Chemogenetic manipulation of astrocytic signaling in the basolateral amygdala reduces binge‐like alcohol consumption in male mice

Nwachukwu, Kala N.; Evans, William Andrew; Sides, Tori; Trevisani, C; Davis, Ambryia; Marshall, S. Alex

doi:10.1002/jnr.24841

Cited by 23 publications

(7 citation statements)

References 112 publications

(150 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Research showed that in vivo activation of Gq‐DREADDs, expressed in astrocytes of the nucleus accumbens core, modulated activity of the surrounding neuropil via release of glutamate (Scofield et al, 2015). Furthermore, glutamate concentrations were elevated in the amygdala after activation of Gq‐DREADDs expressed on astrocytes, which in turn reversed the reduced glutamate levels after ethanol consumption in mice (Nwachukwu et al, 2021). Likewise, activation of Gq‐DREADDs expressed in astrocytes of hippocampal and striatal brain slices was proposed to elicit glutamate release (Chai et al, 2017; Durkee et al, 2019).…”

Section: Application Of Dreadds In Astrocytesmentioning

confidence: 99%

Chemogenetic modulation of astrocytes and microglia: State‐of‐the‐art and implications in neuroscience

Bossuyt

Herrewegen

Nestor

et al. 2023

Glia

View full text Add to dashboard Cite

Insights into the role astrocytes and microglia play in normal and diseased brain functioning has expanded drastically over the last decade. Recently, chemogenetic tools have emerged as cutting-edge techniques, allowing targeted and spatiotemporal precise manipulation of a specific glial cell type. As a result, significant advances in astrocyte and microglial cell function have been made, showing how glial cells can intervene in central nervous system (CNS) functions such as cognition, reward and feeding behavior in addition to their established contribution in brain diseases, pain, and CNS inflammation. Here, we discuss the latest insights in glial functions in health and disease that have been made through the application of chemogenetics. We will focus on the manipulation of intracellular signaling pathways induced by activation of the designer receptors exclusively activated by designer drugs (DREADDs) in astrocytes and microglia. We will also elaborate on some of the potential pitfalls and the translational potential of the DREADD technology.

show abstract

Section: Application Of Dreadds In Astrocytesmentioning

confidence: 99%

Chemogenetic modulation of astrocytes and microglia: State‐of‐the‐art and implications in neuroscience

Bossuyt

Herrewegen

Nestor

et al. 2023

Glia

View full text Add to dashboard Cite

show abstract

“…Esta técnica se utiliza para modificar la actividad astrocitaria que en general resulta en cambios en la señalización del Ca 2+ y diversos efectos en otras células cerebrales (45,46). La principal ventaja de esta técnica es la activación o inhibición específica de los astrocitos frente al enfoque farmacológico clásico que afecta de manera menos específica pudiendo alterar la función neuronal y/o producir diferentes efectos secundarios (47)(48)(49)(50).…”

Section: Modelos Experimentales De Epileptogénesisunclassified

Experimental models in the study of epileptogenesis

et al. 2022

View full text Add to dashboard Cite

Epilepsy is a chronic neurological disease characterized by spontaneous recurrently occurring epileptic seizures as a consequence of abnormal, excessive and synchronous neuronal activity in the brain. Temporal lobe epilepsy (TLE) is the most common form of focal epilepsy in adults, being characterized by hippocampal sclerosis, reactive gliosis, neurodegeneration, and synaptic reorganization. Animal models of TLE based on the administration of convulsive agents trigger a status epilepticus (SE) that progresses towards the occurrence of spontaneous recurrent seizures. Among these models are those induced by the systemic administration of pilocarpine or by intrahippocampal injection of kainic acid, both being characterized by 3 clearly defined phases: (i) acute SE seizures; (ii) latent period and (iii) occurrence of recurrent spontaneous seizures. These models not only reproduce most of the neuropathological TLE features but also allow for the identification of biomarkers of epileptogenesis and potential pharmacological targets. The use of neuroimaging techniques such as positron emission tomography (PET) with the radiotracer 18F-Fluorodeoxyglucose (18F-FDG) identifies brain hypometabolism in the latent period that not only localizes the epileptic focus but is also an biomarker of early diagnosis. Other neuroimaging techniques allow for detecting, among others, biomarkers of neuroinflammation, alterations in the permeability of the blood-brain barrier and astrocytic activation, all of them associated with epileptogenesis. Finally, the use of chemogenetics through DREADDs (Designer Receptors Exclusively Activated by Designer Drugs) technology in murine models leads to targeted modulation of astrocytic activity, being a novel tool that considers the contribution of the astrocytes role in brain metabolic alterations in epileptogenesis.

show abstract

“…Activation of PFC astrocytes increased ethanol consumption in ethanol-naïve male mice whereas reduction of astrocyte calcium via CalEx expression reduced ethanol drinking indicating a bidirectional regulation of ethanol-consumption by PFC astrocytes ( Erickson et al, 2021 ). In the basolateral amygdala, activation of astrocytes reduced ethanol consumption in male mice ( Nwachukwu et al, 2021 ). Likewise, in the nucleus accumbens (NAc), a key reward center, activation of astrocytes in male rats decreased ethanol-seeking behavior after abstinence ( Bull et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

From Synapses to Circuits, Astrocytes Regulate Behavior

Lyon

Allen

2022

Front. Neural Circuits

View full text Add to dashboard Cite

Astrocytes are non-neuronal cells that regulate synapses, neuronal circuits, and behavior. Astrocytes ensheath neuronal synapses to form the tripartite synapse where astrocytes influence synapse formation, function, and plasticity. Beyond the synapse, recent research has revealed that astrocyte influences on the nervous system extend to the modulation of neuronal circuitry and behavior. Here we review recent findings on the active role of astrocytes in behavioral modulation with a focus on in vivo studies, primarily in mice. Using tools to acutely manipulate astrocytes, such as optogenetics or chemogenetics, studies reviewed here have demonstrated a causal role for astrocytes in sleep, memory, sensorimotor behaviors, feeding, fear, anxiety, and cognitive processes like attention and behavioral flexibility. Current tools and future directions for astrocyte-specific manipulation, including methods for probing astrocyte heterogeneity, are discussed. Understanding the contribution of astrocytes to neuronal circuit activity and organismal behavior will be critical toward understanding how nervous system function gives rise to behavior.

show abstract

Chemogenetic manipulation of astrocytic signaling in the basolateral amygdala reduces binge‐like alcohol consumption in male mice

Cited by 23 publications

References 112 publications

Chemogenetic modulation of astrocytes and microglia: State‐of‐the‐art and implications in neuroscience

Chemogenetic modulation of astrocytes and microglia: State‐of‐the‐art and implications in neuroscience

Experimental models in the study of epileptogenesis

From Synapses to Circuits, Astrocytes Regulate Behavior

Contact Info

Product

Resources

About