Chemoenzymatic Dynamic Kinetic Resolution of Primary Benzylic Amines using Pd⁰‐CalB CLEA as a Biohybrid Catalyst

Abstract

Herein, we report on the use a biohybrid catalyst consisting of palladium nanoparticles immobilized on cross‐linked enzyme aggregates of lipase B of Candida antarctica (CalB CLEA) for the dynamic kinetic resolution (DKR) of benzylic amines. A set of amines were demonstrated to undergo an efficient DKR and the recyclability of the catalysts was studied. Extensive efforts to further elucidate the structure of the catalyst are presented.

Optimisation of catalysts coupling in multi-catalytic hybrid materials: perspectives for the next revolution in catalysis

“…Nevertheless, the high stability of CLEAs and their ease of synthesis make this enzyme immobilisation method very popular. CLEAs have been used for the synthesis of MCHMs, as described by Bäckvall et al 21,22 Finally, it is even possible to directly use metals for CLEA formation, e.g., Li et al used copper…”

Efficient production of wild-type lipase B from Candida antarctica in the cytoplasm of Escherichia coli

“…It remains stable from pH 3.5 -9.5 (optimal catalysis at pH 7) and has been observed to function at 150°C [4], in polar organic solvents [5], within ionic liquids and supercritical carbon dioxide [4]. The enzyme is an efficient biocatalyst and can be utilized in a broad range of processes, from biodiesel production (reviewed in [6][7][8][9][10][11][12]), glycerol carbonate [13], diacylglycerol [14][15], benzyl acetate [16], olvanil [17] and drug synthesis, [18] racemate resolutions [19][20][21][22][23], lignin valorization [24] and aminolysis [25].…”

Developing Multicompartment Biopolymer Hydrogel Beads for Tandem Chemoenzymatic One-Pot Process

“…Therefore, the development of new catalytic processes featuring both chemo-and biocatalysis has increasingly been reported in recent years [9][10][11][12][13][14]. Those reports feature the combination of enzymes with metalor organocatalysts, with reaction sequences conducted either in a classical sequential way or in tandem [15][16][17][18][19][20][21][22][23][24][25][26].…”