Chemistry of Viruses

Knight, C.A.

doi:10.1007/978-3-7091-3328-6

Cited by 24 publications

(1 citation statement)

References 491 publications

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“…The viral nucleic acid, DNA and RNA are sensitive to extreme pH. Nucleic acid structure alters at pH below three or above 10 [ 3 ]. The other factor affecting nucleic acid stability is the nuclease enzymes- deoxyribonuclease and ribonuclease.…”

Section: Introductionmentioning

confidence: 99%

From cold chain to ambient temperature: transport of viral specimens- a review

Dsa,

Kadni,

2023

Annals of Medicine

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The diagnosis of an aetiology is dependent on the collection, transport, and storage of the infectious sample. The transport of the sample plays a crucial role in the chain of diagnosis. It is important to maintain the biological integrity of the pathogen during the transport of the sample to achieve an accurate diagnosis. This is important, particularly for labile organisms like viruses that are inactivated easily compared to other microorganisms. Many transport media have been utilised to ensure the integrity of the virus during transport. While most of the transport media are focused on preserving the infectious properties of the virus, progress has been made to develop virus transport media to inactivate the virus and obtain the stability of the viral nucleic acid, enabling better molecular diagnosis of the virus aetiologies. This review summarises the various media used for the transport of virus samples and focuses on the need to develop virus transport media that inactivates the virus and preserves the viral nucleic acid.

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Section: Introductionmentioning

confidence: 99%

From cold chain to ambient temperature: transport of viral specimens- a review

Dsa,

Kadni,

2023

Annals of Medicine

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Phytopathogene Viren

Wittmann¹,

Sänger²

1965

Progress in Botany

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Purification of pea enation mosaic virus and the infectivity of its components

Mahmood¹,

Peters²

1973

Netherlands Journal of Plant Pathology

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A slightly modified procedure for purifying pea enation mosaic virus (PEMV) is described. Essential features are the adding of Mg + + ions to the extraction buffer and the use of polyethylene glycol for concentrating the virus; Mg ++ ions seem to protect virus infectivity. Both components of PEMV were found to be infectious. The infectivity of each component in its band on a sucrose gradient was directly proportional to its optical density. The infectivity was not enhanced when both components were simultaneously inoculated onto Chenopodiurn album. It is concluded that each component carries a complete genome of the virus.

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Chemistry of Viruses

Cited by 24 publications

References 491 publications

From cold chain to ambient temperature: transport of viral specimens- a review

From cold chain to ambient temperature: transport of viral specimens- a review

Phytopathogene Viren

Purification of pea enation mosaic virus and the infectivity of its components

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