D.V. Gokhale, K.B. Bastawde, S.G. Patil, U.R. Kalkote, R.R. Joshi, R.A. Joshi, T. Ravindranathan, B.G. Gaikwad, V.V. Jogdand, S. Nene
Abstract:We screened 125 Pseudomonas strains from our culture collection for the production of hydantoinase activity using DL-phenylhydantoin as a substrate. Pseudomonas desmolyticum NCIM 2112 was found to be the best hydantoinase (dihydropyrimidinase E.C. 18.104.22.168) producer. The enzymatic reactions were carried out using 18-20-h grown cells in nutrient broth and 5-phenylhydantoin as the substrate. Optimization studies for the biotransformation reaction were performed to increase product yield. The optimum pH and temper…
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