Chemistry, oxidative stability and bioactivity of oil extracted from Rosa rugosa (Thunb.) seeds by supercritical carbon dioxide

Grajzer, Magdalena; Wiatrak, Benita; Gębarowski, Tomasz; Matkowski, Adam; Grajeta, Halina; Rój, Edward; Kulma, Anna; Prescha, Anna

doi:10.1016/j.foodchem.2020.127649

Cited by 20 publications

(15 citation statements)

References 41 publications

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“…The gas chromatographic separation of FAMEs was performed on a SPTM-2560 fused silica capillary column (Supelco, USA, 100 m × 0.25 mm × 0.2 µm) using an Agilent GC 6890N gas chromatograph coupled with a FID detector (Agilent Technologies, Santa Clara, CA, USA). Detailed chromatographic separation conditions and the program were described previously [21].…”

Section: Fatty Acid Compositionmentioning

confidence: 99%

Characteristics and Antioxidant Potential of Cold-Pressed Oils—Possible Strategies to Improve Oil Stability

Grajzer

Szmalcel

Kuźmiński

et al. 2020

Foods

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The relations of the antiradical capacity to oxidative stability parameters and the contents of fatty acids, sterols, tocopherols, phenols, flavonoids, chlorophyll, Cu, and Fe were assessed in 33 cold-pressed seed oils: Walnut (7 brands of oils), rosehip (3), camelina (6), milk thistle (5), flax (6), and pumpkin (6). The antiradical capacity of oils depended strongly on tocopherol contents with a synergistic effect with polyphenols. The efficacy of tocopherols in cold-pressed oils was accompanied by a negative correlation of their antioxidant capacity with the peroxide value increase after 3 months of shelf life. This study also showed a positive correlation between the content of phytosterols and the antiradical capacity in the lipophilic fraction of cold-pressed oils rich in n-3 polyunsaturated fatty acids (PUFAs). Multiple regression analysis identified groups of antioxidants naturally occurring in cold-pressed oils in relation to their fatty acid composition, which added to the cold-pressed oils could provide possible strategies to improve their stability. Achieving high stability is primarily a result of high phytosterol content exceeding the molar ratio of 1:100 for total phytosterols to α-linolenic acid. However, the molar ratios of tocopherols to linoleic acid below 1:2000 and polyphenols to linoleic acid below 1:3000 does not prevent oxidation in oils with the predominance of linoleic acid.

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Section: Fatty Acid Compositionmentioning

confidence: 99%

Characteristics and Antioxidant Potential of Cold-Pressed Oils—Possible Strategies to Improve Oil Stability

Grajzer

Szmalcel

Kuźmiński

et al. 2020

Foods

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“…NHDF cell line was maintained in RPMI-1640 culture medium with supplemented material including 10% FBS, 1% mixture of antibiotic/antimycotic, 0.01 M of HEPES, 0.02 M of L-glutamine, and 0.001 M of sodium pyruvate. Finally, it was incubated in an incubator (at 37°C and a humidified atmosphere with 5% CO 2 ) [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

In Vitro and In Vivo Wound Healing Activity of Astragalus floccosus Boiss. (Fabaceae)

