Chemoautotroph Cupriavidus necator as a potential game-changer for global warming and plastic waste problem: A review

Sohn, Yu Jung; Son, Jina; Jo, Seo Young; Park, Se Young; Yoo, Jee In; Baritugo, Kei-Anne; Na, Jeong-Geol; Choi, Jong‐il; Kim, Hee Taek; Joo, Jeong Chan; Park, Si Jae

doi:10.1016/j.biortech.2021.125693

Cited by 65 publications

(22 citation statements)

References 137 publications

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“…The two‐stage bioprocess developed in Cestellos‐Blanco et al ( 2021 ) showed a peak of acetate production of 10.4 mmol acetate L −1 d −1 from CO 2 by Sporumosa ovata , which subsequently translated into 12.54 mg PHB L −1 h −1 by Cupriavidus basilensis in the unprocessed media with an overall carbon yield of 11.06% from acetate. In this case, the production of PHB from CO 2 occurred with limited intermediate purification and processing steps but can expectedly improve by undertaking further optimizations of each biocatalyst step (Sohn et al, 2021 ). According to the data reported in ref.…”

Section: Biological Two‐stage Processes For Products' Synthesis From ...mentioning

confidence: 99%

Leveraging substrate flexibility and product selectivity of acetogens in two‐stage systems for chemical production

Ricci

Seifert²,

Bernacchi³

et al. 2022

Microbial Biotechnology

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Carbon dioxide (CO 2 ) stands out as sustainable feedstock for developing a circular carbon economy whose energy supply could be obtained by boosting the production of clean hydrogen from renewable electricity. H 2 ‐dependent CO 2 gas fermentation using acetogenic microorganisms offers a viable solution of increasingly demonstrated value. While gas fermentation advances to achieve commercial process scalability, which is currently limited to a few products such as acetate and ethanol, it is worth taking the best of the current state‐of‐the‐art technology by its integration within innovative bioconversion schemes. This review presents multiple scenarios where gas fermentation by acetogens integrate into double‐stage biotechnological production processes that use CO 2 as sole carbon feedstock and H 2 as energy carrier for products' synthesis. In the integration schemes here reviewed, the first stage can be biotic or abiotic while the second stage is biotic. When the first stage is biotic, acetogens act as a biological platform to generate chemical intermediates such as acetate, formate and ethanol that become substrates for a second fermentation stage. This approach holds the potential to enhance process titre/rate/yield metrics and products' spectrum. Alternatively, when the first stage is abiotic, the integrated two‐stage scheme foresees, in the first stage, the catalytic transformation of CO 2 into C 1 products that, in the second stage, can be metabolized by acetogens. This latter scheme leverages the metabolic flexibility of acetogens in efficient utilization of the products of CO 2 abiotic hydrogenation, namely formate and methanol, to synthesize multicarbon compounds but also to act as flexible catalysts for hydrogen storage or production.

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Section: Biological Two‐stage Processes For Products' Synthesis From ...mentioning

confidence: 99%

Leveraging substrate flexibility and product selectivity of acetogens in two‐stage systems for chemical production

Ricci

Seifert²,

Bernacchi³

et al. 2022

Microbial Biotechnology

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“…Instead, natural formatotrophs could prove to be valid platforms for developing formate-based bioproduction. The facultative chemo-litho-autotroph C. necator is an ideal candidate to explore for this scope, as it is a capable of growing on formate as the sole carbon and energy source using the CBB cycle (Brigham, 2019; Grunwald et al, 2015; Li et al, 2012; Liu et al, 2016; Panich et al, 2021; Pavan et al, 2022; Raberg et al, 2018; Sohn et al, 2021; Volodina et al, 2016). This bacterium oxidizes formate to CO 2 while reducing NAD + to NADH through a kinetically fast ( k cat of 200 s -1 ) molybdenum-dependent formate dehydrogenase (Fdh) encoded by fdsGBACD (Niks et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Engineering the biological conversion of formate into crotonate inCupriavidus necator

