Chemo-enzymatic saccharification and bioethanol fermentation of lipid-extracted residual biomass of the microalga, Dunaliella tertiolecta

Lee, Ok Kyung; Kim, A Leum; Seong, Dong Ho; Lee, Choul‐Gyun; Jung, Yeon Tae; Lee, Jinwon; Lee, Eun Yeol

doi:10.1016/j.biortech.2013.01.007

Cited by 138 publications

(34 citation statements)

References 19 publications

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“…remaining carbohydrates. In the case of Dunaliella, the carbohydrate is mostly present as starch (Lee et al, 2013a). These carbohydrates have been successfully saccharified into glucose, and the resulting saccharification broth was used for bioethanol fermentation without any pretreatment (Lee et al, 2013a).…”

Section: Introductionmentioning

confidence: 99%

Bioethanol production from carbohydrate-enriched residual biomass obtained after lipid extraction of Chlorella sp. KR-1

Lee

2015

Bioresource Technology

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Section: Introductionmentioning

confidence: 99%

Bioethanol production from carbohydrate-enriched residual biomass obtained after lipid extraction of Chlorella sp. KR-1

Lee

2015

Bioresource Technology

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“…Kim et al ., used Chlorella vulgaris microalgae as a feedstock for bioethanol production by using S. cerevisiae yeast and arranged the pH of the yeast growth medium as 5 for both batch type fermentation and continuous fermentation with immobilized yeast cells in their study . In another study, which is performed by halophilic Dunaliella tertiolecta microalgae as a sugar source for the bioethanol production, the researchers arranged the pH of the growth medium after the enzymatic saccharification process as 6.5 for the 5‐l fermenter experiment for S. cerevisiae . The pH value that is necessary for the P. stipitis yeast is in the same range with S. cerevisiae .…”

Section: Resultsmentioning

confidence: 99%

Usage of thermophilic cyanobacterial biomass for bioethanol production

Karatay

2014

Env Prog and Sustain Energy

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Although the usage of microalgae biomass as a feedstock for bioethanol production has been extensively studied, very little attention has been given to the usage of cyanobacterial biomass for bioethanol production. In this study, a thermophilic cyanobacterial strain namely Phormidium sp. was used for producing bioethanol. Some important parameters for bioethanol production were optimized. When the initial sugar concentration was 8.69 at 40 g L 21 cyanobacteria loading, Pichia stipitis used 31.5% of the sugar and produced 1.64 g L 21 ethanol and Saccharomyces cerevisiae used 34.6% of the sugar and produced 1.97 g L 21 ethanol at the end of 72 h. The highest bioethanol concentration was obtained as 2.32 g L 21 for S. cerevisiae and 1.7 g L 21 for P. stipitis at 24 h of incubation time and substrate concentration of 40 g L 21 cyanobacterial biomass at pH 5. This study demonstrated that the biomass of a thermophilic cyanobacteria is a promising feedstock for bioethanol production.

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“…Lee et al () used Viscozyme L or amyloglucosidase (AMG 300L) to disrupt 5% (w/w DW ) residual biomass of Dunaliella tertiolecta after lipid extraction. Viscozyme L is a commercial cocktail containing carbohydrases including β‐glucanases, cellulases, hemicellulases, xylanases, and arabinases activities.…”

Section: Cell Wall‐degrading Enzymes Employed For Microalgae Cell Dismentioning

confidence: 99%

Enzymatic cell disruption of microalgae biomass in biorefinery processes

Demuez

Mahdy

Tomás‐Pejó

et al. 2015

Biotech & Bioengineering

142

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When employing biotechnological processes for the procurement of biofuels and bio-products from microalgae, one of the most critical steps affecting economy and yields is the "cell disruption" stage. Currently, enzymatic cell disruption has delivered effective and cost competitive results when compared to mechanical and chemical cell disruption methods. However, the introduction of enzymes implies additional associated cost within the overall process. In order to reduce this cost, autolysis of microalgae is proposed as alternative enzymatic cell disruption method. This review aims to provide the state of the art of enzymatic cell disruption treatments employed in biorefinery processes and highlights the use of endopeptidases. During the enzymatic processes of microalgae life cycle, some lytic enzymes involved in cell division and programmed cell death have been proven useful in performing cell lysis. In this context, the role of endopeptidases is emphasized. Mirroring these natural events, an alternative cell disruption approach is proposed and described with the potential to induce the autolysis process using intrinsic cell enzymes. Integrating induced autolysis within biofuel production processes offers a promising approach to reduce overall global costs and energetic input associated with those of current cell disruption methods. A number of options for further inquiry are also discussed.

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Chemo-enzymatic saccharification and bioethanol fermentation of lipid-extracted residual biomass of the microalga, Dunaliella tertiolecta

Cited by 138 publications

References 19 publications

Bioethanol production from carbohydrate-enriched residual biomass obtained after lipid extraction of Chlorella sp. KR-1

Bioethanol production from carbohydrate-enriched residual biomass obtained after lipid extraction of Chlorella sp. KR-1

Usage of thermophilic cyanobacterial biomass for bioethanol production

Enzymatic cell disruption of microalgae biomass in biorefinery processes

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