Akbari

Azadbakht

Bagheri

et al. 2022

Advances in Pharmacological and Pharmaceutical Sciences

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Estrogens are a group of sex hormones which have receptors on the skin and lead to increased cells and wound healing. Normally isoflavonoids are present in Astragalus floccosus Boiss. (Leguminosae). Therefore, the present study was conducted to evaluate the presence of isoflavonoids in A. floccosus’ rich fraction of flavonoid and evaluate its wound healing effect accordingly. Flavonoids were evaluated by LCMS. Scratch was conducted and the medium culture was treated with the Astragalus’ rich fraction of flavonoid (RFF) and was compared with nontreated culture during 48 hours. In addition, in vivo full-thickness wound healing evaluation was performed on rats. The rats were put into four groups and treated on a daily basis for 21 days with a cream containing 1.5% of the RFF (group 1), silver sulfadiazine (group 2), and Vaseline (group 3) separately. The nontreated group (group 4) was created for a better comparison. During the examination, wound size was evaluated and histopathological examination was performed. Herbal analysis detected 11 flavonoids, including 2 isoflavonoids, Calycosin-7-O-beta-D-glucoside and Formononetin, in the RFF. In vitro scratch wound healing showed significant improvement with RFF treatment in comparison to nontreated medium. Furthermore, in vitro drug release of Astragalus ointment showed a stationary line during 24 h and 0.14 mg/ml of flavonoid penetrated the skin. In vivo wound size evaluation showed significant improvement in the group treated with the RFF in comparison to other groups. Histopathological results indicated that congestion, edema, inflammation, necrosis, and angiogenesis decreased during the examination and fibroblast proliferation fibrosis epithelization was increased especially in the RFF group in comparison to the silver sulfadiazine and free groups. In conclusion, A. floccosus showed that wound healing activity in both in vitro and in vivo analyses can be attributed to the presence of isoflavonoids with estrogen-like activity in this plant.

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“…NHDF cell line was maintained in RPMI-1640 culture medium with supplemented material including 10% FBS, 1% mixture of antibiotic/antimycotic, 0.01 M of HEPES, 0.02 M of L-glutamine, and 0.001 M sodium pyruvate. Subsequently, it was incubated in an incubator (37°C, humidified atmosphere with 5% CO2) [ 25 ].…”

Section: Methodsmentioning

confidence: 99%

In Silico, In Vitro, and In Vivo Wound Healing Activity of Astragalus microcephalus Willd.

Akbari

Azadbakht

Bagheri

et al. 2022

Advances in Pharmacological and Pharmaceutical Sciences

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Background and Objectives. A wound is one of the high-prevalence disorders that affect people’s lives physically, mentally, and financially. This study examined the Astragalus microcephalus Willd. wound healing process in in vivo and in vitro conditions by focusing on the phytoestrogen activity of this extract. Methods. The methanolic root extract was prepared by maceration, and flavonoids were evaluated by LC/MS. In silico examination was performed based on the LC/MS results, and the binding affinity of these compounds to estrogen receptors (ERs) α and β was evaluated. Wound healing evaluation in both in vitro (NHDF cell line, by 500 μg/ml concentration of the extract, 24 h) and in vivo (Wistar rat, topical daily treated with 1.5% of the extract ointment, 21 days) conditions in comparison to control groups was conducted. Rats’ control groups included silver sulfadiazine, Vaseline, and the nontreated groups. Results. Eleven flavonoids were detected using LC/MS. The in silico study showed that formononetin, kaempferol-based structures, quercetin-3-O-neohesperidoside, and calycosin-7-O-beta-D-glucoside had a high affinity (<−6.3) to ERs α and β. Wound closing measurement showed significant improvement in the group treated with the extract in both in vitro and in vivo assays compared to the control groups. Histopathological results confirmed these findings; inflammation factors decreased, and fibroblast proliferation, fibrosis, and epithelization increased, especially in the extract group. Conclusion. This study shows that Astragalus microcephalus has wound healing activity in vitro and in vivo with low toxicity due to the presence of flavonoids, especially isoflavonoids, which show a high affinity to bind to ERs α and β in the skin tissue.

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Chemistry, oxidative stability and bioactivity of oil extracted from Rosa rugosa (Thunb.) seeds by supercritical carbon dioxide

Cited by 20 publications

References 41 publications

Characteristics and Antioxidant Potential of Cold-Pressed Oils—Possible Strategies to Improve Oil Stability

Characteristics and Antioxidant Potential of Cold-Pressed Oils—Possible Strategies to Improve Oil Stability

In Vitro and In Vivo Wound Healing Activity of Astragalus floccosus Boiss. (Fabaceae)

In Silico, In Vitro, and In Vivo Wound Healing Activity of Astragalus microcephalus Willd.

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