclerosis, inflammatory diseases including atherosclerosis, as well as infectious diseases, obesity, diabetes, and various types of cancer. However, new data suggest that the scope of CCL2's functions may extend beyond its original characterization as a chemoattractant. Emerging evidence shows that it can impact leukocyte behavior, influencing adhesion, polarization, effector molecule secretion, autophagy, killing, and survival. The direction of these CCL2-induced responses is context dependent and, in some cases, synergistic with other inflammatory stimuli. The involvement of CCL2 signaling in multiple diseases renders it an interesting therapeutic target, although current targeting strategies have not met early expectations in the clinic. A better understanding of how CCL2 affects immune cells will be pivotal to the improvement of existing therapeutic approaches and the development of new drugs. Here, we provide an overview of the pleiotropic effects of CCL2 signaling on cells of the myeloid lineage, beyond chemotaxis, and highlight how these actions might help to shape immune cell behavior and tumor immunity.

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Section: Ccl2 Induces Context-specific Macrophage Polarization and Cymentioning

confidence: 99%

More Than Just Attractive: How CCL2 Influences Myeloid Cell Behavior Beyond Chemotaxis

2019

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“…Excessive production of proinflammatory mediators from these macrophages might have effects on the parasites, but could also lead to excessive injury of surrounding tissue and hence result in extensive periductal fibrosis. As alternatively activated (M2) macrophages are known to play an important role in wound healing and acquire a pro-fibrotic phenotype (27), our MDM activities reported here may be predominantly generated by M2 macrophages. More in-depth mechanisms on macrophage sub-type responses are required.…”

Section: Discussionmentioning

confidence: 82%

High macrophage activities are associated with advanced periductal fibrosis in chronic Opisthorchis viverrini infection

Salao

Watakulsin

Mairiang

et al. 2018

Parasite Immunology

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Liver fluke infection caused by Opisthorchis viverrini induces several hepatobiliary conditions including advanced periductal fibrosis (APF) and cholangiocarcinoma (CCA), but >25% of the infected population develops APF and 1% develop CCA. The innate immune response is the first line of defense, and macrophages are critical regulators of fibrosis. We hypothesized that macrophages from infected individuals have different capacities to either promote or suppress periductal fibrosis. We compared phagocytic activities of macrophages of healthy individuals and O. viverrini-infected individuals ±APF, and found that macrophages from infected individuals with APF ingested significantly higher numbers of beads compared with healthy controls and O. viverrini-infected individuals without APF. To further investigate proteolytic activity, we monitored real-time phagosomal proteolysis of beads conjugated to DQ BODIPY-BSA using live-cell imaging. We show that macrophages from O. viverrini-infected individuals with APF also have elevated phagosomal proteolysis activity, which is consistent with their increased phagocytic activity. Additionally, stimulated ROS production by blood monocytes was higher in individuals with APF compared with healthy controls and infected individuals without APF. These results suggest that during O. viverrini infection, macrophages with high phagocytic and proteolytic activities together with elevated ROS production, are the phenotypes that can promote tissue damage, which results in periductal fibrosis.

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“…Actin cytoskeleton and cell adhere, are closely related to epithelial to mesenchymal transition (EMT) and cell migration (39,40). Chemokine can recruit macrophage and induced macrophage M2 polarization which promoted tumor lymphatic metastasis (41). MAPK and TGF-beta signaling pathway has been known to mediate cell EMT and migration in thyroid carcinomas (42,43).…”

Section: Discussionmentioning

confidence: 99%

OTUD6B-AS1 Inhibits Viability, Migration, and Invasion of Thyroid Carcinoma by Targeting miR-183-5p and miR-21

et al. 2020

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Background: The long noncoding RNA (lncRNA) functions as a regulator of initiation, progression, and metastasis of thyroid carcinomas. lncRNA OTUD6B antisense RNA 1 (OTUD6B-AS1) is a tumor-suppressive noncoding RNA in clear cell renal cell carcinoma. The role of OTUD6B-AS1 in thyroid carcinomas has not been reported yet. We aim to investigate the expression and biological functions of OTUD6B-AS1 in thyroid carcinomas. Methods:The expression level of OTUD6B-AS1 was measured in 60 paired human thyroid carcinoma tissues and corresponding adjacent normal thyroid tissues. The correlations between the OTUD6B-AS1 expression levels and clinicopathological features were evaluated using the Mann-Whitney test. The effects of OTUD6B-AS1 on thyroid carcinoma cells were determined via the MTT and transwell assays. The potential targets of OTUD6B-AS1 were screened using the online programs OncomiR and StarBase 3.0, and the LncBase Predicted v.2. Luciferase reporter assay was used to confirm the interactions between OTUD6B-AS1 and its potential targets.Results: OTUD6B-AS1 was downregulated in thyroid carcinoma tissue samples. The expression of OTUD6B-AS1 correlated with tumor size, clinical stage, and lymphatic metastasis of thyroid carcinoma. Overexpression of OTUD6B-AS1 significantly decreased the viability, migration, and invasion of thyroid carcinoma cells. Online programs predicted miR-183-5p and miR-21 as potential targets of OTUD6B-AS1. Luciferase reporter assays showed miR-183-5p and miR-21 bound to OTUD6B-AS1. Moreover, overexpression of miR-183-5p and miR-21 compromised the inhibitory effects of OTUD6B-AS1 on viability, migration, and invasion of thyroid carcinoma cells.Conclusions: Taken together, our findings present in vitro evidence of lncRNA OTUD6B-AS1 as a tumor suppressor in thyroid carcinomas. OTUD6B-AS1 inhibits viability, migration, and invasion of thyroid carcinoma by targeting miR-183-5p and miR-21.

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Chemokine-Induced Macrophage Polarization in Inflammatory Conditions

Cited by 309 publications

References 168 publications

More Than Just Attractive: How CCL2 Influences Myeloid Cell Behavior Beyond Chemotaxis

More Than Just Attractive: How CCL2 Influences Myeloid Cell Behavior Beyond Chemotaxis

High macrophage activities are associated with advanced periductal fibrosis in chronic Opisthorchis viverrini infection

OTUD6B-AS1 Inhibits Viability, Migration, and Invasion of Thyroid Carcinoma by Targeting miR-183-5p and miR-21

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