Chemoenzymatic preparation of (S)-p-nitrostyrene oxide from p-nitrophenacyl bromide by recombinant Escherichia coli cells expressing a novel halohydrin dehalogenase

Biosynthesis of Phenylglyoxylic Acid by LhDMDH, a Novel d-Mandelate Dehydrogenase with High Catalytic Activity

“…Unfortunately, the residual activity of LhDMDH was less than 50% after being incubated at 30 °C for 1 h, and its low thermostability would be impeded during biosynthesis of PGA. Hence, whole cell catalysis is a preferred option to eliminate the influence of low thermostability in the biosynthesis of PGA. , It should be noted that with the emergence of various bioinformatics software, in silico design is playing a more significant role in molecular modification of thermostability. In particular, molecular dynamics simulation has become a more mature tool .…”

Halohydrin dehalogenase-catalysed synthesis of enantiopure fluorinated building blocks: bottlenecks found and explained by applying a reaction engineering approach

“…Moreover, whole-cell biotransformations with HHDHs are often encountered in the literature. 49–53 In order to increase the reaction productivity, non-conventional media should be considered. Future research should focus on HHDH compatibility with alternative media in order to reduce the hydrolysis impact and to minimize the inhibition by distribution of enzymes and inhibitors in different phases.…”

Low-temperature N-anchored ordered Pt3Co intermetallic nanoparticles as electrocatalysts for methanol oxidation reaction

“…Due to the electron-donating effect of N, the introduction of N into CNTs increases the electronic conductivity of CNTs, and N-CNTs are more conducive to the immobilization of Pt 3 Co NPs via the enhanced metal–support interactions. 56…”