Collas¹,

Dronsella

Kubis³

et al. 2023

Preprint

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To advance the sustainability of the biobased economy, our society needs to develop novel bioprocesses based on truly renewable resources. The C1-molecule formate is increasingly proposed as carbon and energy source for microbial fermentations, as it can be efficiently generated electrochemically from CO2 and renewable energy. Yet, its biotechnological conversion into value-added compounds has been limited to a handful of examples. In this work, we engineered the natural formatotrophic bacterium C. necator as cell factory to enable biological conversion of formate into crotonate, a platform short-chain unsaturated carboxylic acid of biotechnological relevance. First, we developed a small-scale (150-mL working volume) cultivation setup for growing C. necator in minimal medium using formate as only carbon and energy source. By using a fed-batch strategy with automatic feeding of formic acid, we could increase final biomass concentrations 15-fold compared to batch cultivations in flasks. Then, we engineered a heterologous crotonate pathway in the bacterium via a modular approach, where each pathway section was assessed using multiple candidates. The best performing modules included a malonyl-CoA bypass for increasing the thermodynamic drive towards the intermediate acetoacetyl-CoA and subsequent conversion to crotonyl-CoA through partial reverse β-oxidation. This pathway architecture was then tested for formate-based biosynthesis in our fed-batch setup, resulting in a two-fold higher titer, three-fold higher productivity, and five-fold higher yield compared to the strain not harboring the bypass. Eventually, we reached a maximum product titer of 148.0 ± 6.8 mg/L. Altogether, this work consists in a proof-of-principle integrating bioprocess and metabolic engineering approaches for the biological upgrading of formate into a value-added platform chemical.

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“…Ralstonia eutropha (also known as Cupriavidus necator ) is a bacterium that has been cultivated to produce PHA from various waste streams (Sohn et al, 2021 ). This bacterium has been used to produce PHA from plant‐based oleaginous waste streams such as waste frying oil and oil from coffee grounds (Brigham & Riedel, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Continuous feeding strategy for polyhydroxyalkanoate production from solid waste animal fat at laboratory‐ and pilot‐scale

Gutschmann

Simões

Schiewe

et al. 2022

Microbial Biotechnology

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Bioconversion of waste animal fat (WAF) to polyhydroxyalkanoates (PHAs) is an approach to lower the production costs of these plastic alternatives. However, the solid nature of WAF requires a tailor‐made process development. In this study, a double‐jacket feeding system was built to thermally liquefy the WAF to employ a continuous feeding strategy. During laboratory‐scale cultivations with Ralstonia eutropha Re2058/pCB113, 70% more PHA (45 g PHA L −1 ) and a 75% higher space–time yield (0.63 g PHA L −1 h −1 ) were achieved compared to previously reported fermentations with solid WAF. During the development process, growth and PHA formation were monitored in real‐time by in‐line photon density wave spectroscopy. The process robustness was further evaluated during scale‐down fermentations employing an oscillating aeration, which did not alter the PHA yield although cells encountered periods of oxygen limitation. Flow cytometry with propidium iodide staining showed that more than two‐thirds of the cells were viable at the end of the cultivation and viability was even little higher in the scale‐down cultivations. Application of this feeding system at 150‐L pilot‐scale cultivation yielded in 31.5 g PHA L −1 , which is a promising result for the further scale‐up to industrial scale.

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Chemoautotroph Cupriavidus necator as a potential game-changer for global warming and plastic waste problem: A review

Cited by 65 publications

References 137 publications

Leveraging substrate flexibility and product selectivity of acetogens in two‐stage systems for chemical production

Leveraging substrate flexibility and product selectivity of acetogens in two‐stage systems for chemical production

Engineering the biological conversion of formate into crotonate inCupriavidus necator

Continuous feeding strategy for polyhydroxyalkanoate production from solid waste animal fat at laboratory‐ and pilot‐scale

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