Chemo-metric assisted UV-spectrophotometric methods for simultaneous estimation of Darunavir ethanolate and Cobicistat in binary mixture and their tablet formulation

Singh, Veena D.; Singh, Vijay Kumar

doi:10.1016/j.saa.2020.119383

Cited by 8 publications

(2 citation statements)

References 15 publications

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“…The mixture was homogenized using mortar and pestle, and prepared samples were subjected to UVspectrophotometry using the same procedure as mentioned in "analysis of tablets" for simultaneous equation and firstorder derivative methods as well as chemometric methods. 32…”

Section: Selectivitymentioning

confidence: 99%

Development and Validation of Analytical Methods for Simultaneous Estimation of Lercanidipine Hydrochloride and Enalapril Maleate

Patel

2023

Int J Life Sci Pharm Res

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The fixed dose of lercanidipine hydrochloride(LH) and enalapril maleate (EM) is used to provide more effective control of hypertension. The research work was aimed at the development and validation of simple and sensitive analytical methods for their quantification from tablets. The simultaneous equation, first-order derivative, and multivariate spectrophotometric methods were developed for the simultaneous estimation of LH and EM in tablets. The simultaneous equation method involves a determination of LH and EM at 236 nm and 207 nm, respectively. First order derivative UV spectrophotometry method involves using a zero crossing point of LH at 330 nm and EM at 219 nm. Both methods were validated as per ICH Q2 (R2) guidelines and found to be accurate and precise as they exhibited <2% relative standard deviation. The limit of detection (LOD) and limit of quantitation (LOQ) for LH were found to be 0.26 μg/ml and 0.78 μg/ml respectively whereas LOD and LOQ for EM were found to be 0.41 μg/ml and 1.24 μg/ml respectively for simultaneous equation method. The LOD and LOQ for LH were found to be 0.39 μg/ml and 1.19 μg/ml respectively whereas LOD and LOQ for EM were found to be 0.31 μg/ml and 0.97μg/ml respectively for the first-order derivative method. Three Chemometric methods namely classical least square (CLS), principal component regression (PCR), and partial least square (PLS) were studied for the simultaneous determination of LH and EM in tablets using spectrophotometry. A set of 25 standard mixtures containing both drugs were prepared in the range of 5–25 μg/ml for both drugs. The analytical figure of merit (FOM) was determined for all three chemometric methods. The LOD values for LH were found to be 0.97, 0.93, and 0.94 μg/ml and 0.90,0.88, and 0.89 μg/ml for EM using CLS, PCR, and PLS modeling techniques. The proposed methods were user-friendly, rapid, and sensitive enough for the determination of LH and EM.

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Section: Selectivitymentioning

confidence: 99%

Development and Validation of Analytical Methods for Simultaneous Estimation of Lercanidipine Hydrochloride and Enalapril Maleate

Patel

2023

Int J Life Sci Pharm Res

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“…Chemometrics techniques have a variety of uses in analytical chemistry based on spectroscopy, including UV-visible spectrophotometry [7][8][9][10][11][12][13][14][15][16][17][18][19] , fluorescence spectroscopy [20][21][22][23], NIR spectroscopy [24][25][26][27], and the FTIR spectroscopic method [28]. In addition, chromatography techniques like liquid chromatography [29][30][31] and a variety of other analytical chemistry techniques, such as flow-injection analysis, are accessible for pharmaceutical formulations [32,33].…”

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confidence: 99%

Development and validation of a new spectrophotometric method for simultaneous determination of spiramycin and metronidazole in tablet pharmaceutical dosage forms using chemometrics technique in comparison with HPLC

Almaqtari,

Al-Odaini,

Alarbagi

et al. 2023

jast

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A new, easy, fast, and cost-effective simultaneous spectrophotometric method for determining the combined concentration of spiramycin and metronidazole in tablet pharmaceutical formulations was developed and proposed. The suggested method was validated using two chemometric techniques, namely partial least square regression (PLS) and principal component regression (PCR). The significance of the proposed method is that pretreatment or separation steps are not required. Various drug concentrations and instrumental spectra of 25 mixed solutions of spiramycin and metronidazole were used for model design and validation. The UV analysis of the prepared mixtures was recorded with respect to a selected solvent blank in the wavelength range of 200–375 nm. The digitized absorbance was sampled at 0.2 nm intervals. The R2 values of 1 and 0.9998 assigned for the PLS of spiramycin and metronidazole, and those of 0.9991 and 0.9998 for the PCR of spiramycin and metronidazole, respectively, exhibited greater prediction efficiencies. The results were statistically compared to those of the HPLC reference method. The statistical comparison between the recommended (PLS and PCR) and reference HPLC method did not find any appreciable variations in accuracy or precision. The suggested approach is simple, effective, and trustworthy enough to be employed as an alternative analytical method for quality control of drug analysis in the pharmaceutical industry.

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Chemometrics on pharmaceuticals

Maggio,

Vignaduzzo,

Antonio

2024

Chemometrics

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Chemo-metric assisted UV-spectrophotometric methods for simultaneous estimation of Darunavir ethanolate and Cobicistat in binary mixture and their tablet formulation

Cited by 8 publications

References 15 publications

Development and Validation of Analytical Methods for Simultaneous Estimation of Lercanidipine Hydrochloride and Enalapril Maleate

Development and Validation of Analytical Methods for Simultaneous Estimation of Lercanidipine Hydrochloride and Enalapril Maleate

Development and validation of a new spectrophotometric method for simultaneous determination of spiramycin and metronidazole in tablet pharmaceutical dosage forms using chemometrics technique in comparison with HPLC

Chemometrics on pharmaceuticals

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